Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 93 for “"double-stranded RNA"”.
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Double-stranded RNA uptake in Caenorhabditis elegans
dsRNA uptake from the environment is a common phenomenon in many invertebrates and can be harnessed for acquiring pest control. In the nematode Caenorhabditis elegans, a transmembrane protein named systemic RNAi deficient-2 (sid-2) is required for dsRNA uptake from the environment through the …
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The Mechanism of Double-Stranded RNA Response in Neurospora
In eukaryotic cells, recognition of double-stranded RNA (dsRNA) by the enzyme Dicer initiates the RNA interference (RNAi) pathway, resulting in post-transcriptional gene silencing. Argonaute proteins play a critical role in this conserved pathway, which is present in protists, fungi, plants and …
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Determining the mechanism of double-stranded RNA-induced cell death in ovarian cancer.
… tumors pathogen-derived ligands, such as dsRNA, can activate pattern recognition receptors (PRRs) that are capable of inducing apoptosis. In this dissertation we have found that in ovarian cancer cell lines (DOV-13, SKOV-3, CAOV-3, and OVCAR-3), dsRNA treatment alters cell survival. When …
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Reading Between the Strands: Deciphering Double Stranded RNA-Based Immune Responses in Mosquito-Virus Interactions
… pathogen-associated molecular patterns, with double-stranded RNA (dsRNA), a replication intermediate of many viruses, serving as a key immunostimulatory signal. While RNA interference (RNAi) remains the most well-characterized antiviral pathway triggered by dsRNA, accumulating evidence …
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RNA EDITING INDEPENDENT FUNCTIONS OF ADENOSINE DEAMINASES ACTING ON DOUBLE-STRANDED RNA IN HEPATOCELLULAR CARCINOMA
ADENOSINE DEAMINASES ACTING ON DOUBLE-STRANDED RNA (DSRNA) (ADARS) IS A FAMILY OF ENZYMES CATALYSING ADENOSINE TO INOSINE CONVERSION (A-TO-I EDITING). IF HAPPENED IN CODING REGIONS, A-TO-I EDITING CAN CAUSE PROTEIN RECODING. RECODING EDITING HAS BEEN REPORTED TO CONTRIBUTE TO HEPATOCELLULAR …
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IDENTIFICATION OF FACTORS INVOLVED IN DOUBLE STRANDED RNA-INDUCED CELL DEATH THROUGH GENOME-WIDE CRISPR SCREENS
Double-stranded RNAs (dsRNAs) are potent immunostimulatory nucleic acids of viral origin but also physiologically produced by mammalian cells. Increased levels of dsRNAs, due to viral infection or failure of endogenous repression, trigger an immune response that leads to cytokines production or …
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Reprogramming the expression of the double-stranded RNA mitovirus OnuMV1c from the mitochondria to the cytoplasm in the fungal pathogen Ophiostoma novo-ulmi
… is a mitochondrial virus and has a 3.1 kb single-stranded positive RNA genome that encodes an RNA-dependent RNA polymerase (RdRp) involved in its replication as a double-stranded RNA molecule. It exists in O. novo-ulmi mitochondria in both its single-stranded and double-stranded forms. Our …
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Development and characterization of an inducible RNAi system in Toxoplasma gondii
… mammalian or avian cell. The ability of double stranded RNA to downregulate gene expression has been demonstrated in T. gondii. We have constructed a tetracycline based inducible RNAi vector which can be used for regulating gene expression in T. gondii. Upon the addition of …
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Regulation of escherichia coli ribonuclease III by the bacteriophage T7 protein kinase
Ribonuclease III (RNase III) is a double-stranded-RNA-specific nuclease of Escherichia coli. RNase III functions as a homodimer. The 25.6 kDa subunit contains two domains, a 148 residue catalytic domain and a 78 residue double-stranded-RNA-binding domain (dsRBD). RNase III is involved in the …
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Investigation of the roX RNAs and the RNA Helicase MLE in Dosage Compensation in Drosophila melanogaster
… and is composed of the two noncoding roX (RNA on the X) RNAs and at least five proteins, including the RNA helicase Maleless (MLE). MLE contains two highly conserved double stranded RNA binding domains (DRBDs) at the N terminus. We investigated the roles of the roX RNAs and MLE helicase …
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Regulatory Mechanism of the RNAi Pathway
RNA interference (RNAi) is post-transcriptional gene silencing initiated by Dicer, a RNase III that processes double-stranded RNA (dsRNA) precursors into small interfering RNA (siRNA). In Drosophila, Dicer2 and R2D2 coordinately recruit duplex siRNA to the effector RNA-induced silencing complex …
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Strategic modification of oligonucleotides with carboxylic acids containing small molecules
Small interfering RNA (siRNA) are double-stranded RNA molecules used to manipulate gene regulation, by silencing gene expression. SiRNA molecules have been integrated into the therapeutic field, with several FDA-approved medications targeting liver-specific diseases. While effective, these …
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Functional genomic studies of regeneration in the planarian Schmidtea mediterranea
… for each gene. Functional analysis using double-stranded RNA-mediated RNA interference (RNAi) was implemented to determine effects on regeneration. The hypothesis was that effectors of regeneration are only conserved in animals capable of somatic tissue regeneration as adults. No genes …
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RNAi-related genes in Toxoplasma gondii.
RNA interference (RNAi) is an evolutionarily conserved mechanism through which double-stranded RNA (dsRNA) triggers degradation of homologous transcripts. Argonaute proteins and small interfering RNAs (siRNAs) are the known signature components of the RNAi effector complex, named RNA-induced …
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Characterization of Attachment and Receptor Isolation of Infectious Pancreatic Necrosis Virus to Chse-214 Cells
… Necrosis Virus (IPNV) is a bisegmented, double stranded RNA virus with a protein capsid and no envelope and is classified in the group, Birnaviridae. Although much is known about viral genes and proteins, the initial stages of attachment to susceptible cells are not known and are the …
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Techniques of RNA Isolation and Characterization in Infectious Pancreatic Necrosis Virus-Infected Chinook Salmon Embryo Cells
… for fish hatcheries. This prototype naked, double-stranded RNA virus of the Birnaviradae family was studied to test new techniques for isolation and characterization of its ambiguous early post-infection viral RNA products. RNA was extracted by either the classical phenol method or a new kit …
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Mobile RNA reveal differential requirements for gene silencing in C. elegans at single-cell resolution
Delivery of double-stranded RNA (dsRNA) into animals can silence genes of matching sequence in diverse cell types through mechanisms that have been collectively called RNA interference. In the worm C. elegans, such organism-wide silencing relies on the transport of dsRNA to most cells and requires …
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Translational regulation by short RNAs in mammalian cells
… are transcribed in the nucleus into messenger RNA, spliced, and exported to the cytoplasm, where they are translated into proteins. It was therefore a profound surprise when RNA itself, in the form of [approx.]21nt short RNAs, was discovered to have major generalized roles in the regulation of …
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Branching into RNAi: Synthesis, Characterization and Biology of Branch and Hyperbranch siRNAs
… in cancer research in the advent of the first RNA interference (RNAi) application in humans. RNAi solicits the use of a double-stranded RNA substrate, aptly named short-interfering RNA (siRNA), which binds to and triggers the degradation of a targeted complementary mRNA strand within the …
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