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Showing 1 to 14 of 14 for “"divisome"”.
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FtsK Regulates the Assembly of the Division Machinery in the FtsZ-less Chlamydia trachomatis
… including FtsZ, the central coordinator of divisome assembly. In the absence of FtsZ, we show that divisome assembly in Ct is initiated by FtsK, a chromosomal translocase. Chlamydial FtsK forms discrete foci at the septum and at the base of the progenitor mother cell, and our data indicate …
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Regulators of Bacterial Cell Division: Investigations of Min Oscillation and Ftsa Activity
… cytokinetic machinery in bacteria is termed the “divisome”. The divisome is a complex of proteins that forms a ring at midcell over dividing nucleoids. In order for daughter cells to form, the divisome must constrict. As constriction occurs, two new cell poles are formed, one for each daughter …
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The bacterial actin-like cell division protein FtsA forms antiparallel double filaments upon binding of FtsN
… of bacteria, cell division is carried out by the divisome, a multiprotein machine spanning the cell envelope. The divisome performs membrane invagination, peptidoglycan synthesis and remodelling, and eventually cell separation. Filaments of the tubulin homologue FtsZ form the scaffold for divisome …
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Characterization of Ftsa-Ftsn Interaction During Escherichia Coli Cell Division
… and constriction of the division machinery, or divisome. The <em>Escherichia coli </em>divisome includes nearly a dozen essential cell division proteins that assemble at midcell between segregating sister chromosomes. FtsZ, a homolog of eukaryotic tubulin, is the first essential cell division …
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Regulatory pathways controlling cell division after DNA damage in Caulobacter crescentus
… component of the cell division machinery or "divisome". Instead, SidA and DidA target the lateacting division proteins FtsW, FtsI, and FtsN to prevent divisome constriction, demonstrating that divisome components other than FtsZ can serve as regulatory targets. I have characterized mutations …
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Organization and Regulation of Proteins Required For Initiation of Cell Division In Escherichia Coli
… proteins to form the cell division machine, or “divisome.” FtsZ, a bacterial homolog of eukaryotic tubulin, forms a ring-like structure, the “Z ring,” at midcell and serves as a scaffold for divisome assembly and potential force generator during synthesis of the division septum. Both FtsA, a …
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Functional Analysis of FtsA-Mediated Recruitment of FtsK in Escherichia coli
… cytoplasmic and periplasmic components of the divisome to ensure that membrane constriction and chromosome segregation occur in synchrony. The actin-like protein FtsA plays a central role in this process by tethering FtsZ to the membrane and recruiting downstream division proteins. Despite its …
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Structure-function analysis of ZapC, an FtsZ-ring stabilizer, in Escherichia coli cytokinesis
… a multi-protein complex at midcell known as the divisome. Several divisome proteins promote the assembly/disassembly processes of FtsZ, thereby exercising spatiotemporal control over division. Among FtsZ regulatory proteins are the FtsZ ringassociated proteins (Zap), which either directly or …
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Mutations Within and Between Early Cell Division Proteins and Their Effects On Division Regulation In Escherichia Coli
… utilizes a macromolecular machine known as the divisome to accomplish cytokinesis. Assembly of the divisome begins with the assembly of a simpler structure known as the proto-ring. The proto-ring consists mainly of three essential proteins: FtsZ and its membrane tethers FtsA and ZipA. In this …
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Biochemical and structural studies of the FtsZ:FtsA complex and polymerising abilities of the FtsA protein
… and then recruits other components of the divisome, the cell division apparatus. Analysis of FtsA sequences revealed a conserved C-terminal motif, which is predicted to form an amphipathic helix and has been shown to localise FtsA (and with it FtsZ) to the membrane. FtsA belongs to the …
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PEPTIDOGLYCAN REMODELLING FACTORS: KEY PLAYERS IN CELL DIVISION AND HOMEOSTASIS MAINTENANCE OF ESCHERICHIA COLI
… periplasmic protein EnvC that is anchored to the divisome components FtsEX, and the outside of the cell thanks to the OM lipoproteins NlpD and ActS. In the first manuscript included in this thesis, it is shown the identification of the periplasmic deacetylase SddA, responsible for modifying the …
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Escherichia coli and Rhizobium leguminosarum response mechanisms to sub-lethal 2,4-dichlorophenoxyacetic acid
… by affecting the higher order assembly of the divisome complex with inhibition of Z-ring formation resulting from oxidative stress and DNA damage.
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PopZ and FtsZ coordinate polar growth termination and cell division in Agrobacterium tumefaciens
… additional proteins to mid cell to assemble the divisome, enabling activation of cell wall biogenesis to promote septum formation and cell separation. While further research is needed to understand how growth is targeted to the pole during elongation, our work provides mechanistic insights about …
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Characterizing the role of central carbon metabolism and cell wall stress responses in Bacillus subtilis cell wall synthesis
… elongasome (also termed as the Rod complex) and divisome, respectively. The synthesis of peptidoglycan is a dynamic process that is affected by various factors such as the availability of nutrients and external stresses. I have characterized the physiological role of an essential gluconeogenic …