Global ETD Search
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Showing 1 to 15 of 15 for “"deubiquitinating enzymes"”.
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STRUCTURAL AND BIOCHEMICAL CHARACTERIZATION OF DEUBIQUITINATING ENZYMES, SST2 AND SSEL
Associated molecule with SH3 domain of molecule (STAM), or AMSH, is a
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Deubiquitinating Enzymes Promote Cancer Progression and Metastasis Via Regulating Protein Stability
<p>Deubiquitinating enzymes (DUBs, also called deubiquitinases) are enzymes that remove monoubiquitin or polyubiquitin chains from target proteins. DUBs have critical roles in cell homeostasis and signal transduction, as they regulate protein degradation, subcellular localization, and …
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An investigation into the role of deubiquitinating enzymes in plant disease resistance
… the sequential activities of E1, E2 and E3 enzymes leading either to proteasomal degradation or other regulatory outcomes in the cell. It is now established that ubiquitination is a reversible process and that removal of ubiquitin from target proteins by deubiquitinating enzymes (also termed …
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The Evolution of the Deubiquitinating Enzyme Superfamily
… details the evolution of members of the deubiquitinating enzyme superfamily. The first manuscript delineates the chronology of USP4, USP15 and USP11 emergence and concludes that the presumed ancestor, USP11, is in fact a recent duplicate and that, at minimum, one copy of USP4 or USP15 is …
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Cellular Determinants Of AURKA Degradation By Small Molecule Proteolysis-Targeting Chimeras
… as substrate localisation and the activity of deubiquitinating enzymes (DUBs), influence PROTAC efficacy, using the oncogenic kinase Aurora kinase A (AURKA) as a model substrate. Many small molecule inhibitors of AURKA have failed during clinical development due to dose-limiting toxicities and …
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Detection and measurements of free ubiquitin in fixed cells and characterization of OTUB1 contribution to ubiquitin homeostasis
… concentration of free Ub, is by means of Deubiquitinating enzymes (DUBs), however specific details about the regulatory mechanism are, in large part, unknown. Most studies about DUBs have focused on enzymatic activity and regulation in vitro, with only few reports on the regulation of Ub …
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Chemical and biochemical studies of ubiquitin conjugation machinery
… an enzymatic cascade catalyzed by E1, E2, and E3 enzymes. Ub conjugation is important for fundamental cellular processes, including transcription, DNA repair, endocytosis, apoptosis, and signal transduction. Ub conjugation is reversible. Proteases termed deubiquitinating enzymes (DUBs) function to …
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The Role of K63-Linked Ubiquitination Cycles In Akt Kinase Activation
… of protein ubiquitination can be reversed by deubiquitinating enzymes (DUBs), which play a critical role to terminate signaling activation induced by ubiquitination. To further investigate how ubiquitination cycles regulate Akt activation, we have identified that CYLD as a DUB for Akt, and …
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Analysis and confirmation of the results of a yeast two-hybrid screen carried out to identify proteins that interact with drosophila XRCC2
… protein. FAF belongs to a large family of deubiquitinating enzymes that cleave ubiquitin-protein bonds and play diverse roles in the ubiquitin pathway. One of the implications of such discoveries could be to compare and contrast DmXRCC2 and human XRCC2 in terms of their interactions and …
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The Role of the Ubiquitin-Proteasome Pathway During Xylem Differentiation in <I>Zinnia elegans</I> Mesophyll Cells and <I>Arabidopsis thaliana</I>
… Ub pathway components, including Ub-conjugating enzymes (<I>UBCs</I>), deubiquitinating enzymes (DUBs), and an Alpha (<I>PAF1</I>) and Beta (<I>PAF1</I>) subunit of the proteasome. All targeted <I>UBC</I> families, candidate <I>UBCs</I> and DUBs, and proteasome subunits are expressed in secondary …
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Structural and biophysical analysis of the proteasomal deubiquitinase, UCH37
… carboxyl-terminal hydrolase 37, or UCH37, is a deubiquitinating enzyme associated with the 26S proteasome, the primary protein degradation machinery in eukaryotic cells. UCH37 is responsible for the disassembly of polymeric ubiquitin chains, or polyubiquitin, which have been ligated onto …
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Species Preference of Viral Deubiquitinating Proteases Toward ISG15 Through Structural and Enzymatic Characterization
… the Ovarian Tumor domain (OTU) superfamily of deubiquitinating enzymes (DUBs) are expressed by a range of RNA viruses. Viral OTUs (vOTUs) are found in nairoviruses such as Crimean Congo Hemorrhagic Fever virus (CCHFV), Nairobi sheep disease virus (NSDV), the Erve virus (ERVEV), and the Dugbe …
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Structural and Functional Characterization of the Endosome-associated Deubiquitinating Enzyme AMSH
… is the removal of ubiquitin, catalyzed by deubiquitinating enzymes (DUBs). The human ESCRT machinery recruits two DUBs: AMSH (associated molecule with a Src homology 3 (SH3) domain of signal transducing adaptor molecule (STAM) or simply, STAM-binding protein (STAMBP)), and UBPY/USP8 …
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Inhibition of Deubiquitinase Activity and Ubiquitination of Jak2 Blocks Cytokine Signaling and Induces Tumor Cell Apoptosis
The Jak-stat pathway is critical for cellular proliferation and is commonly found to be deregulated in many solid tumors as well as hematological malignancies. Such findings have spurred the development of novel therapeutic agents that specifically inhibit Jak2 kinase, thereby suppressing tumor …
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Post-translational modifications of c-FLIP: opportunities for therapeutic intervention
… of ITCH did not appear to be important. No deubiquitinating enzymes (DUBs) have been associated with FLIP. To resolve this, we performed two screens: one to identify novel DUBs which may affect FLIP'S ubiquitination and a mediumthroughput luciferase-based screen to identify potential E3 …