Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"droplet sorting"”.
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Ultrahigh-Throughput Microfluidic Droplet Screening of Metagenomic Libraries for Esterases and Kemp Eliminases
… rare (1 in 105 on average). Ultrahigh-throughput droplet microfluidics has emerged as an effective technology to overcome this limitation, with the ability to screen 107 reactions per day. However, fewer than ten enzyme assays have been implemented in droplets to date and only once has droplet …
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Strategies to Roam Enzymatic Fitness Landscapes in Ultrahigh-Throughput
… by the development of (water-in-oil emulsion) droplet technology, miniaturizing the reaction vessel to a single droplet of ~pL volume, thus enabling 1 billion parallel reaction vessels per mL in which unique enzymes are assessed for product formation. Droplet technology has been limited by its …
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Screening of algal cells using microdroplet technology
… study of algal cells at the single cell level. Droplet microfluidics has been established as a multifunctional platform, with many advantages for the study of biological systems in the microscale. In this thesis, droplet microfluidic technology was used to optimise and expand the application of …
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Droplet levitation and underwater plastron restoration using aerophilic surface textures
… for reducing the contact line pinning of water droplets on the surface and lowering the skin friction drag experienced by the surface underwater. However, these aerophilic textures are prone to impregnation by water due to turbulent pressure fluctuations from external flows and dissolution of …
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Characterising fitness landscapes of protein evolution by next-generation sequencing
… so I optimised the conditions for better sorting resolution using the mKate2 fluorescent protein as an expression reporter. In the second iteration of the activity sorting I additionally included UMIs in the plasmid design to improve the utilisation of NGS capacity. In the case of PTE, I …
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Exploring protein fitness landscapes with new high-throughput technologies
… of enzyme function, such as absorbance-activated droplet sorting, currently lack the sensitivity of more specific fluorescence-based or low-throughput counterparts. A universal approach to increase detectability in single cell-lysate microfluidic enzyme assays is established by amplifying the …
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Microfluidic Engineering for Ultrasensitive Molecular Analysis of cells
… examine the temporal dynamics in the process. Droplets with encapsulated beads and cells have been increasingly used for studying molecular and cellular biology. However, a mixed population of droplets with an uneven number or type of encapsulated particles is resulted and used for screening. …
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Functional metagenomic discovery and characterisation of CAZymes by microfluidic methods
… format, i.e. employing small water-in-oil droplets as reaction vessels that can be screened in ultrahigh throughput, has already allowed many standard lab procedures to be sped up, including functional screenings. Nevertheless, microfluidic techniques for functional metagenomic enzyme …
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High-throughput protein engineering tools for efficient exploration and quantitative mapping of protein fitness landscapes
… with Unbiased Microfluidic Emulsion Sorting and Sequencing), which enables quantitative enzymatic activity measurements in vitro at ultra-high throughput. By replacing single cells with monodisperse hydrogel beads displaying individual enzyme variants at precisely controlled …