Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 71 for “"diacylglycerol"”.
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Diacylglycerol lipaseα-dependent endocannabinoid signaling in neural stem cells
The diacylglycerol lipases (DAGL α and β) synthesise 2-AG, the major endocannabinoid (eCB) in the developing and adult brain. 2-AG acts most prominently on the CB1 and CB2 receptors and growing evidence points to a role of eCB signaling in adult neurogenesis as well as a wide range of different …
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Investigating mechanisms of triacylglycerol synthesis induced by nitrogen depletion in the diatom Phaeodactylum tricornutum and characterising its diacylglycerol acyltransferases and phospholipid: diacylglycerol acyl transferase
… chloroplast lipids, particularly monogalactosyl diacylglycerol (MGDG), during disassembly of thylakoid membranes under N-depletion. Identifying MGDG derived TAG accumulation may yield enzymes that target EPA to TAG and thereby enable use of developed extraction processes. Some archaeplastida also …
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Regulation of RhoA Activation and Actin Reorganization by Diacylglycerol Kinase
… RhoGDI. For example, our laboratory showed that diacylglycerol kinase zeta (DGKz), which converts diacylglycerol (DAG) to phosphatidic acid (PA), activates PAK1-mediated RhoGDI phosphorylation on Ser-101/174, causing selective Rac1 release and activation. Phosphorylation of RhoGDI on Ser-34 by …
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Role of diacylglycerol in the cellular response to lysophosphatidylcholine analogues
Diacylglycerol (DAG) is a key signaling lipid and intermediate in lipid metabolism. Our knowledge of DAG distribution in cell membranes is limited. Using the C1 domain from mammalian PKCδ and PKD, we monitored the localization of yeast cytosolic-facing pools of DAG for the very first time and …
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Regulation of Diacylglycerol Acyltransferase-2 and Triacylglycerol Synthesis by Protein Ubiquitination
… Triacylglycerol synthesis is catalyzed by diacylglycerol acyltransferase (DGAT) enzymes, DGAT1 and DGAT2. Although recent studies have shed light on the metabolic functions of these enzymes, little is known about their regulation. We have found that DGAT2 is a short-lived protein and is …
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Biochemical and physiological studies of Arabidopsis thaliana Diacylglycerol Kinase 7 (AtDGK7)
A family of diacylglycerol kinases (DGK) phosphorylates the substrate diacylglycerol (DAG) to generate phosphatidic acid (PA) . Both molecules, DAG and PA, are involved in signal transduction pathways. In the model plant Arabidopsis thaliana, seven candidate genes (named AtDGK1 to AtDGK7) code for …
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The Immunomodulatory Functions Of Diacylglycerol Kinase Zeta On Type 2 Immune Responses
… vivo in a polyclonal setting. The lipid molecule diacylglycerol (DAG) is the main driver of TCR-mediated ERK activation. Here, we demonstrate that T cells lacking DAG kinase-ζ (DGKζ), a negative regulator of DAG, display impaired Th2 differentiation in vitro. Accordingly, mice lacking DGKζ …
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The regulation of yeast homotypic vacuole fusion by phosphatidic acid and diacylglycerol
… includes ergosterol, phosphatidic acid (PA), diacylglycerol (DAG), and phosphoinositides. However, the interactions and effects each of these lipids has throughout the stages of membrane fusion has not been elucidated. This project aimed to identify specific regulatory roles for the lipids PA …
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Regulation of PKCalpha and the role of PKC in neuroblastoma cell migration
… in Ca2+ and PKCalpha does not respond to diacylglycerol. In this work we have found that autophosphorylation of two sites in the V5 domain of PKCalpha keeps the protein insensitive to diacylglycerol, presumably due to masking of the diacylglycerol-binding C1a domain. We have identified …
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Elucidating the Role of Yeast Lipin (PAH1) in Lipid Droplet Biogenesis
… is an enzyme that converts phosphatidic acid to diacylglycerol, and its absence or elimination of its catalytic activity results in the accumulation of neutral lipids within membranes of the endoplasmic reticulum. Furthermore, lipid droplet formation is facilitated by diacylglycerol through a …
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Biosynthesis and Characterization of Early Glycosylphosphatidylinositol Anchor Intermediates in Saccharomyces Cerevisiae
… very long chain fatty acids, most likely on its diacylglycerol moiety. This has significant implications for GPI anchor biosynthesis in yeast and suggests that GPI anchors are either assembled on a specialized PI or that the diacylglycerol moiety on early GPI precursor molecules are rapidly …
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Formation of membrane domains during the activation of protein kinase C
… kinase C. However, only Ca$\sp{2+}$ could induce diacylglycerol to partition into the phosphatidylserine domains. In the complete protein kinase C assay mixture, two separate triple-labeling experiments demonstrated the colocalization of phosphatidylserine, protein kinase C, diacylglycerol, and …
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Therapeutic targeting of DGKA-mediated macropinocytosis in lymphangioleiomyomatosis
… drug library. We identified that ritanserin, a diacylglycerol kinase alpha (DGKA) inhibitor, synergizes with Chloroquine (CQ) to selectively inhibit proliferation of TSC2-deficient mouse embryonic fibroblasts (MEFs) compared to TSC2+/+ MEFs. OBJECTIVE: We hypothesized that TSC2-deficient cells …
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An Investigation into the Function of CDS2 in Phosphoinositide Synthesis in Murine Macrophages
… messengers inositol (1,4,5)-trisphosphate and diacylglycerol (DAG) from phosphatidylinositol (4,5)-bisphosphate, resulting in a net loss in the cellular PIPn pool which is replenished by the activation of phosphatidylinositol (PI) synthesis. A critical intermediate for PI synthesis is cytidine …
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Development of Mass Spectrometric Methods for the Analysis of Components and Complex Interactions in Biological Systems
… ions of a lithiated mono-acylglycerol, a 1, 2-diacylglycerol, and a 1, 3-diacylglycerol, to predict the covalent bond dissociation energies involved in fragmentation pathways. The model's calculated apparent reaction enthalpies are used in conjunction with the energy-resolved mass spectrometry …
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Bioactive Lipids in Nociception
… of phosphatidylinositol bisphosphate into diacylglycerol (DAG) and inositoltrisphosphate, diacylglycerol may be further metabolized to monoacyl-glycerols, such as 2-arachidonoylglycerol (2-AG) and 2-oleoylglycerol by the action of DAG lipase. 2-Arachidonoylglycerol and 1-AG activate both …
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PKC-δ and Insulin Resistance in Non-Alcoholic Fatty Liver Disease: Effect of Vitamin D
… Protein kinase C (nPKC), is solely activated by diacylglycerol (DAG). Diacylglycerol has been found to be increased in the liver during NAFLD. Several studies have demonstrated a link between vitamin D deficiency, high fructose diet, and NAFLD in human and rodents. The specific aim of this study …
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