Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 52 for “"differentiation potential"”.
-
Phenotypic analysis of human stem cells and their differentiation potential towards pancreatic cells
… insulin-producing cells. The published cell differentiation protocols were to be validated, and novel ways of improving upon the protocols were to be examined with the ultimate aim to produce cells that closely resembled pancreatic β-cells. In this study, the above stem cell populations were …
-
Trophoblast specification and differentiation potential in early human and non-human primate embryogenesis
… trophoblast specification and (2) trophoblast differentiation, and ultimately the conservation of these processes in human. To delineate the molecular framework of marmoset trophoblast development, a single-cell RNA sequencing dataset was generated from zygote to postimplantation embryos at …
-
The mechanical characteristics and differentiation potential of the axial progenitor region in vertebrate embryos
… and evaluated how disrupting it influences the differentiation potential of NMCs. Firstly, I measured the PZ and surrounding tissues using atomic force microscopy (AFM). I showed that the notochord, the NMCs, and the PSM are distinct mechanically; with the notochord being the stiffest and the …
-
In vivo analysis of homing pattern and differentiation potential of cells deriving from embryonic and adult haematopoietic regions
… fact that different cell lines have different potentials in developing embryos, dependent on their cellular identity. The cell lines used in this survey were AGM region-deriving DAS 104-4, DAS 104-8 cells, yolk sac-deriving YSE cells and bone marrow-deriving FDCP mix cells. These cells were …
-
Evaluation of Myogenic Differentiation Potential of BMD iPSCs in vitro and the Effect of Ubiquitination Inhibitors on Dystrophin Stability in vivo
… iPSCs in tissue regeneration also serves as a potential therapeutic strategy. This study aims to test the efficacy of selected drugs interfering with the dystrophin polyubiquitination using BMD iPSCs as the model system. The hypothesis is that myogenic differentiation of the BMD iPSC-derived …
-
ADULT HUMAN BRAIN MENINGES HOST NEURAL STEM/PRECURSOR CELLS THAT CAN BE IN VITRO EXPANDED AND SHOW MULTIPOTENT NEURAL DIFFERENTIATION POTENTIAL IN 2D AND 3D CULTURE
… for the NSCs survival, proliferation and neural differentiation. However, in adult human brain the presence of active NSC niches and the generation of new neurons after birth is still debatable [3]. Recent data highlighted that meninges, the protective coverings of the brain and spinal cord, are …
-
ESTABLISHMENT IN CULTURE OF MOUSE AND HUMAN STEM CELLS WITH EXPANDED FATE POTENTIAL
… of a stem cell state that possessed expanded differentiation potential to the embryonic and both extra-embryonic lineages in vitro and in vivo. These cells were isolated directly from in vitro culture of mouse pre-implantation embryos or single cell blastomeres, reprogrammed from somatic cells …
-
Bionanoparticles as Viable Substrates to Promote Osteogenic Differentiation of Bone Marrow Stromal Cells
<p>Bone marrow stromal cells (BMSCs) have the potential to differentiate into osteoblasts, chondrocytes, adipocytes and smooth muscles. Although, they have shown great prospects in therapeutic and medical applications, less is known about the role that the nano environment plays on their …
-
Characterization and application of adipose derived stem cells following burn injury
… in adipose-derived stem cells (ASCs), which hold potential for healing burn wounds or generating skin substitutes. Using a 60% rat scald burn model, we investigated the cellular source of inflammation within adipose tissue and whether inflammation affects ASC fate and function. Endpoints included …
-
Characterization and comparison of cell frequency, growth, and multipotential differentiation of adult mesenchymal stromal cells derived from equine bone marrow and adipose tissue
… tissues, cell doubling characteristics and differentiation multipotential in culture based on histochemical staining and compositional analysis. Equine MSCs and ASCs from young adult horses were harvested and isolated from sternal bone marrow and supragluteal subcutaneous adipose tissue, …
-
Toward understanding and mitigating heterogeneity in bone marrow stromal cell cultures for improved therapeutic efficacy
… community not only for their demonstrated differentiation potential toward several lineages, but also due to the anti-inflammatory and immunomodulatory effects of their secretome. However, the necessary in vitro expansion of BMSCs renders cell populations functionally diverse, and …
-
Isolation, Culture and Cryopreservaion of Neural Stem Cell
… (-) cells could generate neurospheres but the differentiation potential into oligodendrocyte was reduced in eGFP(-) neurospheres. To determine differentiation ability neurosphere from single cell, we selected by clonal selection and cultured for 10 day. Single cell was formed neurosphere and …
-
Characterization of Genetically Modified Mesenchymal Stem Cells with a Retroviral Vector Expressing a Suicide Gene
… to passage 11. We found that proliferative and differentiation potential, chromosomal stability and surface antigenicity of mesenchymal stem cells were not altered by retroviral transduction. The results indicate that retroviral vectors can be safely utilized for deliver suicide genes to …
-
Role of Wnt signaling in proliferation of human bone marrow-derived mesenchymal stem cells
… application. However, their proliferation and differentiation potential were gradually decreased during in vitro expansion. Wingless-related MMTV integration site (Wnt) is well known signaling molecules that implicated in mesenchymal differentiation during development, but the role in …
-
Differentiation plasticity of stromal vascular fraction cells towards adipose tissue and endothelial structures
… between adipose tissue and endo-thelium, and the differentiation potential of cells to convert from one tissue into the other via progenitor cells, have not been elucidated so far and are therefore the focus of this thesis. Stromal vascular fraction (SVF) cells were isolated from liposuction …
-
Osteogenic differentiation of human mesenchymal stem cells on thin film tyrosine derived polycarbonates
… of regenerative medicine because of the vast differentiation potential into various cell lines such as: osteoblasts, chondrocytes, adipocytes, and neurons. Osteogenic differentiation of human mesenchymal stem cells (hMSC) could be an important tool in the treatment of orthopedic deficiencies …
-
The role of E2f4 in cell cycle exit and bone development
… As cell cycle exit is often concomitant with differentiation, it is not surprising that mutation of the E2Fs and pocket proteins results in defective development and differentiation. Mutation of the most abundant E2F, E2F4, is known to disrupt the proper differentiation of several cell types, …
-
Dreidimensionales epidermis-ähnliches Wachstum humaner mesenchymaler Stammzellen auf dermalen Äquivalenten : Einfluss der experimentellen Kulturbedingungen auf Differenzierung, Migration und funktionelles Verhalten
… cell-based therapies. This study explores the differentiation potential of hMSC in a tissue-specific microenvironment simulated in vitro. Human MSC were cultured air-exposed on dermal equivalents (DEs) consisting of collagen types I and III with dermal fibroblasts and subjected to similar …
-
Functional analysis of the histone variant H2A.Z during lineage commitment
… We found that this modification regulates the differentiation potential of mouse embryonic stem cells (mESCs). Loss of H2A.Z ubiquitylation leads to a disrupted chromatin state and derepression of key developmental regulators in mESCs. Furthermore, we show that H2A.Zub is a crucial component …
-
Identification of Unique Hematopoietic Stem Cell Properties
… suggested that HSCs might possess a much broader differentiation potential but in most cases the reported efficiency of such developmental plasticity has been rather low. We demonstrate that HSCs efficiently engraft within the infarcted myocardium following transplantation. However, engraftment is …
Page 1 of 3