Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"diagnostic assay"”.
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Diagnostic assay and vaccine vector development for African Swine Fever Virus
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2024-12-01
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DEVELOPMENT OF A RAPID DIAGNOSTIC ASSAY FOR THE COLORIMETRIC DETECTION OF LEGIONELLA BACTERIA USING LOOP MEDIATED ISOTHERMAL AMPLIFICATION
… on agar plates. There are few rapid assays available that can identify multiple <em>Legionella </em>species. This project aims to develop a Loop Mediated Isothermal Amplification (LAMP) assay that can detect multiple species of <em>Legionella </em>in less than one hour. LAMP utilizes …
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Development and preliminary evaluation of a diagnostic assay for neurosyphilis using a reverse transscription-polymerase chain reaction approach
… (CSF) is non-specific. A treponemal serological assay, using CSF, the CSF-fluorescent treponemal antibody-absorption (FTA-ABS) is very sensitive but has a low specificity for neurosyphilis. On the other hand, the CSF-Venereal Disease Research Laboratory (VDRL) has high specificity for …
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Developing a diagnostic assay for Pneumocystis jirovecii pneumonia and investigating the interaction of Pneumocystis murina and respiratory syncytial virus during co-infection in mice
… To reduce the burden of disease, a reliable diagnostic test is necessary. Additionally, co-infections are common, especially in immunocompromised individuals, and are often linked to increased severity. In the Drakenstein Child Health Study, respiratory syncytial virus (RSV) was the most …
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DEVELOPMENT OF A RAPID DIAGNOSTIC ASSAY FOR THE COLORIMETRIC DETECTION OF BACTERIAL AND VIRAL PATHOGENS USING LOOP-MEDIATED ISOTHERMAL AMPLIFICATION COUPLED WITH A PEPTIDE NUCLEIC ACID AND GOLD NANOPARTICLE REPORTER SYSTEM
… and prevention of infectious disease. While diagnostic methods continue to break barriers, the need for accurate, sensitive, and rapid methods persists. Current methods for pathogen detection, including culture, immunochemical, or molecular-based techniques, are subject to significant …
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Molecular characterization of the major envelope protein of porcine reproductive and respiratory syndrome virus (PRRSV) and evaluation of its use for a diagnostic assay, vaccine development, and the examination of quasispecies evolution
… of PRRSV through genetic characterization, assay development, and quasispecies evaluation using the PRRSV ORF5 gene while also attempting to develop an improved PRRS vaccine. To explore the possible mechanism for the emergence of acute PRRS, the open reading frame 5 (ORF5) gene encoding the …
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Soybean Response to Plant Growth Regulator Herbicides: Field Evaluation and Expression Analysis of Candidate Auxin -Responsive Genes
A lack of diagnostic tools specific to PGR herbicides often result in difficulty and confusion when responding to reported cases of soybean symptoms resembling PGR herbicide injury. PGR herbicides overstimulate the auxin hormonal pathway and induce gene expression. Identifying genes specifically …
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Classification Tree Models for Predicting Cancer Status
… used as the laboratory method to develop the diagnostic assay for ovarian, breast, endometrial, and lung cancer. To evaluate the accuracy of early stage detection, logistic regression (with forward selection) and classification tree models were applied as statistical methods. Furthermore, …
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Design and fabrication of a microfluidies gradient generator system for high-throughput molecular interaction studies
… environments that can be used as a diagnostic assay and microreactor is important. Here, a simple technique was developed to create a robust microfluidics system capable of generating precise gradients of biochemical properties within its channels. Through this approach, it is …
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Applications of Engineered Proteins in Redox Biology and Biomarker Detection Assay Development
… very versatile tools that have been applied in assay development for various purposes. They have been made into genetically encoded biosensors/probes or affinity agents for biomarker detection. This thesis explored a few topics using assays developed with engineered proteins. The genetically …
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ANALYSIS OF ANIONS AND PROTEINS RELATED TO OXALATE METABOLISM IN HUMAN ERYTHROCYTES
… assessed a more costeffective and less-invasive diagnostic assay called Dried Blood Spot Filter Paper Assay the detection of Primary Hyperoxaluria Type 2 (PH2), a disease characterized by the dysfunction of GR/HPR. GR/HPR activity was detected in erythrocytes at levels similar other blood …
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Silaffin Tagged Polymerase for Nucleic Acid Amplification
There is an ever-growing demand for diagnostic relevant biological reagents. However, the high cost of these biomaterials limits their accessibility to burdened low- and middle-income countries. This thesis explores a synthetic biology approach to deliver a recombinant Geobacillus …
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Biochemistry: Production of recombinant Rh blood group antigens for detection of alloimmunisation
… utilised in this study may lead to a new diagnostic assay whereby the expression of human RhD external loops in E. coli may be capable of detecting speci c anti-Rh antibodies in patient serum.
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Mycobacterium tuberculosis strains in New Zealand: phylogeny and structural biology
… SNP analyses also guided the optimisation of a diagnostic assay for rapid Rangipo strain classification at low cost and with high discriminatory power. Phylogenetic analyses revealed the Rangipo and Otara clusters belong to a larger M.tuberculosis clade of French/European origin that is also …
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Assessment of taxol activity: Bioavailability in human physiological fluids and QSAR of taxol analogues based on a neural network design
… a competitive, enzyme-linked immunosorbent assay (ELISA) to quantify bioavailability of taxol in human fluids: plasma, plasma ultrafiltrate and salivary fluids. An optimization procedure decreased the detection limit to pg/ml. The working range encompasses five orders in magnitude, i.e. from …
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Paper-based Molecular Technologies for Faster, More Accessible Infectious Disease Diagnostics
… the cost and infrastructure needs of current diagnostic methods. Technologies that utilize low-cost materials and easy-to-use methods could help address this issue. Diagnostic tests made from cellulose paper that use colorimetric readouts—where a visible color appears if disease biomarkers are …
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Development of an efficient test for autoimmune disease using gold nanoparticles
… as specific biomarkers for developing reliable diagnostic tests. Over the years, different approaches like the use of mucosal biopsy, genetic typing of the disease associated gene, gliadin induced cytokines, antibodies and auto-antibodies have been used to develop diagnostic tests for coeliac …
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Defining the genetic and molecular pathways underlying motor neuron degenerative diseases
… there are currently no universal biomarker assays or disease-modifying therapies available for the majority of affected individuals, primarily due to a lack of understanding of the pathomolecular causes of disease. The identification of disease-associated genes provides a direct link between …
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From Sample to Answer: Innovations in sample processing and CRISPR-based diagnostics for enhanced clinical translation and field deployment
… and control strategies. Accurate and timely diagnostics serve as the base for effective disease management, enabling the rapid identification of disease outbreaks, guiding treatment decisions, and informing public health interventions. However, the global diagnostic testing capacity is …
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3D printing in Clinical Diagnostics: A Versatile Tool to Develop Testing Devices for the Diagnosis of Infectious Diseases
… use of rapid manufacturing techniques to develop diagnostic assay kits for various diseases; predominantly in the development of point-of-care (POC) devices through the combination of microfluidic and microelectromechanical systems (MEMS). 3DP techniques can be utilized to develop various fluidic …
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