Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"diacylglycerol (DAG)"”.
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Cloning and charaterisation of the Thyrotrophin-releasing hormone receptor and Gonadotrophin-relasing hormone receptor from chicken pituitary gland
… in turn triggers the secondary messenger [1,2- diacylglycerol (DAG) and inositoltrisphosphate (IP3)] signalling cascade. The signalling generates the physiological effects of the hormones. The TRH-R and GnRH-R are members of G-protein coupled receptor (GPCR) family. The objective of this thesis …
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Understanding the biochemical basis of temperature induced lipid pathway adjustments in plants
… Evidence of differential trafficking of diacylglycerol (DAG) moieties from ER to chloroplast was uncovered in three plant species as another layer of metabolic adaptation under different temperatures. Taken together, this study has established a biochemical basis that highlights the …
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Biochemical and physiological studies of Arabidopsis thaliana Diacylglycerol Kinase 7 (AtDGK7)
A family of diacylglycerol kinases (DGK) phosphorylates the substrate diacylglycerol (DAG) to generate phosphatidic acid (PA) . Both molecules, DAG and PA, are involved in signal transduction pathways. In the model plant Arabidopsis thaliana, seven candidate genes (named AtDGK1 to AtDGK7) code for …
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Interplay of DGAT1, PDAT1 and DGAT2 Enzymes in Plant Triacylglycerol Assembly
<p>Acyl-CoA:diacylglycerol acyltransferase (DGAT) catalyzes the transesterification of fatty acid from acyl-CoA to diacylglycerol (DAG) forming triacylglycerol (TAG, a.k.a oils and fats). Most plants have at least two unrelated <em>DGAT</em> genes, <em>DGAT1</em> and <em>DGAT2.</em> Plants …
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Regulation of RhoA Activation and Actin Reorganization by Diacylglycerol Kinase
… RhoGDI. For example, our laboratory showed that diacylglycerol kinase zeta (DGKz), which converts diacylglycerol (DAG) to phosphatidic acid (PA), activates PAK1-mediated RhoGDI phosphorylation on Ser-101/174, causing selective Rac1 release and activation. Phosphorylation of RhoGDI on Ser-34 by …
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Intrinsic Mechanisms that Regulate T Cell Homeostasis and Function
… </p><p> In the second model, I utilized diacylglycerol kinase (DGK) deficient mice in our examination of the mechanisms by which the TCR affects T cell biology. To date, ten DGK isoforms have been identified in mammals, with DGKα and DGKζ being expressed in T cells. Immediately following …
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The Immunomodulatory Functions Of Diacylglycerol Kinase Zeta On Type 2 Immune Responses
… vivo in a polyclonal setting. The lipid molecule diacylglycerol (DAG) is the main driver of TCR-mediated ERK activation. Here, we demonstrate that T cells lacking DAG kinase-ζ (DGKζ), a negative regulator of DAG, display impaired Th2 differentiation in vitro. Accordingly, mice lacking DGKζ …
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Munc18 function in large dense-core vesicle exocytosis
… potentiation of catecholamine release in the diacylglycerol (DAG) route. The DAG pathway remains an important but poorly understood regulator of transmitter/hormon release, which probably has multiple branches targeting multiple molecules and exocytic steps. Taken together, our findings …
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The Regulation of Lipid Metabolism and Mitochondrial Quality Control in Health and Disease
… shortly after mitochondrial damage while diacylglycerol (DAG) appears several hours later. This lipid accumulation is dependent upon Parkin's translocation and E3 ligase activity. Additionally, we found that lipin-1, a PA phosphatase, and endophilin B1 (EndoB1) are critical for DAG …
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Role of diacylglycerol in the cellular response to lysophosphatidylcholine analogues
Diacylglycerol (DAG) is a key signaling lipid and intermediate in lipid metabolism. Our knowledge of DAG distribution in cell membranes is limited. Using the C1 domain from mammalian PKCδ and PKD, we monitored the localization of yeast cytosolic-facing pools of DAG for the very first time and …
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Inhibition of AMPK via phosphorylation at Ser485/491: multiple mechanisms of regulation
… is regulated. We investigated whether insulin or diacylglycerol (DAG) signaling pathways may be involved, since both are increased in at least one of the HG models. Akt and Protein Kinase (PK)D1 phosphorylated AMPK at Ser485/491 and diminished its activity in C2C12 myotubes, downstream of insulin …
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Determination of the roles of the Trypanosoma brucei lipin homologue TbLpn
… examined. Lipins help catalyze the formation of diacylglycerol (DAG), which is used to form triacylglycerol (TAG). TAG is then used to form phosphatidylethanolamine (PE) and phosphatidylcholine (PC), which are major lipids that constitute the membrane of T.b. In order to better understand how …
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Dual Regulation of Phospholipase C-beta by G betagamma
… messengers, inositol trisphosphate (IP3) and diacylglycerol (DAG). PLC-b isoforms are also GTPase activating proteins (GAPs) for Gaq, and Gbg subunits inhibit the GAP activity of PLC-b. Coordinated regulation of these activities is essential for sustained signaling at steady state. Regulation …
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From Cell Shape to Body Shape: Epithelial Morphogenesis in Drosophila melanogaster
… Signaling (RGS) domain and a cysteine-rich diacylglycerol (DAG)-binding domain in the central region. Previously, DRhoGEF2 has been shown to play an important role in the constriction of Actin filaments during pole cell formation, blastoderm cellularization, and invagination of the germ …
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Bioactive Lipids in Nociception
… of phosphatidylinositol bisphosphate into diacylglycerol (DAG) and inositoltrisphosphate, diacylglycerol may be further metabolized to monoacyl-glycerols, such as 2-arachidonoylglycerol (2-AG) and 2-oleoylglycerol by the action of DAG lipase. 2-Arachidonoylglycerol and 1-AG activate both …
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PKC-δ and Insulin Resistance in Non-Alcoholic Fatty Liver Disease: Effect of Vitamin D
… Protein kinase C (nPKC), is solely activated by diacylglycerol (DAG). Diacylglycerol has been found to be increased in the liver during NAFLD. Several studies have demonstrated a link between vitamin D deficiency, high fructose diet, and NAFLD in human and rodents. The specific aim of this study …
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An Investigation into the Function of CDS2 in Phosphoinositide Synthesis in Murine Macrophages
… messengers inositol (1,4,5)-trisphosphate and diacylglycerol (DAG) from phosphatidylinositol (4,5)-bisphosphate, resulting in a net loss in the cellular PIPn pool which is replenished by the activation of phosphatidylinositol (PI) synthesis. A critical intermediate for PI synthesis is cytidine …
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Localisation and function of phosphoinositide-specific phospholipase C in Sacchromyces cerevisiae
… (1,4,5) trisphosphate [Ins(1,4,5)P\(_3\)] and diacylglycerol (DAG). Ins(1,4,5)P\(_3\) is an important second messenger in animal cells. It releases calcium from intracellular stores by opening Ins(1,4,5)P\(_3\) receptors (InsP\(_3\)Rs) and is formed in response to activation of cell surface …
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The regulation of yeast homotypic vacuole fusion by phosphatidic acid and diacylglycerol
… includes ergosterol, phosphatidic acid (PA), diacylglycerol (DAG), and phosphoinositides. However, the interactions and effects each of these lipids has throughout the stages of membrane fusion has not been elucidated. This project aimed to identify specific regulatory roles for the lipids PA …
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Novel lipid regulation of vacuolar fusion in saccharomyces cerevisiae
… interest is the lipid phosphatidic acid (PA) and diacylglycerol (DAG) which are interconverted by the PA phosphatase Pah1 and the DAG kinase Dgk1. Previous work showed DAG acts as a positive regulator of vacuolar fusion and is proposed to reduce the force requirements of vacuolar fusion. I …
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