Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 60 for “"dimerisation"”.
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Towards Optimising Lentiviral Vectors Through Structure Informed Genome Modification
… RNA that become linked via a process known as dimerisation. I focused on improving the infectivity of vectors by targeting their dimerisation and packaging properties based on the hypothesis that WT HIV-1 regulates genome encapsidation tightly by recognising dimeric RNA. The genomic RNA (gRNA) …
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An optical proteomic profile of the HER2 family in breast tumours and the effect of exposure to tyrosine kinase inhibitors
… inevitably develops to both drugs. HER2:HER3 dimerisation is vital to the translation of extracellular signals, via intracellular signaling cascades, to promote cancer cell proliferation, invasion and metastasis, and is studied herein. Regulation of the amplitude and kinetics of signal …
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MEK1 binds βarrestin1 directly, influencing both its phosphorylation by ERK and the timing of its isoprenaline-stimulated internalization.
… in both yeast and mammalian cell lines. (2) The dimerisation requires multiple interaction sites such as R173/N174/N175, E228/T229/L230, L306/M307/H308 and K323/T324/E325 (together named QUAD) in which R173/N174/N175 contributes most toward the dimerisation. (3) Association of an ion-pair …
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Exploring the oligomeric state of TLR4 and downstream regulators RIPK1 and RIPK3
… repeats that recognise ligands, leading to the dimerisation of their intracellular Toll/interleukin 1 receptor (TIR) domain, which builds a platform for signalling via the MyD88- and TRIF-pathway. TLR4, with its co-receptor MD2, unlike the other TLRs, can signal via the MyD88- and TRIF-pathway …
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Total Synthesis and Stereochemical Revision of Pestalospirane B
… pestalospirane cores A (275a) and B (275b) via dimerisation of synthetic monomer 273. These investigations established a sound platform from which a total synthesis of the natural product pestalospirane B (3) could be achieved. The second part of this thesis describes considerable effort focused …
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Characterising the effects of ACK-mediated phosphorylation on the Class IA PI3-Kinase regulatory isoforms
… to promote cell proliferation. Since protein dimerisation is a well-established mechanism for modulating protein function, it is possible that phosphorylation-driven dimerisation of the p85 isoforms could represent a novel mechanism for regulating PI3-Kinase signalling. Accordingly, this …
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The structure of the cytoplasmic dynein tail
… important for both cargo binding as well as homodimerisation of the DHC, which is necessary for processive movement. Previous studies suggest that the DLCs play an important role in homodimerisation, but it remains unclear how else the DHCs are held together. Using S. cerevisiae as a model …
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Novel Catholyte and Electrolyte Optimisation for Aqueous Organic Redox Flow Batteries
… Evidence suggests that this is the result of dimerisation of AA-based redox species. An extensive study using electrochemical impedance spectroscopy (EIS) showed that NA prevents a thick, charge-transfer blocking, film from forming on interphases in the cell (i.e. the electrode or membrane), …
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Investigating the Role of Stem-Loop 1 in the Assembly Process of HIV-1
… (gRNA) delay CA-SP1 processing. SL1 harbours the dimerisation initiation site (DIS) palindrome suggesting that efficient Gag processing may be linked to gRNA dimerization as shown in HIV-2. However, a dimerisation mutant with normal Gag processing was identified. Gag processing defects are …
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Multinuclear NMR Investigations of Local Structure, Distortions and Redox Mechanisms in Layered Lithium Ion Battery Cathode Materials
… the Ru^{4+} ions form dimers and the effect of dimerisation on the observed ^{17}O NMR shifts is elucidated. The dimers, which are ordered at room temperature, lose their long-range ordering above 260 °C when Li_2RuO_3 undergoes a phase transition. This phase transition is characterised using …
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Functional studies on receptor-type protein tyrosine phosphatases of the R3 subgroup
… defined. Potential roles in ligand interaction, dimerisation and cell-cell contacts have been reported. Here I used a bimolecular fluorescence complementation (BiFC) assay in live cells to examine the molecular basis for the interaction of one of the R3 RPTP members, VE-PTP, with VE-cadherin, and …
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Mechanisms of piRNA-guided co-transcriptional gene silencing
… dynein, Ctp has been shown to function as a dimerisation hub protein, mediating the assembly and stabilisation of many dynein-independent protein complexes. I demonstrated that the primary function of Ctp within PICTS is to support the assembly of a higher-order complex through dimerisation …
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The chemistry of 1,2-dioxines.
… of the cyclopentenes are discussed. A novel dimerisation of cis-γ-hydroxyenones catalysed by lithium hydroxide was found giving rise to highly substituted tetrahydrofurans. Both l,2-dioxines and trans-γ-hydroxyenones could be used as starting materials for the dimerisation. The reaction …
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Investigating K-Ras inhibition by α-helical peptides
… were validated biochemically, however their dimerisation prevented K-Ras binding. A point mutation successfully reversed dimerisation, although K-Ras binding of the resulting RBD could not be verified. Rational design of RLIP76 RBD point mutations enabled K-Ras binding in a single case, …
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Regulation of inflammatory TLR signalling by the BCAP adaptor
… revealed that the DBB domain functions as a dimerisation region. First shown in NF-κB reporter assays, DBB domain dimerisation also prevents MAL TIR domain interactions in vitro. These results support a new model of steric inhibition of TIR domain interactions, by which BCAP negatively …
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Zur Palladium-katalysierten Dimerisierung von Acrylsäuremethylester
… the optimisation of the palladium catalysed dimerisation reaction of methyle acrylate by using mono- and bidentate ligands and ionic liquids as solvent. Furthermore a biphasic reaction system is developed and applied to a continuos reactor. The activity of the Palladium catalyst increases …
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Nucleic Acid Scaffold-dependent Proximity-mediated Enzyme Response (NASPER) - A Proof of Concept Study
… and the split-TEV protease, which require dimerisation/fragment complementation for catalytic activity. In Chapter 3, the two designed PUF proteins were first purified and tested in vitro using fluorescence polarisation experiments to assess RNA binding. The results indicate that the PUF …
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Investigating the Role of Oncogenic KRAS G12 Mutations in Cell Signalling
… most likely a splice variant that has enhanced dimerisation capabilities with CRAF. RAF dimerisation is one mechanism via which RAF inhibitors fail to be effective in treating KRAS mutant cancers. This exemplifies the importance of characterising the identity and role of this BRAF form, as it …
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The Development of Photo-crosslinkable Trapping Mutants as Tools to Investigate the Interactions of Protein Tyrosine Phosphatases
… the photo-cross-linkable F68pBPA variant of VHR, dimerisation of<br/>VHR was detected in an in vitro ultraviolet (UV) exposure-mediated crosslinking<br/>assay. VHR dimerisation was further demonstrated to be a potential<br/>novel regulatory mechanism for VHR, having a negative effect on the …
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The role of adaptor protein Mal/TIRAP in transducing TLR signalling
… or preformed dimers, and no ligand induced dimerisation occurs in response to LPS. Mal/TIRAP was pre-associated with TLR4 and TLR2 in both naïve and activated states. The stoichiometry and association of Mal/TIRAP with TLRs remained unchanged upon ligand stimulation, suggesting that signal …
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