Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"desalting"”.
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Electrostatic control of microfluidic systems for enhancement of nanoparticle separations and FET nanobiosensors
… we demonstrate a method for localized electronic desalting of a FET biosensor. By locally removing the excess ions that shield the device from analytes, the Debye length around the senor can be sufficiently increased to encompass the molecular layers. Hence, the apparent charge of the target …
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Experimentation and application of directional solvent extraction for desalination of seawater and shale gas 'frac' flowback water
… for desalination of water has been tested for desalting seawater and shale gas 'frac' flowback water. The premise behind directional solvent extraction is that when certain organic oils such as decanoic acid are heated, they dissolve water without dissolving some other water soluble substances …
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An integrated process for the recovery of clinically significant trace proteins from human plasma
… and the performance of a new cellulose-based desalting medium was evaluated in comparison with ultrafiltration. A heat-treated specific immunoglobulin concentrate was prepared from side-stream fractions of an automated chromatographic process for the production of albumin concentrate, and a …
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Detection and enrichment of cytochrome P450s using bespoke affinity chromatography and proteomic techniques. Development of chemical immobilisation and novel affinity chromatography methods, with subsequent proteomic analysis, for the characterisation of cytochrome P450s important in cancer research.
… affinity chromatography, protein concentration/desalting, followed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and high performance liquid chromatography-mass spectrometry (HPLC-MS). A ketoconazole analogue and a 2-EN analogue, with known P450 inhibition, were …
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Electric Field Gradient Focusing-UV Detection for Protein Analysis
… for quantitative protein analysis. Simultaneous desalting and protein concentration as well as online concentration of ferritin and simultaneous removal of albumin from a sample matrix were also performed using this fiber-based EFGF system. In the approach of utilizing hydrogel-based EFGF, online …
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Molecular and physiological investigation of trace metal stress in seagrass, Zostera muelleri
… methods and proposed an optimised peptide desalting protocol. Additionally, major alterations in photosynthesis process as a result of Cu stress has led us to report on an optimised intact chloroplast isolation method that can be used for future proteomic-based studies.
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Identifying iron-binding antioxidants in New Zealand honeydew honey
… existed in the final fraction, even after desalting using reversed phase column chromatography. Due to that, other means of chromatography were trialled in order to obtain a salt-free fraction prior to mass spectrometry. The first procedure was modified by switching the sequence of the …
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Development of a rapid molecular method for detection of C. perfringens and other pathogens in water sources
… and purification methods (solid phase, desalting and magnetic bead purification) to obtain fit for purpose DNA from cultured C. perfringens. Extraction kits were also compared for equipment and infrastructure needed, efficiency of extraction, cost, and ease of use. All kits generated DNA …
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Engineering nanostructured selective layers for reverse osmosis membranes
… layers offers an attractive advantage in desalting applications where high flux is desirable, such as with brackish water resources as well as in membrane unit operations near their thermodynamic limit.
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Development and Applications of Liquid Sample Desorption Electrospray Ionization Mass Spectrometry (DESI-MS)
… pre-treatment such as LC separation, sample desalting, or sample clean-up processes, generating good sequence coverage of 97% and 52% for ubiquitin and BSA digestion, respectively. Also, biofluids such as untreated urine can be directly analyzed, demonstrating the high salt tolerance feature …
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Hyphenation of analytical techniques to single cell capillary electrophoresis-mass spectrometry
… interface. We addressed this by incorporating a desalting step into the sample preparation, which preserved metabolite material while precipitating interfering salts. We expect this addition to our CE–ESI-MS toolkit to enable greater metabolite detection and identifications in our single cell …
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Investigation of monolithic materials for protein sample preparation
… Although this method was shown to require sample desalting to remove denaturant (urea) and the performance of the fabricated monolith had low intra-chip reproducibility, the method was simple, reduced the risk of contamination, decreased the number of processing steps, and results in lower amounts …
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Novel molecular engineering approaches for genotyping and DNA sequencing
… silica particles as a stationary phase for desalting before MALDI-TOF analysis. By performing each functional step, we have demonstrated 100% on-chip single base incorporation, sufficient capture and release of the biotin-ddNTP terminated single base extension products, and high sample …