Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"deubiquitinating enzyme"”.
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The Evolution of the Deubiquitinating Enzyme Superfamily
… details the evolution of members of the deubiquitinating enzyme superfamily. The first manuscript delineates the chronology of USP4, USP15 and USP11 emergence and concludes that the presumed ancestor, USP11, is in fact a recent duplicate and that, at minimum, one copy of USP4 or USP15 is …
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Structural and Functional Characterization of the Endosome-associated Deubiquitinating Enzyme AMSH
… is the removal of ubiquitin, catalyzed by deubiquitinating enzymes (DUBs). The human ESCRT machinery recruits two DUBs: AMSH (associated molecule with a Src homology 3 (SH3) domain of signal transducing adaptor molecule (STAM) or simply, STAM-binding protein (STAMBP)), and UBPY/USP8 …
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The role of the deubiquitinating enzyme CYLD and its substrate BCL-3 in solid tumors
… is not fully understood. CYLD is a deubiquitinating enzyme known to specifically target lysine 63 linked ubiquitin chains, which can negatively regulate the BCL-3, NF-κB and JNK signaling pathways, leading to a decrease of cell survival or proliferation. BCL-3 is an alternative IκB …
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Identifying Novel Interaction Between CDK8 Kinase Module and The Deubiquitinating Enzyme Ubiquitin Specific Protease 7 (USP7)
Ubiquitin Specific Protease 7 (USP7) is a deubiquitinating enzyme that regulates and interacts with various cellular substrates, playing regulatory roles in various pathways such as DNA damage repair and epigenetics. This thesis explores the role of USP7 in regulating components of gene expression …
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An investigation of the role of CreB deubiquitinating enzyme in the regulation of carbon metabolism in Aspergillus nidulans
… HulA, and deubiquitination, mediated by the deubiquitinating enzyme CreB, are involved in the regulatory pathway in A. nidulans. However, the molecular mechanisms are still unknown. Previously, partial loss-of-function alleles of creA and creB indicated genetic interaction, and this was …
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Tumor Suppressor function of the deubiquitinating enzyme BAP1 and its substrate gamma-tubulin In regulation of cell cycle and genome stability
… we found that the g-tubulin deubiquitination enzyme, BRCA1 Associated Protein 1 (BAP1), was downregulated in metastatic adenocarcinoma breast cell lines compared to non-cancerous human breast epithelial cells. Reduced expression of BAP1 in breast cancer cell lines was associated with mitotic …
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Identifying Novel Interaction Between the E3 Ubiquitin Ligase Ring Finger Protein 20 (RNF20) and the Deubiquitinating Enzyme Ubiquitin Specific Protease 7 (USP7)
… a ubiquitin-specific protease, is an essential deubiquitinating enzyme involved in a plethora of cellular processes such as epigenetic regulation, DNA damage response and oncogenic processes. In this study, RNF20, which is an E3 ligase, has been identified as a novel substrate for USP7. Through …
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Characterizing The Role of Uchl5 In Metastatic Melanoma
… as one of the top candidates. UCHL5 is a deubiquitinating enzyme and interacts with the 26S proteasome complex and the INO80 chromatin remodeling complex. While UCHL5 has been shown to be overexpressed in many cancers, it has not been well characterized in melanoma. We investigated the …
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Polyubiquitin chain editing by UCH37 promotes degradation of a subset of proteasome substrates
… chains. UCH37, a proteasome-associated deubiquitinating enzyme (DUB), has been shown to target the K48 linkage in branched polyubiquitin chains of diverse architectures. Additionally, this debranching activity has been shown to promote proteasomal degradation of branched chain-modified …
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The role of UCH-L1 in mouse progenitor spermatogonia
… fate decision is not completely understood. The deubiquitinating enzyme Ubiquitin C-terminal Hydrolase L1 (UCH-L1) is highly expressed in spermatogonia, but not in further differentiating germ cells of the testis. Previous studies have shown that UCH-L1 is important for normal spermatogenesis; …
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SORTASE AND CHEMICAL BIOLOGY AS TOOLS TO STUDY UBIQUITINATION
… the power of sortase, a bacterial transpeptidase enzyme crucial to cell wall synthesis, to develop new strategies for studying protein ubiquitination. As one new technology, we introduce point mutations to the ubiquitin sequence in combination with click chemistry and genetic code expansion to …
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Regulation of Wingless secretion, distribution and signaling
… Wls ubiquitiation, the E3 ligase Su(dx) and the deubiquitinating enzyme USP8. My work suggests that Wls ubiquitination is involved in the trafficking of Wls and the sorting of Wg following Wls endocytosis. Finally, I identified a novel component essential in the signal transduction of canonical …
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Cyclin B1 Mediates The Effect of Uchl1 In Promoting Cell Cycle Progression In Uterine Papillary Serous Carcinoma
… cohort of tumor samples. Due to its role as a deubiquitinating enzyme, we hypothesized that UCHL1 contributes to UPSC tumor progression by modulating the protein stability of target genes.</p> <p>To test this hypothesis, we first examined the functional role of UCHL1 in UPSC progression. …
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The Role of Arabidopsis thaliana P80 in Inositol Signaling
… the lab have also shown that P80 is a part of a deubiquitinating enzyme complex along with WDR20 and Ubiquitin-specific protease called UBP3. Our p80 mutants have a root deficient phenotype under low energy conditions which is normalized by addition of sucrose. p80 mutants show reduced growth and …
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Interrogating DNA replication stress and DNA damage responses via phenotypic and functional CRISPR/Cas9 screens
… on these screens, I provide evidence for the deubiquitinating enzyme USP37 regulating RPA loading and DNA repair during DNA replication.
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On aging and the role of Ubp3 in heterochromatic gene silencing and protein quality control
… Specific interest was directed towards the deubiquitinating enzyme Ubp3 which had already been shown to be tightly linked to regulation of transcription and proteome surveillance, both of which are essential in cells adaptation to stress. In this thesis, I show that cells lacking Ubp3 are …
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Structural and biophysical analysis of the proteasomal deubiquitinase, UCH37
… carboxyl-terminal hydrolase 37, or UCH37, is a deubiquitinating enzyme associated with the 26S proteasome, the primary protein degradation machinery in eukaryotic cells. UCH37 is responsible for the disassembly of polymeric ubiquitin chains, or polyubiquitin, which have been ligated onto …
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An investigation into the role of deubiquitinating enzymes in plant disease resistance
… the sequential activities of E1, E2 and E3 enzymes leading either to proteasomal degradation or other regulatory outcomes in the cell. It is now established that ubiquitination is a reversible process and that removal of ubiquitin from target proteins by deubiquitinating enzymes (also termed …
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Targeting mutant p53 in cSCCs
… p53 in cSCCs.<br/><br/>5. PR-619, a broad range deubiquitinating enzyme (DUB) inhibitor, reduces mutant p53 protein levels in a range of cSCC cell lines. This is rescued by the addition of bortezomib suggesting that DUBs can play a role in protecting mutant p53 from proteasomal degradation. …