Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"differential protein"”.
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Differential Protein Expression and Butanol Production using <i>Clostridium beijerinckii</i>
… bacterial proteome in efforts to identifying key proteins that play role in the overall process of butanol fermentation. In the future we hope to genetically engineer microorganisms to efficiently carry out butanol fermentation at commercially significant levels. We were also able to find that the …
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Gel-based proteomic comparison of two strains of Propionibacterium freudenreichii ssp. shermanii
… of P. freudenreichii and detect inter-strain differential protein expression. The influence of a number of parameters on the quality of 2-D gels was also investigated. The pH 3-7 and pH 3-11 NL proteome maps obtained displayed 305 and 356 spots, respectively, and detected the differential …
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Quantitative Approaches for Protein Differential Expression Analysis
… protocols were developed to facilitate differential protein expression analysis and biomarker discovery via a two-step sample interrogation strategy: (a) global protein profiling and differential expression analysis by spectral counting; and, (b) biomarker candidate validation by …
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Overexpression of active AKT3 induces differential binding of coregulator proteins to the estrogen receptor as a possible mechanism of Tamoxifen resistance
… attributed to binding of different co-regulator proteins and/or different binding affinity of these proteins to the estrogen receptor in M CF-7 cells overexpressing active Akt3 as compared to parental MCF-7 cells. We have immuno-precipitated the estrogen receptor along with bound co-regulator …
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The characterization of cysteine protease 4 and superoxide dismutase 6 in Trichomonas vaginalis
… expression of CP4. T. vaginalis strains showed differential protein and mRNA expression, although it was unclear which CP4 variants (other than TVAG_355480 and TVAG_467970) were recognized by the CP4 antibody for the protein studies. Iron did not regulate expression of TVAG_355480 and …
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Proteomics characterization of HPV-independent Vulvar Cancer Tumour Progression and Metastasis by Mass Spectrometry
… to the low-risk HPV-dependent type, whereas protein studies focused on the HPV-independent type are quite limited. The protein biomarker is an important diagnostic tool in cancer early detections and metastasis predictions. However, there is no protein biomarker targeting on HPV-independent …
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Precision Medicine in Immunotherapy and Lung Cancer
… mononuclear cells (PBMCs) and identified key differentially expressed genes of interest. A deeper analysis of associated single nucleotide polymorphisms (SNPs) affecting specific gene expression led to the development of a predictive polygenic risk score for the onset of immunotherapy-related …
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Proteomic identification of putative biomarkers of neo-adjuvant chemotherapy resistance in luminal (ER+) breast cancer
… were performed using ductal ER+ tumours. Differential protein expression was compared between chemoresistant and chemosensitive samples using the Panorama XPRESS Profiler725 AbMA kit. The combined data from 9 AbMA assays and 3 2D-PAGE/MS experiments was then analysed using Ingenuity …
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Hypothalamic Transcriptional Profiling and Quantitative Proteomics of Mice under 24-Hour Fasting
… quantitative proteomics were used to identify differential protein levels during fasting, and in a genetically obese mouse model, Mice were given either a 24-hour fast, or ad libitum access to food. Hypothalamic RNA and microRNA samples were analyzed by microarray, using both the Affymetrix and …
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Proteomic Map of ER+ Breast Cancer Cell Cycle
… mechanisms, molecular pathways and specific proteins involved in cell cycle progression in ER+ breast cancer cells. We focused on the G1-to-S phase transition of the cell cycle because major differences in cell proliferation mechanisms between normal and cancerous cells are observed at this …
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Differential expression profiling of proteomes of pathogenic and commensal strains of Staphylococcus aureus using SILAC
… by this pathogen. We proposed the variation in protein expression by commensal and pathogenic strain as an important factor behind the difference in pathogenicity. The identification of differentially expressed proteins was carried out using a quantitative mass spectrometry (MS)-based proteomic …
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Biomarker Discovery in Autoimmune Diseases: A Proteomics Approach
Proteins constitute the functional machinery of cells and are prime candidates for disease marker discovery. Mass spectrometry-based proteomics biomarker discovery holds the ability to interrogate a constellation of proteins simultaneously in a high-throughput manner to uncover a panel of markers …
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Identification of differentially expressed genes in AHI-1-mediated leukemic transformation in cutaneous t-cell lymphoma
… fungoides. Aberrant expression of AHI-1 mRNA and protein has been found in CD4⁺CD7⁻ leukemic Sezary cells from patients with Sezary syndrome. Moreover, stable suppression of AHI-1 using retroviral-mediated RNA interference in Hut 78 cells inhibits their transforming activity in vitro and in vivo. …
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Towards an understanding of the developmental zebrafish (Danio rerio) proteome and impacts of age-specific sensitivities with a model toxicant.
… as most molecular AOP interactions occur at the protein level and dictate biological structure-function relationships. Here, I first identified diverse procedures, practices, and reporting gaps present in the fish toxicology proteomics literature and recommended opportunities for development of …
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Effect of Naturally Occurring DNA Modifications on DNA Structure and Packaging
… These effects may provide clues to the differential protein recruitment observed in previous research. In vitro studies on nucleosome occupancy and stability revealed that 5-formylcytosine (fC) modifications have positive effects on nucleosome formation and stability compared to the …
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The Study of Wnt Signaling Effector POP-1/TCF in C. Elegans Early Embryos
… POP-1 nuclear asymmetry requires a 14-3-3 protein PAR-5 and at least three POP-1 potential phosphorylation sites for the MAPK LIT-1. LIT-1 activity is required for both POP-1/PAR-5 interaction and phosphorylation of at least two of the three potential LIT-1 sites in vivo. Nuclear export is …
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Application and integration of bioinformatic strategies towards central and peripheral proteomic profiling for diagnosis and drug discovery in schizophrenia
… approach. Models were trained based on either differential protein (chapter 3) or peptide (chapter 4) expression levels between schizophrenia patients and controls, as measured through multiplex immunoassay or targeted mass spectrometry technologies. In chapter 4, an SVM model based on 21 …
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Comprehensive definition of Ser/Thr/Tyr phosphorylation in mycobacteria: towards understanding reprogramming of normal macrophage function by pathogenic mycobacteria
… it employs to adapt and survive. We investigated protein regulation by the process of phosphorylation, through sensory kinases, which add a phosphate group to a protein of interest, thereby regulating its function. First, we interrogated the phosphoproteomic landscape between M. bovis BCG and M. …
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Semi-Synthesis of the Transcription Factor SMAD2 Containing Caging Groups and Phosphoaminoacid Analogues
<p>Post-translational modification (PTM) of a protein refers to any chemical change that occurs to the protein after its ribosomal synthesis. The seemingly endless number of PTMs can endow proteins with new functionalities that are not present in the unmodified proteins. In order to study the …
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Mechanical manipulation and visualization of single biomolecules using fluorescence spectroscopy
… the conformational dynamics of nucleic acids and proteins under physiological levels of tension is integral to interpreting their structure and function relationship in vivo. To address this, we employed an integrated force-FRET (Fluorescence resonance energy transfer) assay to interrogate single …
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