Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 4 of 4 for “"detergent solubilization"”.
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Intracellular Trafficking of Influenza Hemagglutinin and Members of the Low Density Lipoprotein Receptor Family
… are operationally defined by their resistance to detergent solubilization at cold temperatures. By systematic mutagenesis of the transmembrane domain of hemagglutinin, I show that ten consecutive amino acids are required to confer resistance to detergent extraction. Although some of the …
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Functional and Structural Studies of the Human Voltage-Gated Proton Channel
… on the analysis of hHV1 by solution state NMR in detergent micelles. This technique allowed us to define the secondary structure of the channel for the first time but full three-dimensional structural characterization was determined to be unfeasible. From these studies, I conclude that the HV …
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Purification of the major envelope protein GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) from native virions
… proteins were solubilized with Triton X-100 detergent for further processing. GP5/M was not consistently identified in samples and was therefore abandoned. GP5 was identified by Western blot throughout processing with a αORF5 antibody. Cation exchange chromatography (CEX) was utilized for …
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Purification and characterization of the hepatic microsomal monooxygenase system from the coastal marine fish Stenotomus chrysops
… marine fish Stenotomus chrysops (scup) by detergent solubilization and column chromatography. Scup cytochromes P-450A, P-450B and P-450E (labeled in order of elution from the first ion exchange column) had distinct spectroscopic properties, substrate profiles and minimum molecular weights …