Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 42 for “"differential display"”.
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Systematische Analyse der Genexpressionsmuster in epithelialen Ovarialkarzinomen durch Differential Display
… gelang es, mit dem Verfahren des Differential Displays, unterschiedlich exprimierte Gene zu identifizieren, die an der Entstehung von Ovarialtumoren beteiligt sein könnten. Die gefundenen Unterschiede wurden durch quantitative PCR mit genspezifischen Primern an mehreren …
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Identifizierung differentiell exprimierter Gene nach zentraler und peripherer Nervenläsion mittels differential display PCR(ddPCR)
… process of regeneration in the nervous system, differential display polymerase chain reaction has been used to identify differntially expressed genes following axotomy of peripheral and central nerve fibers. For this purpose, axotomy induced changes of regenerating facial nucleus neurons and …
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Analyse der Annexin-1-Expression und Identifikation neuer Glukokortikoid-stimulierbarer Gene durch Differential-Display-RT-PCR-Methodik in humanen Makrophagenkulturen
… in monocytes/macrophages, using the differential-display-RT-PCR-method. The results show that neither on the gene level nor on the protein level the production of Annexin 1 in human monocytes and monocytic cultures is regulated by glucocorticoids. These results call into question its …
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Identifizierung und Charakterisierung entwicklungsabhängig hochregulierter Gene im Innenohr der Ratte
… der Ratte hochreguliert waren. In einem 'Differential Display' Experiment wurden 174 verschiedene Gene gefunden, die im Corti'schen Organ kurz nach dem Beginn der Hörfähigkeit höher exprimiert waren als am Tag der Geburt. Ca. 60% der identifizierten Transkripte codieren für Proteine …
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Gene expression in a microspore-derived cell suspension culture of Brassica Napus exhibiting enhanced oil production
… medium was increased from 2 to 14 % (w/v). mRNA differential display by polymerase chain reaction was used to examine gene expression in cells grown at different sucrose concentrations in order to identify mRNAs which could be associated with oil formation. The anchored primer, T12AA, was used to …
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Dissecting the jasmonate singalling pathway in Arabidopsis thaliana
… PYK10, was previously isolated using the differential display technique in an attempt to isolate methyl jasmonate-induced genes in Arabidopsis thaliana. β-glucosidases catalyse the breakdown of glucosinolates into glucose and sulphate releasing toxic by-products which play a role in plant …
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Characterization of Gene Expression During Adenosine 3':5'-Cyclic Monophosphate Induced Neuroendocrine Differentiation in Human Prostatic Adenocarcinoma
… Elucidation of those genes that are differentially expressed between undifferentiated prostate cancer cells and prostate cancer cells that have been induced to differentiate may present new insights for the molecular mechanisms governing neuroendocrine differentiation, early detection …
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Identification of differentially expressed genes in breast cancer tissues and cells
… The purpose of this project was to utilize the differential display technique to identify genetic changes in normal versus malignant breast tissue. It was also used in a defined cell culture system having differential cellular characteristics, to identify genes that may be responsible for …
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Characterization of two genes, trehalose-6-phosphate synthase/phosphatase and nucleotide binding protein, shown to be differentially regulated in roots of Cypripedium parviflorum var. pubescens grown with a mycorrhizal fungus Thanatephorus pennatus
… pathogenic pathways to establish symbioses. A differential display technique was employed to analyze gene changes in orchid in response to a fungus. Samples of RNA from roots of Cypripedium parviflorum var. pubescens (CyPP) grown in the presence or absence of a mycorrhizal fungus; Thanatephorus …
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Defining the ethanol-stress response in Saccharomyces cerevisiae
… and adapt to this stress. This thesis used differential display and gene array technologies to determine, at a molecular genetics level, how yeast cells adapt to sub-lethal concentrations of ethanol. Such information is of fundamental importance to the development of yeast strains and …
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Identification of Undifferentiated Human Embryonic Stem Cell-Specific Genes
… ES cells and various cancer cells by digital differential display of expressed sequence tag databases. Then, we confirmed differential expression of these genes by RT-PCR in human embryonic stem cell, neural stem cells and normal tissues, and identified that 3 genes, KIAA1922, SELV, and …
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Gene expression underlying ferret hair growth initiation induced by melatonin and modified by steroids
… hair growth was studied using an optimised differential display technique using two sets of complementary skin samples covering all growth initiation stages. The differential display band patterns for approximately 8% of all skin transcripts suggested that more than a thousand genes are …
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DIFFERENTIAL GENE EXPRESSION DURING ISCHEMIA AND REPERFUSION IN AN EXTRACORPOREAL SMALL BOWEL PERFUSION MODEL IN SWINE
… zu verwenden. Des weiteren konnten wir mittels Differential Display eine Vielzahl von hoch- und herunterregulierten Genen identifizieren, darunter das Schweine-Leukozytenantigen SLA-DQ (MHC Klasse II), dessen mRNA-Expression unmittelbar nach Reperfusion hochreguliert wird. MHC Klasse II ist für …
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Überexpression von s-Adenosylhomocysteinhydrolase in Epstein-Barr-Virus-positiven Burkitt-Lymphomzellen
… change in the activity of the examined genes by differential display These results show a way to elucidate the activation of the latent persisting EBV and its signal paths. This could be a step in finding new therapies against EBV-positive tumors.
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HECAT5 regulates cell proliferation via interaction with M3R in cell type-specific manner
… cells, but not normal tissues by using Digital Differential Display (DDD). Overexpression of HECAT5 promotes self-renewal capacity and it enhances tumorigenicity in cell type-specific manner. HECAT5 has effects on those through the interaction with M3R (Muscarinic acetylcholine receptor 3), …
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Paveldimas miežio viengubų ir dvigubų homeozinių mutantų fenotipo nestabilumas ir galimos jo priežastys /
… the auxin function. Preliminary results of the differential display analysis showed that the mutant Hv-tw2 differs from WT in the expression of several cDNA fragments that are homologous to the genes participating in epigenetic regulation, stress responses and flower development and that may be …
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Inherited phenotypic instability of barley homeotic single and double mutants and its possible causes /
… the auxin function. Preliminary results of the differential display analysis showed that the mutant Hv-tw2 differs from WT in the expression of several cDNA fragments that are homologous to the genes participating in epigenetic regulation, stress responses and flower development and that may be …
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Identification of up-regulated genes of the hyphomycete, Beauveria bassiana, during infection of Leptinotarsa decemlineata
… using cDNA RDA (i.e. in comparison with the differential-display). Eleven genes were specifically expressed or over-expressed in the cuticle medium cultures of B. bassiana. These genes encode three putative hydrolases (choline sulfatase, serine peptidase, a/b-hydrolase enzyme), three probable …
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Molecular Analysis of Neural Structures During Song Learning in the Zebra Finch
… the ingrowth of axons from HVC into RA. I used differential display PCR to identify myelin basic protein as another structural molecule which is developmentally regulated in the song circuit. Using a combination of in situ hybridization and immunocytochemistry, I showed that the protein abruptly …
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The role of proteases in development of cancer in mice
… cathepsins in tumour bearing tissues. Using mRNA differential display techniques, a number of different differentially expressed genes were identified in the tumour bearing and normal tissues. Further cloning and sequencing of these genes need to be carried out in the future.
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