Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"developmental competence"”.
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Maturation and Culture Media Effects on In Vitro Bovine Embryo Developmental Competence
… maturation and culture media to improve the developmental competence of <em>in vitro </em>bovine embryos. This thesis is comprised of three experiments and one proof of concept study. Each experiment followed the same general layout: oocyte aspiration from Jersey or Holstein ovaries, oocyte …
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Effects of Polydatin on In Vitro Bovine Embryo Developmental Competence, Metabolism, and Cryopreservation
… vitro</em> produced embryos suffer from poor developmental competence and altered metabolism which hinders their cryotolerance. Overall, the goals of this thesis were to improve oocyte and embryo culture with the antioxidant polydatin and to optimize slow freezing procedures. This thesis was …
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Transcriptional profiles of cumulus-oocyte complexes related to developmental competence in bovine oocytes
During folliculogenesis, oocytes and cumulus cells undergo many morphological and physiological changes. Transcriptome data were produced from single oocytes and corresponding cumulus cells (CC) to infer the differences in the transcript abundance from fully grown versus growing phase oocytes and …
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Role of oocyte-secreted factors in prevention of cumulus cell apoptosis and enhancement of oocyte developmental competence.
… by OSFs may in turn contribute to oocyte developmental competence. The aim of this thesis was to examine whether cumulus cells exhibit a low incidence of apoptosis due to their close association with oocytes and their exposure to OSFs, and to investigate if OSFs have a direct influence on …
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A Genomic Perspective on Early Embryo Development in Cattle
… undergo pre-implantation development and become developmentally competent. Several research groups have been investigating highly dynamic gene expression regulation during early embryo development and its relationship to embryonic phenotypes. While it has been fundamental to improve our …
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MATERNAL DETERMINANTS OF OOCYTE AND EMBRYO QUALITY
… the quality of oocyte, the acquisition of oocyte developmental competence seems to mainly occur during the final stage of oocyte maturation. The correct temporal regulation of series of molecular events and the proper exchange of signals with surrounding follicular environment during this critical …
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Úloha SIRT1 během zrání oocytů v podmínkách in vitro
… progress. Keywords: oocyte, SIRT1, histone, developmental competence, tubuline, epigenetics
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Genomic studies of oocyte physiology reveal mechanisms critical to oocyte competence
… and methods to characterize and improve the developmental competence of in vitro matured porcine oocytes. Medium environment impacts the metabolism and physiology of oocytes. Using chemically defined medium, we demonstrated the detrimental effects of ammonium on porcine oocyte maturation and …
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Dynamic study of the piezo-driven pipettes in intracytoplasmic sperm injection
… both related to personnel safety and to the developmental competence of the manipulated oocytes. Therefore, this research is developed to analyze the effects of mercury on the dynamic characteristics of the piezo-driven pipette, and to achieve the improved method to replace mercury. The …
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Maternal childhood risk status as a predictor of emotional availability and physical contact in mother-child interactions : an intergenerational study
… risk status, maternal scaffolding and child developmental competence was also examined. Participants were recruited from the subjects making up the Concordia Risk Project, which commenced in 1977 when children in Grades 1, 4, or 7, from low SES, inner-city neighbourhoods, were classified …
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Profiles of Risk, Protection, and Socioemotional Adjustment Among Children Exposed to Intimate Partner Violence: A Person-Centered Approach
… childhood is associated with a host of negative developmental consequences. Research in this area has also identified several interconnected risk and protective factors that contribute to heterogeneity in adjustment among children exposed to IPV. To date, few studies have applied person-centered …
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Towards elucidating the function of the oocyte Subcortical Maternal Complex: A multi-omic analysis of an Nlrp5-null murine model
… NALP5 and, by extension, the SCMC, in oocyte developmental competence. This study focuses primarily on elucidating the role of NALP5 in the context of DNA methylation patterning in the oocyte. This was done by establishing a null mouse line for *Nlrp5*, the gene encoding NALP5. A phenotypic …
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Karyopherin alpha subtypes and porcine early stage embryo development
… The objective of this study was to determine the developmental requirements of karyopherin α subtypes (KPNAs) during cleavage development in porcine embryos. The purpose of this dissertation was to test the hypothesis that the karyopherin α/β heterodimer-mediated nuclear trafficking pathway serves …
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MICRORNA EXPRESSION WITHIN PERIOVULATORY MURAL GRANULOSA CELLS
… is dependent on the regulation of oocyte developmental competence, ovulation and corpus luteum function as each makes an important contribution to proper ovarian function. During the periovulatory period, ovulation is initiated as a surge of LH induces granulosa cells to rapidly transform …
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Multi-omics characterisation of mouse gastrulation and organogenesis at single-cell resolution
… cell lineage specification and establishment of developmental competence. In sum, my PhD project provides a detailed study of the role of the spatial environment on transcriptional regulation (Aim 1) and the causal relationship between DNA methylation and cell fate in early mammalian …
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Exploring methods to understand bovine embryo competency in vitro
The development of a preimplantation embryo is a stepwise process consisting of morphological, biochemical, and genomic changes. Much remains unknown about the attainment of embryo competency to develop and establish pregnancy. To investigate this, we compared methods of selection at the oocyte or …
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Public sector senior management competencies required for the South African democratic, developmental state
Adopting the philosophy of a developmental state proved to have a distinct attractiveness to nations around the world as it offers rapid economic growth and global recognition. In recognising that such an offering comes at an extraordinary price, this study introduces and then explores the nature …
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Management and nutrition of the replacement gilt.
… restriction on ovarian development and oocyte developmental competence in 161- and 175- day old, pre-pubertal gilts. Both chronic and acute periods of moderate feed restriction reduced the number of medium follicles present on the ovaries of 161- and 175-day old gilts, as well as the proportion …
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DISSECTING THE ROLE OF REACTIVE OXYGEN SPECIES IN EARLY MAMMALIAN EMBRYOGENESIS
… Therefore, the observed reduction in embryonic developmental competence is likely not due to mt-H2O2 suppression but rather their increased production due to the paradoxical effect of high antioxidant concentrations. In conclusion, we established a method to quantitatively measure mitochondrial …
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Signalling and transcriptional regulation of human primordial germ cell specification
… if the cells are first cultured in PGC competence-promoting media. To address how the PGC-competent state might be gained, I used a new computational tool – Branch-Recombinant Gaussian Processes (B-RGPs) – to identify potentially relevant pathways based on single-cell RNA sequencing of …