Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 38 for “"digital PCR"”.
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The Effects of Known PCR Inhibitors on Droplet Digital PCR Performance
… that can inhibit the polymerase chain reaction (PCR). Inhibitors associated with forensic samples include substances that are endogenous with the sample (calcium, melanin), absorbed from the environment (humic acid, indigo dye)1, or introduced during laboratory processing (phenol1 , glove …
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Droplet digital PCR detection and next-generation sequencing of HIV-1 in breastmilk of breastfeeding women on antiretroviral treatment
… DNA quantitation in breastmilk using droplet digital PCR (ddPCR) and sequencing of viral variants in breastmilk using Illumina MiSeq next generation sequencing to inform transmission risk and efficacy of maternal ART clinical trials. HIV-negative breastmilk samples were spiked with 8E5 cells …
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Evaluation of Digital PCR (dPCR) for the Quantification of Soil Nitrogen Turnover Bacteria in Wetland Mesocosms in Response to Season, Fertilization, and Plant Species Richness
… was investigated. The chip-based QuantStudio 3D digital PCR (QS3D dPCR) system was used to quantify ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB), comammox, anammox, and denitrifiers. Principal component analysis (PCA) was used to identify dominant patterns in the microbial …
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Bartonella in Small Mammals from Montana and Idaho
… prevalence of approximately 37% based on droplet digital PCR (ddPCR) screening. Following ddPCR screening, individuals that yielded positive results were selected for further analysis including genetic sequencing of the RNA polymerase b-subunit (<em>rpoB</em>) gene. While 116 individuals of 313 …
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Antibiotic Resistance Genes in Marquette County Michigan's Wastewater
… resistance profiles. A Qiagen Microbial DNA qPCR plate screened for 87 different ARGs and detected 41, most encoding beta-lactam resistance. Ten ARGs were quantified using droplet digital PCR: bla<sub>CTX-M</sub>, bla<sub>TEM</sub>, bla<sub>CMY-2</sub>, bla<sub>SHV</sub>, KPC, NDM, OXA-48, …
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Evaluating Determinants of Mitochondrial Mutagenesis and their Intersections with Cell Fate
… on mtDNA frequency with newly developed droplet digital PCR assays. Here I found that these classic nuclear mutagens damage mtDNA, but are not capable of significant mtDNA mutation induction. Next, I evaluated the cellular effects resulting from expression of a mitochondrial-targeted form of the …
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Identificazione e Validazione di Hotspot di Metilazione del DNA come Biomarcatori per il Melanoma Cutaneo
… RARB and ISL1 were evaluated by using droplet digital PCR (ddPCR) while the methylation levels of the DNA methylation hotspots computationally identified were assessed using a custom protocol developed by the Laboratory of Experimental Oncology of the University of Catania defined Methylation …
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Structural variation at soybean loci regulating small RNAs and seed color
… resequencing and copy number determination by digital PCR. Two size deletions (130 kb and 22 kb) were discovered that each eliminate part of the 27-kb inverted repeat CHS cluster resulting in black seed coats. This study demonstrates the importance of the correct representation of the target …
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The development of a blood-based test of DNA methylation for the earlier detection of high-grade serous tubo-ovarian cancer
… analysis, probe-based quantitative PCR and digital PCR were employed to analyse a selection of candidate DNAme markers. The most promising DNAme marker was analysed in a cohort of matched tissue and plasma samples using an optimised methylation-sensitive restriction enzyme PCR (MSRE …
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Advancing detection, genomics, and secretome analysis of oomycete pathogens Phytophthora capsici and Phytopythium vexans to explore mechanisms of pathogenesis and biocontrol solutions
… of an amplitude-based multiplex droplet digital PCR (ddPCR) assay. Designed for simultaneous quantification of P. capsici, Trichoderma asperellum, and Trichoderma gamsii in soil, this assay was validated in field trials and offers high sensitivity and specificity. The assay displayed a …
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Mapping pathogen distributions and population connectivity of a sentinel Arctic species, the polar bear (Ursus maritimus) across a changing North American Arctic
… a sensitive multiplexed, magnetic-capture, and digital PCR tool for surveillance of five zoonotic pathogens (three bacteria Erysipelothrix rhusiopathiae, Francisella tularensis, and Mycobacterium tuberculosis complex (MTBC), and two parasites T. gondii and Trichinella spp.) relevant to wildlife …
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Investigating the heritability of resistance fueling copy number variation in glyphosate resistant Amaranthus palmeri
… estimates were obtained by probe-based droplet digital PCR and the copy number estimates were used to perform controlled crosses to create the F1 generation. The results illustrate an overall increase in mean copy number in response to increasing mean EPSPS copies in the parents. I report a …
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Expression Analysis and Stem Cell Engineering
… developed and evaluated. In the first study, a digital PCR technology, called polonies, was applied to measure mRNA from several key stem cell genes in small numbers of ES cells. Due to its properties, we hypothesized that polonies would be uniquely poised to profile stem cells. Polonies were …
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Monitoring trace levels of ctDNA using integration of variant reads
… to 16% in a similar cohort analysed with digital PCR. In addition, INVAR may enhance detection of ctDNA in samples with limited input or sequencing coverage by aggregating signal across a large number of mutations. Using low-depth WGS (0.6x), ctDNA was detected to 1 mutant per 10,000 …
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Cell-free fetal DNA (cffDNA) enrichment for non-invasive prenatal testing (NIPT): a comparison of molecular techniques
… temperature polymerase chain reaction (COLD-PCR), inverse-PCR and Pippin Prep™ gel electrophoresis, were analysed to identify a novel approach to selectively enrich shorter cffDNA fragments from larger maternal cell-free DNA (cfDNA). The sensitivity of droplet digital PCR (ddPCR) against …
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Investigating the role of circulating cell-free dna as a mechanistic biomarker in inflammatory bowel disease: development of an integrated precision-medicine enabled platform in Scotland
… out using Qubit for total quantification, digital polymerase chain reaction (dPCR) for COX3, ND2 and GAPDH genes, fragment analysis with the Agilent BioAnalyzer, and cfDNA sequencing using both Nanopore and Illumina platforms. RESULTS: Patients with highly active IBD (requiring admission to …
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Biomarcatori circolanti per ictus in pazienti con cancro: studio pilota
… e amplificazione basato su un sistema droplet digital PCR (ddPCR) ad alta sensibilità. I dati prodotti sono stati analizzati considerando le caratteristiche cliniche e patologiche dei pazienti per identificare nuovi efficaci biomarcatori epigenetici associati a differenti quadri clinici. …
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microRNAs in Uveal Melanoma: in vivo findings and computational analysis
… of the three miRNAs under investigation. Droplet digital PCR (ddPCR) was used for both liquid biopsy and tissue samples. A novel computational analysis for the three selected miRNAs was performed in order to corroborate the experimental results. Results: Significantly higher expression levels of …
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The microbiology of chronic suppurative otitis media
… of clinically relevant bacteria. Droplet digital PCR (ddPCR), amplicon sequencing and multiplex PCR were utilised in this study. The distribution of the bacteria within the tissue was examined using histology and Gram staining. Genera Staphylococcus and Pseudomonas, the main pathogens …
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