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Showing 1 to 20 of 34 for “"dithiothreitol"”.
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Development and application of electrochemical dithiothreitol (DTT) assay for analysis of particulate matter
… an electrochemical version of the well-studied dithiothreitol (DTT) absorbance spectroscopy assay is presented as a platform for the analysis of PM in air samples. Flow injection amperometry was used as the primary electrochemical method. Amperometry was performed using a CH Instruments …
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Rethinking the dithiothreitol based PM oxidative potential: measuring DTT consumption versus ROS generation
… a specific ROS, hydroxyl radical (•OH) in dithiothreitol (DTT) assay. DTT assay has been a widely-used probe to measure the oxidative potential as it measures the consumption rate of DTT which presumably corresponds to the generation rate of a specific ROS - superoxide radicals (O2•-). …
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A comparison of sulfhydryl active compounds on activation and germination of Bacillus thuringensis spores
… lipoic acid and cystine (S-S), cysteine HCl and dithiothreitol (S-H). B^. thuringiensis spores incubated in cysteine HCl had a substantially lower germination rate compared to spores incubated only in Tris pH 7.5 buffer. \^en B^. thuringiensis spores incubated in cysteine HCl were washed, they …
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The Purification, Properties and Subunit Structure of Glycerol Dehydrogenase
… with the enzyme treated with urea and dithiothreitol, and with dithiothreitol and p- hydroxymercuribenzoate in the presence of guanidinium chloride indicated that the isolated enzyme is composed of subunits. The untreated enzyme, with a molecular weight of 3.4 x 10<sup>5</sup> daltons, …
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Modulation of calcium signalling in human sperm by nitric oxide
… (GSNO; an S-nitrosylating agent). Dithiothreitol application, to reduce protein –SNO groups, rapidly reversed the actions of NO and GSNO on [Ca\(^{2+}\)]\(_i\). The effects of NO, GSNO and dithiothreitol on protein S-nitrosylation, assessed using the biotin-switch assay, closely …
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Vascular endothelial cell-surface proteoglycans
… culture. Treatment of the parent molecules with dithiothreitol caused their complete cleavage and permitted the subsequent separation of the larger and smaller subunits on Sepharose CL4B columns. Removal of dithiothreitol by dialysis resulted in the reformation of large disulfide-bonded molecules …
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Biochemical studies on Oestradiol 17β in human mammary tumours and chemically induced rat mammary tumours
… binding in varying ways:mercaptoethanol and dithiothreitoL were stimulatory, L-cysteine was inhibitory. The dithiothreitol effect was further investigated with respect to anti-oestrogen inhibition and the effect of increased oxygen tension on binding was also studied, these results being …
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Cloning and characterization of neuraminidase from Salmonella typhimurium LT-2 and its relationship to neuraminidase from other bacteria
… to N-acetylneuraminic acid (NeuNAc) and dithiothreitol. Compared to many previously studied NANases, the S. typhimurium NANase is relatively unaffected by the competitive inhibitor, 2-deoxy-2, 3-dehydro-NeuNAc. NANase activity is maximal at a sodium chloride concentration of approximately …
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Conjugation of oligonucleotides for drug delivery via a linker derived from Meldrum’s acid and A capture-release method to synthesize long oligonucleotides
… aspect of this research is the substitution of dithiothreitol which is not naturally present in biological systems, with cysteine and glutathione as the biologically available decoupling agents. Our work optimized the conjugation reactions and release mechanisms, with the goal of developing a …
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Functional stability of a nucleic acid-hydrolyzing single chain antibody in various biochemical and biophysical environments
… retained in the presence of reducing agent, 2 mM dithiothreitol (DTT). Moreover, DNA-hydrolyzing activity of 3D8 scFv was maintained after lyophilization and 30 cycles of freeze-thaw. When the 3D8 scFv was stored in pH 3-12 for a month at 37°C, DNA-hydrolyzing activity was retained at pH 4-8. In …
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Hepatic 5'deiodinase activity of Japanese quail using reverse-T2 as substrate: assay validation, characterization, and developmental studies
… characteristics: maximal activity from 10-50 mM dithiothreitol (cofactor), an apparent Km of 0.52 µM rT3, pH optimum of 7.6-8.5, complete inhibition by 1 mM propylthiouracil and by 1 mM iopanoic acid, and substrate affinities of rT3 > T 4> T3 . Based on these characteristics, the quail hepatic …
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Gel-based proteomic comparison of two strains of Propionibacterium freudenreichii ssp. shermanii
… in the pH 4-6 region. Addition of 50 mM dithiothreitol (DTT) to the samples prior to isoelectric focusing (IEF) and an increase of IPG buffer concentration greatly improved spot resolution. Silver staining achieved higher spot counts than Coomassie staining. The influence of the number of …
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Cholesterol metabolism in the endoplasmic reticulum of rat liver
… purification of total cytochrome P-450 was low. Dithiothreitol, 4-phenyl-imidazole, diethyldithiocarbamate and glycerol, by themselves or in combination with each other, were shown to be useful agents in liver microsomal cytochrome P-450 purification. It was further demonstrated that increasing …
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Visible Light Cured Thiol-vinyl Hydrogels with Tunable Gelation and Degradation
… Specifically, thiol-containing molecules (e.g. dithiothreitol or cysteine-containing peptides) were used to replace TEA as both co-initiator and crosslinker. Co-monomer 1-vinyl-2-pyrrolidinone (NVP) was used to accelerate gelation kinetics. The gelation rate could be tuned by changing the …
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Extrathyroidal thyroid hormone activation in ring doves
… reverse triiodothyronine (rT3), cofactor (dithiothreitol) and trace amounts of I¹²⁵-rT3. 5’D activity was measured as I¹²⁵ released from the labelled rT3 during the assay incubation time. The 5’D assay was validated for all ages studied to assure that the initial reaction velocity of the …
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Cellular and acellular assays for measuring oxidative stress induced by ambient and laboratory-generated aerosol
… For chemical oxidative potential as measured by dithiothreitol consumption (OP), precursor identity influenced toxicity significantly, with isoprene and naphthalene SOA having the lowest and highest OP, respectively. Both precursor identity and formation conditions influenced ROS/RNS and cytokine …
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Historical Genetic Analysis Of Horned Lark (Eremophila Alpestris) Specimens From The Prairie Pothole Region
… column-based purification, chelating resin with dithiothreitol, and a Direct PCR method without DNA purification. Three fragments of mitochondrial DNA loci were amplified including control region, cytochrome c oxidase I and NADH dehydrogenase subunit 2. Correct sequencing results indicated that …
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Impact of heated passive headspace concentration on DNA recovery from semen in fire-scene evidence
… HPHC at 90 °C for 6 h or 16 h. The influences of dithiothreitol (DTT) pretreatment and gasoline contamination were also assessed. DNA yield, degradation index (DI) and short tandem repeat (STR) profiles served as outcome measures.When not contaminated with gasoline, about 60 ng of DNA (DI≈1.6) …
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Developing and Optimizing Conditions for Single Cell Genetic Analysis
… Liquid Nitrogen, 97% using Potassium hydroxide/dithiothreitol (KOH), 41% by boiling, and 85% for water. Allele drop-out was minimal in the KOH group, but was high in the water group. KOH lysis is superior in both sensitivity and specificity to other lysis methods tested. Nuclear and cytoplasmic …
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The Isolation and Characterization of Growth Regulatory Factors Produced by a Herpes Simplex Virus Type 2 Transformed Mouse Tumor Cell Line, H238
… trypsin, neuroaminidase, and the reducing agent dithiothreitol (DTT) and, resembled a platelet-derived growth factor (PDGF)-like factor; and the third displaced [ 125I]-labeled basic fibroblast growth factor (bFGF) in a dose dependent fashion when tested with a radioimmune assay (RIA). The fourth …
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