Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"diaphorase"”.
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New Methods of DNA Assembly, Gene Regulation with a Synthetic sRNA, and Cyanobacterium Phenotype Monitoring with Raman Spectroscopy
… mechanism was translated for expression of a diaphorase enzyme, which has relevance to synthetic biology and metabolic engineering in in vitro systems. Results were successful, showing a greater than 75% increase in diaphorase expression in a cell-free protein synthesis reaction. Next, Raman …
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Mechanistic studies on aziridinylbenzoquinones and CCNU [l-(2-chloroethyl)- 3-cyclohexyl-l-nitrosourea] in cultured tumour cells
… by the presence of the reducing enzyme DT-Diaphorase in thecells. However we have shown that Es5 has a much higher partition coefficient thanRH1 and yet following cleavage of the ester group produces a quinone whoseCytotoxicity profile is very similar to RH1. The possibility of using Es5 as …
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NADPH-d staining in the supraoptic and paraventricular nuclei increases in late pregnant and lactating rats and is influenced by ovarian steroids and central oxytocin levels
… has demonstrated that staining for NADPH-diaphorase (NADPH-d) a histochemical marker for nitric oxide synthase is increased in the supraoptic (SON) and paraventricular (PVN) nuclei in late pregnancy. This work was extended to investigate whether increases in staining were evident at other …
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Poly(α-hydroxyalkanoic acid): Polymerization methodology, materials, and applications
… a caged metabolic precursor responsive to DT-diaphorase in mammalian cells. The decaging process was finely tuned and trigger responsive cell surface labeling was achieved.
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Development of Building Blocks - Thermostable Enzymes for Synthetic Pathway Biotransformation (SyPaB)
… glucose-6-phosphate dehydrogenase (G6PDH) and diaphorase (DI) and their fusion proteins G6PDH-DI and DI-G6PDH were cloned from Geobacillus stearothermophilus, heterologouely expressed in E. coli and purified through its His-tag. The individual proteins G6PDH and DI have good thermostability and …
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Investigations into the physiological actions of nitric oxide in the nervous systems of the ring dove and the rat
… (SCG) of the rat. The histological NADPH-diaphorase (NDP) technique was used to show the distribution of the NO synthesising enzyme nitric oxide synthase (NOS) for the first time in the ring dove brain. Previously, the NDP distribution has been mapped in the brain of other species and …
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Characterisation of Nitric Oxide Synthase Activity in the Sea Anemone Aiptasia pallida and an Evaluation of Responses to Environmental Stress
… localisation of NOS activity by NADPH-diaphorase staining showed the enzyme to be present in the epidermal cells and at the extremities of the mesoglea. The hypothesis that NOS has potential as an innovative biochemical effect biomarker in cnidarian species was investigated with a …
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Development of an Injectable Ablative Therapy for Resource-Limited Settings: Applications in Tumor Ablation
… sectioning, and staining it with NADH-diaphorase. There was an approximate one-to-one equivalence of the ethanol distribution volume with the necrotic volume for 12% ethyl cellulose-ethanol. This validates the concentration-based thresholding strategy utilized to determine the ethanol …