Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"domain swap"”.
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Functional and structural characterisation of Mycobacterial Chaperonins
… assays, site directed mutagenesis, and domain swap experiments; I have been able to demonstrate that both chaperonins have evolved to fulfil alternate functions within the cell. I have shown that while Cpn60.2 is fully functional in E. coli, Cpn60.1 is not. However, M. tuberculosis …
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Characterization of a Putative Activation Domain in the Hulk Gene Family
… proteins encoded by these genes share conserved domain structures including a proline-rich region (PRR) in the carboxyl-terminus. Based on sequence analysis and the presence of a proline-rich domain, it has been suggested that the HULKs are putative transcription factors in which HUA2 is known to …
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Toward the understanding of TIR-NB-LRR–mediated immunity; study of the AvrRps4 recognition model in Arabidopsis
… recognizes AvrRps4 but not PopP2. Using domain swap experiments, I demonstrated that RRS1 exons 5, 6 and 7 specify PopP2 recognition. My data suggest that AvrRps4 and PopP2 interact directly with RRS1 and RRS1B. However, the exact mechanism by which these effectors activate TIR-NB-LRR R …
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Structural and functional effects of histone variant, H2A.Bbd, on the nucleosome core particle
… Major sequence differences are in the docking domain that tethers the (H2A-H2B) dimer to the (H3-H4)2 tetramer, and in the missing C-terminal region in H2A.Bbd. In this study, several biochemical and biophysical methods were used to investigate the effect of H2A.Bbd incorporation on the …
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The Specific Role of the MLL CXXC Domain in MLL Fusion Protein Function
… fusion proteins that cause leukemia. The CXXC domain of MLL is a cysteine rich DNA binding domain with specificity for binding unmethylated CpG-containing DNA. The CXXC domain is retained in oncogenic MLL fusions, and is absolutely required for the fusions to cause leukemia. This project …
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Understanding Chemotaxis in the Nematode Caenorhabditis elegans: From Molecules to Behavior
… rGCs, all of which have the same basic domain architecture. I performed domain-swap experiments where the extracellular domain of one rGC was exchanged with the intracellular domain of another in all possible combinations. I was able to show that the extracellular domain is the region …
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Insights into p53-Dependent Apoptotic Signaling and Cell Fate vis-a-vis Functional Cooperation among BCL-xL, Cytoplasmic p53, and PUMA
… investigations of PUMA and the BCL‑xL×PUMA BH3 domain complex by X‑ray crystallography and nuclear magnetic resonance (NMR) spectroscopy reveal a novel, PUMA‑induced, domain‑swapped dimerization of BCL‑xL that requires a π‑stacking interaction between PUMA W71 and BCL‑xL H113. PUMA is an …