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Showing 1 to 14 of 14 for “"domain swapping"”.
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Domain swapping as a molecular mechanism in amyloidosis
… have been shown to form dimers by exchange of subdomains of the monomeric proteins. This process, called ?domain swapping?, has also been suggested to play a part in the generation of amyloid fibrils. In the present work the role of this mechanism is investigated. Two variants of wild type and …
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Structural Analysis of Domain Swapping in the Protein Kinase: Crystal Structure of Human Ste20 OSR1 Kinase Domain
… by the presence of a conserved kinase domain and a noncatalytic region of great diversity at various locations. Despite recent structural studies of Ste20p kinases such as PAK1 or TAO2, the mechanism of kinase regualtion in this family still remains to be investigated. Thus, in order to …
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Regulation of Wnt signalling pathways by the Dishevelled DEP domain
… dynamic head-to-tail polymerization of its DIX domain, which are cross-linked by its DEP domain through a conformational switch from monomer to domain-swapped dimer. The domain-swapped conformation of DEP masks the site through which Dishevelled binds to Frizzled, implying that DEP domain …
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Biofilm Formation by Moraxella Catarrhalis
… analysis, site-directed mutagenesis, and domain swapping experiments indicated that the N-terminal region of UspA1 or UspA2H is likely involved in biofilm formation by M. catarrhalis.
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Computational discovery and modelling of tandem domain repeats in proteins
Domains are functional and evolutionary units of proteins that typically fold into stable globular structures. A small subset of natural multidomain proteins contain large arrays of nearly identical domains repeated in tandem, challenging some of our assumptions about protein folding and evolution. …
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Covalent modification and intrinsic disorder in the stability of the proneural protein Neurogenin 2
… factor which is stable. Here I describe domain swapping experiments between these two proteins highlighting regions conferring instability on the chimeric protein. Finally I will provide nuclear magnetic resonance (NMR) data looking at the effect of phosphorylation on protein structure in …
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Engineering a cyanobacteriochrome two-component system into a synthetic, light-controlled gene expression system for plants
… light. I demonstrate by targeted mutagenesis, domain deletions and domain swapping, that the blue-light response is due to a not previously characterised LOV-like domain. Next, I applied this insight to engineering new versions of the protein with improved light response kinetics. Further, I …
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Molecular Mechanisms in Amyloid Disorders. Novel Treatment Options in Hereditary Cystatin C Amyloid Angiopathy.
… showed the oligomers to be highly ordered, domain-swapped assemblies of cystatin C and that the oligomers could not build larger oligomers, or fibrils, without domain swapping. The stabilised oligomers were used to induce antibody formation in rabbits. Oligomer-specific antibodies were …
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The crystal structure of an aliphatic amidase from Geobacillus pallidus RAPc8
… interacting dimer interfaces by participating in domain swapping. The active site pocket has Glu, Lys, Cys catalytic triad that is conserved in the nitrilase superfamily. The substrate binding pocket is small in size, explaining the specificity of this enzyme for short aliphatic amides. These …
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The biochemistry and phylogeny of CysE: An antimicrobial target
… SATase and C-terminal left-handed β-helix domain. Additionally, we see extensive density for the C-terminal α-helical tails because of domain swapping, resulting in one of the most complete CysE crystal structures to date. The high resolution NgCysE structure was used for structure-based …
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Structure and Organization of Nuclear Pore Proteins
… Factors which affect the ordering of the FG domains were investigated. Domain swapping experiments showed that relocating the FG domains of the central nups to the cytoplasmic side caused them to become disordered, suggesting a role for local interactions in their organization. Removal of the …
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MOLECULAR CHARACTERIZATION AND EXPRESSION OF GENTISATE 1,2-DIOXYGENASE FROM PSEUDOMONAS ALCALIGENES NCIB 9867
… the two donor templates, both gene shuffling and domain swapping did not yield enzymes with increased catalytic properties. A mutant enzyme with increased catalytic activities however, was generated by random mutagenesis. The mutant enzyme had an apparent Kcat/Km ratio of 83.20 x 10-2 min-1 μM-1, …
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Regulation and Dysregulation via Docking Interactions in WNK and ERK1/2 MAPK Signaling
… the role of SPAK and OSR1 activation loop domain-swapping. I solved the structure of the inactive SPAK kinase domain, and utilized a previously identified dimerization blocking mutation to probe the role of SPAK dimerization on activation and activity. I determined that SPAK has multiple …
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Strukturbestimmung von Birkenpollenallergenen und birkenpollenassoziierten Nahrungsmittelallergenen mit NMR-Spektroskopie
… Wiesenlischgras, für das vor kurzem eine durch Domain Swapping entstandene dimere Struktur nachgewiesen wurde, zeigen die hydrodynamischen Parameter aus NMR-Relaxation, NMR-Translationsdiffusion und analytischer Ultrazentrifugation, dass sowohl apo als auch holo Bet v 4 vorwiegend monomer sind. …