Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"diffusional sizing"”.
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Microfluidic diffusional sizing at the single molecule level
… presents the single-molecule microfluidic diffusional sizing (sm-MDS) platform and demonstrates its capabilities at performing diffusional sizing at picomolar sensitivity. Microfluidic diffusional sizing (MDS) is a robust, in-solution method to measure hydrody- namic radii (Rhyd) of …
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Physico-Chemical Characterisation of Disease-Related Protein-Protein Interactions in Solution
… under physiological conditions. Microfluidic diffusional sizing offers an alternative method for investigating protein-protein interactions and protein assembly under physiological conditions and has been applied to investigate a wide range of protein properties, functions, and interactions. …
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Development of Original and Notable Techniques for Protein Analysis, Native Identification, and Characterisation
… particles using capillary electrophoresis and diffusional sizing. This allowed to characterise the components of heterogenous solutions in function of their size and the mobility of the proteins. The third chapter is looking at existing well-known microfluidic techniques, like free-flow …
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Investigating Protein Properties via Microfluidic Techniques
… Chapter 2 demonstrates that a microfluidic diffusional sizing device can be employed to study protein interactions with small molecules by measuring the variation of hydrody- namic radius of bovine serum albumin (BSA) in aqueous solution as a function of pH. By simulating the behaviour of …
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Advances in biophysical methods for protein detection and characterisation
… a time correlation technique is used for local sizing on chip. Second, microfluidic techniques are routinely used to create protein assays. These assays minimise the amount of sample required and the absence of turbulences enable new techniques. A method to easily add nanofluidics elements to …
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Microfluidic Approaches for Investigating Aggregated Forms of Disease-Related Proteins
… this thesis I describe the development of Immuno Diffusional Sizing (IDS), a method that combines diffusional sizing with the sensitivity and specificity of immunoassay detection. In Chapter 3, I explain the optimisation of the devices developed to size a range of particle sizes in heterogeneous …
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Characterisation of Alloantibody-Human Leukocyte Antigen Interactions to Improve Immunological Risk Assessment in Solid Organ Transplantation
… Lastly, using the principles of microfluidic diffusional sizing and Bayesian inference, microfluidic antibody affinity profiling (MAAP) was developed to enable in-solution, simultaneous determination of the KD and [Ab] of antibodies directly in patient serum samples. Quantification of purified …
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Advances in understanding protein-lipid interactions through biophysical and bioelectronic approaches
… transmission electron microscopy, microfluidic diffusional sizing and confocal microscopy. In our studies, we have aimed to incorporate natural membrane lipids in addition to the conventionally used synthetic lipids in order to increase the biological significance of our findings. We have also …
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Biochemical and Biophysical Studies of the UPR Kinase PERK and its Interactors in Health and Disease
… and biophysical methods, namely microfluidic diffusional sizing and Förster resonance energy transfer (FRET), to investigate interactions of PERK in solution and live cells. In-solution interaction studies confirmed recruitment of eIF2α by the unique insert loop situated within PERK’s kinase …