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Showing 1 to 8 of 8 for “"dnaB"”.
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Biochemical and Biophysical Characterization of Replicative Helicase Loading by <i>Vibrio cholerae</i> DciA, Crystal Structure of a Monoclonal Antibody Bound to a C-Terminal Fragment of EBNA-1 from Epstein Barr Virus
… loaders have outlined how the bacterial DnaB replicative helicase is loaded via mechanisms of “Ring-Breaking” by <em>E. coli </em>DnaC (EcDnaC)/protein P from bacteriophage lambda (LP) and “Ring-Making” by <em>B. subtilis</em> DnaI. Mounting evidence points to a recently discovered …
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Creating Genetic Engineering Tools for Investigating Bacillus anthracis.
… were identified by homology as targets. The <em>dnaB</em> gene, essential for <em>B. subtilis</em> initiation of DNA replication, was my focus. A vector system was created by polymerase chain reaction (PCR) with the pMUTIN4 integration vector and the promoter region of <em>dnaB</em> to study the …
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Mobile genetic elements in Coxiella burnetii: Friends, foes or just indifferent?
… interrupt the 23S rRNA gene; an intein within dnaB (encoding replicative DNA helicase) and a homing endonuclease. The introns are self-splicing ribozymes and able to inhibit ribosome function and retard bacterial growth through internal guide sequence (IGS)-dependent and -independent …
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Mobile genetic elements in Coxiella burnetii: Friends, foes or just indifferent?
… interrupt the 23S rRNA gene; an intein within dnaB (encoding replicative DNA helicase) and a homing endonuclease. The introns are self-splicing ribozymes and able to inhibit ribosome function and retard bacterial growth through internal guide sequence (IGS)-dependent and -independent …
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Uncovering vital molecular contacts within the replisome and characterizing fail-safe mechanisms ensuring genomic stability in E. coli.
… linker in the τ-subunit tethers α-Pol III to the DnaB helicase for efficient DNA replication in E. coli. Therefore, disrupting interactions between τ-CLC and α-Pol III also disrupts coordination with the DnaB helicase. To probe this phenomenon, we initiated our study through in vitro …
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Helicase-Initiated Assembly of Macromolecular Machines Involved in DNA Replication
… of as many as seven different proteins: PriA and DnaB helicases, DnaC, DnaG, DnaT, PriB, and PriC. Multiple and specific protein - protein, and protein - DNA interactions constitute integral part of this large molecular machine and dictate how this molecular motor assembles. However, the …
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Development of Bacillus subtilis spores and cells for surface display of proteins
… separated by a mini-intein derived from the SSp DnaB, which was then used as self-cleaving tag for purification by shifting the pH and/or temperature conditions, with no addition of any proteases or thiol reagent (Mathys et al., 1999). To construct the system, the mini-intein was fused downstream …