Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 180 for “"double-strand breaks"”.
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Repair of DNA Double-Strand Breaks in Chromosomes: Impact of Telomerase
No abstract prepared.
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ATM-Dependent ERK Signaling in Response to DNA Double Strand Breaks
… events. To generate radio-mimetic DNA double-strand breaks (DSBs) without and minimizing the effects on extra-nuclear radiation targets, human (p53+) glioma and carcinoma cells containing bromodeoxyuridine (BrdU)- substituted DNA were treated with Hoechst 33258 followed by long …
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The Role of E2F1 In The Response to Dna Double Strand Breaks
… also relocalizes and forms foci at sites of DNA double-strand breaks but the function of E2F1 at sites of damage is still unknown. Here I reveal that E2F1 deficiency leads to increased spontaneous DNA break and impaired recovery following exposure to ionizing radiation. In response to DNA …
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Phopsphorylation and Ubiquitin Modification At Dna Damage Sites In Response to Double-Strand Breaks
… signaling at the DNA damage sites.</p> <p>DNA double-strand breaks triggers a phosphorylation-mediated signaling at the damage sites leading to histone ubiquitination in Lys63-linked manner that recruits BRCA1-A complex to the damage sites. The A complex is comprised of BRCA1, Rap80, NBA1, BRE, …
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Quantitative measurement and modeling of the DNA damage signaling network : DNA double-strand breaks
DNA double-strand breaks (DSB) are one of the major mediators of chemotherapy-induced cytotoxicity in tumors. Cells that experience DNA damage can initiate a DNA damage-mediated cell-cycle arrest, attempt to repair the damage and, if successful, resume the cell-cycle (arrest/repair/resume). Cells …
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A novel DNA damage quantification platform enables high throughput screening for genes that impact DNA double strand breaks
DNA is the blueprint of life, and the high fidelity transmission of genetic information from parent cells to progeny is essential for an organism's viability. However, our genomes are constantly being damaged by reactive molecules generated from cellular metabolic processes or introduced from the …
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Proteomic Identification of Histone Post-Translational Modifications Induced By Dna Double-Strand Breaks and Novel Proteins Involved In The Dna Damage Response
<p>Inaccurate repair of DNA double-strand breaks (DSBs) can lead to DNA mutation and chromosome rearrangements, causing human diseases such as cancer. Although we know the basic mechanisms of DSB repair, the added complexities in the chromatin context are unclear. This is partially due to the lack …
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A novel high-throughput in-cell Western assay for the quantitative measurement of signaling dynamics in DNA damage signaling networks : cell decision processes in response to DNA double strand breaks
Following exposure to DNA damage, cells initiate a stress response involving multiple protein kinase signaling cascades. The DNA damage response results in one of several possible cell-fate decisions, or cellular responses: induction of cell-cycle arrest, initiation of DNA repair, activation of …
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Rate zonal density gradient ultracentrifugation analysis of repair of radiation damage to the folded chromosome of Escherichia coli
… measurement of the formation and repair of DNA double-strand breaks at biologically-significant doses of ionizing radiation. In vitro UV-irradiation of nucleoids resulted in an increase in the observed rate of sedimentation due to the formation of an unknown photo-product. In contrast, …
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Mechanism of Permissive Cleavage Activity of TevCas12a
… to create a dual endonuclease that made precise double-strand breaks leaving non-compatible ends for exogenous DNA insertion and specific deletions. TevCas12a was expected to make two double-strand breaks, one by I-TevI and the other from Cas12a. However, when tested in vitro, TevCas12a cut the …
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Investigating the regulation of DNA non-homologous end-joining through Ku70/80 interacting factors
DNA double-strand breaks are the most deleterious type of DNA damage that cells experience, which makes the study of double-strand break repair extremely important. Unrepaired or aberrantly repaired DNA can result in changes to core genes with critical function and thus lead to multiple diseases. …
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Synthetic Lethality of Cdk Inhibition and Doxorubicin In Triple-Negative Breast Cancer Requires P53 Inactivation
… on homologous recombination for repair of DNA double strand breaks. Notably, combination treatment increased DNA double strand breaks, while simultaneously reducing recruitment of homologous recombination proteins. Examination of isogenic immortalized human mammary epithelial cells and isogenic …
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PIKK-dependent activation of the DNA damage response by long wavelength ultraviolet radiation
… to investigate UVA and its ability to generate double strand breaks, and how the DDR is activated as a result. Cells exposed to 100 KJ m-2 UVA displayed significant amounts of DSBs, which were largely repaired within 1 hour of irradiation. γH2AX was used as a biomarker of DNA damage, and …
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Genetic and Cytological Analysis of the Spindle Assembly Checkpoint Component BUB-3 in DNA Damage Response
… is consistent with a role in dealing with DNA double-strand breaks and not with base damage. bub-3 and san-1 mutants did not exhibit a mutator phenotype, which suggests that these genes are not required to preserve genome stability in the absence of exogenous DNA damaging agents. Double mutant …
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The in Vivo Roles of the RdgB Enzyme in Escherichia Coli and the Detection of Rare Non-Canonical DNA Precursors
… The rdgB mutant of E. coli suffers DNA double-strand breaks and is a synthetic lethal mutation in combination with either the recA or recBC mutations, indicating a dependence on recombinational DNA repair. This dissertation describes the consequences of dITP incorporation into DNA in …
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How to Mend a Broken Mitochondrial Genome: Mitochondrial Recombination And Its Applications
… disease. Yet, pathways that repair animal mtDNA double-strand breaks remain uncharacterised. Double-strand breaks can be accurately repaired by homologous recombination but spontaneous mtDNA recombination is only detected rarely in animals. A system to induce and select for mtDNA recombination …
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A STUDY OF THE INTERPLAY BETWEEN SACCHAROMYCES CEREVISIAE TRANSCRIPTION AND DNA REPAIR.
… encompasses single base modifications, single strand damages and even the most toxic double strand breaks. Transcription-coupled DNA repair is involved in taking care of the single strand damages that can be induced by various chemicals including 4-nitroquinoline 1- oxide (4NQO) which forms …
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Discovery of novel systems for genome editing
… primarily induces gene knockouts by introducing double-strand breaks and relying on the cell’s error-prone repair mechanisms. Without additional elements, Cas9 lacks the capability for precise sequence insertions or specific nucleotide editing. This limitation restricts its applications to gene …
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