Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 201 for “"dominant negative"”.
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Dominant negative mutants of the human estrogen receptor
… ER mutant, V364E, is also found to be a strong dominant negative inhibitor of wild type ER transcriptional activity although, alone, it exhibits transcriptional superactivity at high levels of estradiol ($\rm10\sp{-8}\ M\ E\sb2).$ We next demonstrate that co-treatment with IBMX/CT (agents which …
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Mechanistic analysis of estrogen receptor action using dominant negative mutants
We have generated and characterized dominant negative mutants of human estrogen receptor, which are mutant forms of ER that are capable of suppressing the activity of wild-type receptor when they are co-expressed in the same cells. These mutants can be divided into two different categories: (1) The …
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Gain-of-function and dominant-negative effects of distinct p53 mutations in lung tumours
… is to characterise the gain-of-function and dominant-negative effects of p53 mutations in lung tumours and to identify ways of therapeutically targeting these p53 mutant tumours based on dependencies and susceptibilities that our analysis uncovers. To characterise the gain-of-function and …
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The splice variants of TREK-1 act as dominant-negative suppressors of human uterine relaxation: Implications for preterm labor
… individual splice variant was determined to be dominant-negative suppressors of TREK-1. The cellular distribution of TREK-1 was altered by each splice variant, which caused reduced membrane TREK-1 expression. Electrophysiological experiments confirmed the direct interaction between TREK-1 and …
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Clinical and pathological characterization of a novel transgenic animal model of diabetes mellitus expressing a dominant negative glucose-dependent insulinotropic polypeptide receptor (GIPR dn)
… animal model of diabetes mellitus expressing a dominant negative glucose-dependent insulinotropic polypeptide receptor (GIPR dn) Gastrointestinal hormones like glucose-dependent insulinotropic polypeptide have recently been shown to be involved in the pathogenesis of diabetes mellitus in humans …
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Tumor Necrosis Factor Dependent Mechanisms and Neuroprotective Strategies in Models of Parkinson's Disease
… tumor necrosis factor in vivo with a recombinant dominant-negative tumor necrosis factor inhibitor reduced 6-hydroxydopamine-induced nigral degeneration by 50% and attenuated amphetamine-induced rotational behavior, indicative of striatal dopamine preservation in a rodent model of Parkinson's …
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Role of I Kappa B Kinase Alpha and I Kappa B Kinase Beta in the Development and Function of B and T Lymphocytes
… of the immune system, transgenic mice expressing dominant negative forms of IKKα and IKKβ specifically in their B cells or T cells were generated. Phenotypic analysis of transgenic mice expressing dominant negative IKKβ in the B cells revealed that the proliferation of B cells from these mutant …
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The receptor tyrosine phosphatase Ptp69D and the receptor tyrosine kinase Pvr in Drosophila nervous system development
… in the developing visual system. Using a dominant-negative form of Ptp69D, I have identified several genes with which Ptp69D genetically interacts in photoreceptor axon targeting. Removing a single dose of the cytoplasmic tyrosine kinases Src64 or Abl or the repulsive axon guidance …
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Functional Analysis of the miR156 Regulatory Network in Arabidopsis Siliques
… mutants share certain similarities with SPL2 dominant-negative mutants and miR156 overexpression plants, including defective floral organ growth and partially sterile phenotypes. Furthermore, the transcript levels of AS2 were decreased in both miR156 overexpression plants and SPL2 …
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Functional Analysis of AtVAMP714 gene in Arabidopsis
… transcript levels were significantly reduced. Dominant negative transgenics of AtVAMP714 were also created as a method of knocking out the function of the protein and F2 generation plants were analysed for developmental defects. Consistent with the above results the dominant negative …
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Probing the organisation of the TatC component in the Tat system of <i>Escherichia coli</i>
… state of TatC, reasoning that the isolation of dominant negative TatC variants that inactivate the Tat system in the presence of a functional copy of wild type TatC would provide strong evidence TatC is an obligate oligomer. Single dominant negative TatC substitutions were isolated that were …
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Analysis of Hepatocyte Secretion Pathways: A Case Study on Hepatic Apolipoproteins, Serum Albumin, and Hepatitis C Virus
… imaging experiments. I begin by describing a dominant negative (DN) Rab GTPase screen that I performed to identify Rab proteins involved in ApoE, ApoB100 or albumin secretion. The small Rab GTPases control individual steps of vesicular transport. I analyzed how expression of individual …
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Characterization of Innate Immune Mechanisms Against RVFV and a Potential Target for Antivirals
… in human dendritic cells (DC) and to develop a dominant-negative method to attenuate RVFV NSs protein as a treatment for RVFV. Human DC displayed low permissiveness to RVFV but initiated high levels of pro-inflammatory mediator secretion in response to the virus. DCs infected with rMP12-C13type …
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PATHOGENIC MECHANISMS OF TRUNCATED TITIN IN DILATED CARDIOMYOPATHY
… evidence supporting either haploinsufficient or dominant negative mechanisms. To examine how this hypocontractility arises, we analyzed a set of 184 explanted posttransplant DCM hearts, 22 of which bore heterozygous titin truncations. To determine if truncated titin was present in these hearts, …
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The role of p27 phosphorylation in mediating atRA sensitivity of ovarian carcinoma cells
… that cannot be phosphorylated on S10 induces a dominant negative effect on the endogenous p27 activity. This dominant negative effect reverses the atRA effect on p27 binding to CDKs, on inhibition of CDK activity, on the expression of S phase genes and ultimately on the inhibition of growth of …
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A Role for RAS in V-CRK Transformation
… the characteristic p70 and p130. Expression of dominant negative Ras in the v-Crk NIH-3T3 caused morphological reversion of these cells, as well as an inhibition of colony formation in soft agar, indicating a requirement for Ras function in v-Crk transformation. However, no decrease in tyrosine …
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Lysine acetyltransferase Gcn5-B regulates the expression of crucial genes in Toxoplasma and its function is regulated through lysine acetylation
… activity (E703G). Stabilization of this dominant-negative form of GCN5b was mediated through ligand-binding to a destabilization domain (dd) fused to the protein. Induced accumulation of the ddHAGCN5b(E703G) protein led to a rapid arrest in parasite replication. Growth arrest was …
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Délétion endothéliale de SHP-1 prévient la sénescence et rétablit l’angiogenèse des cellules endothéliales vasculaires en condition de diabète
… (10 ng/mL) with or without overexpression of dominant negative of SHP-1. Four weeks after surgery, blood reperfusion, limb function (distance traveled in a running wheel) and formation of new collateral vessels in ischemic muscle were significantly reduced in DM mice compared to NDM mice. …
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Investigating NLR-mediated immunity and Pseudomonas syringae effector interactions with two MACPF domain proteins in Arabidopsis thaliana.
… was to utilize a recently published library of dominant negative NLR mutants to identify the receptor(s) involved in cad1 autoimmunity. Through screening 67 unique dominant negative transgenic lines for the suppression of cad1-5 rosette stunting and lesion formation phenotypes I did not identify …
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