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Showing 1 to 20 of 44 for “"disk diffusion"”.
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Disk Diffusion Breakpoint Determination Using a Bayesian Nonparametric Variation of the Errors-in-Variables Model
<p>Drug dilution (MIC) and disk diffusion (DIA) are the two most common antimicrobial susceptibility tests used by hospitals and clinics to determine an unknown pathogen's susceptibility to various antibiotics. Both tests use breakpoints to classify the pathogen as either susceptible, …
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Comparison of Antibacterial Activity of Ozonated Olive Oil and Chlorhexidine Gluconate
<p>Aim: The aim of this in vitro disk diffusion susceptibility test is to compare the antibacterial activity of ozonated olive oil (OzOO) with chlorhexidine gluconate (CHX) against periodontal pathogens, Porphyromonas gingivalis and Aggregatibacter Actinomycetemcomitans. Materials and Methods: The …
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Identification of Inhibitory Compounds in Medicinal Mushrooms against L. monocytogenes and Z. bailii
… The inhibitory activity was measured using a disk diffusion assay and with optical density (OD). For OD, 7 fractions were collected using HPLC for 4 (A. blazei Murrill, G. lucidum, G. frondosa, I. obiquus) medicinal mushrooms and 6 fractions from L. edodes and 8 fractions from P. linteus. The …
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Does the human skin microbiome adapt to antibiotic exposure?
… their antibiotic susceptibility pattern (disc diffusion assay), biofilm formation (crystal violet microtitre plate assay) and microbial virulence (G. mellonella infection assay). Serially passaged antibiotic-adapted strains were also assessed for the following characteristics: colony and cell …
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Association of foodborne pathogens with Capsicum annuum fruit and evaluation of the fruit for antimicrobial compounds
… peppers to test for antimicrobial activity. A disk diffusion assay determined that the extracts were capable of inhibiting the growth of the pathogenic bacteria tested. Listeria monocytogenes was especially sensitive to the extracts. jalapeño extracts were fractionated using HPLC and used for …
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ΣΤΕΛΕΧΗ STAPHILOCOCCUS AUREUS ΑΝΘΕΚΤΙΚΑ ΚΑΙ ΕΥΑΙΣΘΗΤΑ ΣΤΗ METHICILLIN: ΛΥΣΙΤΥΠΙΑ, ΑΝΙΧΝΕΥΣΗ ΠΛΑΣΜΙΔΙΩΝ ΚΑΙ ΠΡΩΤΕΙΝΩΝ ΚΥΤΤΑΡΙΚΗΣ ΜΕΜΒΡΑΝΗΣ
… TO A BATTERY OF ANTIMICROBIAL AGENTS BY DISK DIFFUSION AND M/C DETERMINATION. FI ISOLATES WERE LINCOMYCIN AND METHICILLIN RESISTANT (LMRSA). IN THE GROUP OF 55 LMRSA ISOLATES, 13 WERE ALSO RESISTANT TO VANCOMYCIN. ALL THE LMRSA ISOLATES WERE NOT TYPED BY THE INTERNATIONAL SET AND THE …
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Investigation of the prevalence of antibiotic resistant bacteria and their genes in wastewater
… Resistance capabilities were detected using the disk diffusion method and resistance isolates were identified using the 16S rDNA sequencing. Polymerase chain reaction (PCR) with specific primers was used to detect resistant genes. Penicillin, ampicillin, meropenem and imipenem traces were …
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Genetically engineered phage fibers and coatings for antibacterial applications
… determined via live-dead staining and a modified disk-diffusion (Kirby-Bauer) measure of zone of inhibition (Zol) against Staphylococcus epidermidis and Escherichia coli bacterial strains. Live-dead staining and Zol distance measurements indicate increased bactericidal activity in the genetically …
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Assessment of antibiotic resistance phenotypic patern in some commensal bacteria isolated from meat and dairy products
… susceptibility testing was performed by disk diffusion method on Mueller-Hinton agar according to the Clinical Laboratory Standards Institute (2007) standards. A total of twenty eight (28) antibiotics were used to determine the antibiotic susceptibility of commensals which include eight …
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Similaridade genotípica de Salmonella enterica isolada de animais silvestres e exóticos em cativeiro em Ohio, EUA
… to antimicrobial Susceptibility testing by disk diffusion method, serotyping and genotyping by macrorestriction of chromosomal DNA. Salmonella was detected in 56/225 (25%) of fecal samples, 6/56 (11%) of environmental samples and 6/38 (16%) of food samples. This pathogen was more frequently …
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Using wildlife to biomonitor for antimicrobial resistance in the Scottish environment
… breakpoint plate (sample level) and published disk-diffusion (isolate level) methods, we found that resistance to tetracycline was the most prevalent phenotype, while resistance to other antimicrobial classes was generally low or not detectable. However, instances of clinical resistance to …
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Macrolide and lincosamide resistance patterns in invasive and colonizing group B streptococcus isolated in Belgium
… to clindamycin were assessed by a double-disk diffusion test. The presence of genes encoding RNA methylases (erm) and efflux pumps (mef) was confirmed by PCR. Of the 328 GBS isolates, 109 (33,2%) were resistant to erythromycin. Among these isolates, 102 (93,6%) exhibited the MLS phenotype …
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Genotyping antibiotic resistance properties of escherichia coli and campylobacter jejuni associated with diarrhoea in young children in the Vhembe District
… around the Vhembe District. The Kirby Bauer Disk Diffusion technique was used to screen for antibiotic susceptibility, and PCR and sequencing were used for molecular characterization of antibiotic resistance genes. Results: Of the E. coli positive samples, 39% (18/46; 12 diarrhoeal and 6 …
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Evaluation of anti-tuberculosis potentials of selected medicinal plants in Endau Rompin, Johor, Malaysia
… for their antimycobacterial activity using agar disk diffusion assay, Tetrazolium Microplate Assay and agar plate assay against Mycobacterium smegmatis. The effect of the extract on mycobacterial cell at the cellular level was investigated upon treatment with the crude extracts via time-kill …
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Optimization of a Method for Testing Ballast Water for Enterococci and an Investigation on the Occurrence of Antibiotic Resistance in Vibrio cholerae
… fourth- generation cephalosporins were tested by disk diffusion. Genotypic analysis of the resistance determinants was performed by PCR to detect ESBL (blaCTX, blaTEM, blaSHV), carbapenemases (blaIMP, blaSPM, blaVIM, blaBIC, blaNDM, blaKPC, blaAIM, blaSIM, blaDIM, and blaGIM). All strains were …
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Antimicrobial susceptibility of Acinetobacter isolates from Groote Schuur Hospital region : an investigation of the appropriateness/validity of the NCCLS zone size criteria
… of infections caused by Acinetobacter.The disk diffusion method is the technique most widely used by microbiology laboratories for the routine assessment of antimicrobial susceptibility patterns.The zone diameters obtained by this technique for individual antimicrobials are reported as …
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Assessing bacteriological quality of fresh black soldier fly larvae.
… Susceptibility Test (AST) using the Kirby-Bauer disk diffusion method. Antibiotics-resistant isolates were further assessed for extended-spectrum beta-lactamase-encoding genes using Polymerase Chain Reaction. From 17 samples the process of culturing yielded 79 isolates based on colony morphology. …
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Assessing bacteriological quality of fresh black soldier fly larvae.
… Susceptibility Test (AST) using the Kirby-Bauer disk diffusion method. Antibiotics-resistant isolates were further assessed for extended-spectrum beta-lactamase-encoding genes using Polymerase Chain Reaction. From 17 samples the process of culturing yielded 79 isolates based on colony morphology. …
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Prevalence, virulence factors and antimicrobial resistance of salmonella species from beef in retail outlets from KwaZulu-Natal province, South Africa
… Minorscheme. The KirbyBauer disk diffusion method was used to determine antimicrobial resistance profiles of the Salmonellaisolates against Cefotaxime, Kanamycin, Ampicillin, Amoxicillin, Trimethoprim-Sulfamethoxazole, Ciprofloxacin, Chloramphenicol, Gentamicin, Cefoxitinand …
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The screening of medicinal plants traditionally used to treat diarrhoea, in Ongoye area, KwaZulu Natal.
… The antibacterial activities were determined by disk-diffusion, agar-well diffusion, minimum inhibitory concentrations (MIC) and bio-autographic methods. The plant extracts were screened for the following phytochemrcals: alkaloids, flavonoids, soponins, anthraquinones, cardiac glycosides and …
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