Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"dereplication"”.
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Metabolomics as a tool in the identification and production of new marine-derived antibiotics from sponges and endosymbiotic bacteria
… bacterium and its host sponge, as well as in the dereplication and metabolic profiling of other sponge-associated bacteria.24-Methylenecholesterol and two novel steroids, 24-vinyl-cholest-9-ene-3β,24-diol and 20-methyl-pregn-6-en-3β-ol, 5α,8α-epidioxy, all significantly active against Trypanosoma …
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Investigation of a deep-sea sediment-derived streptomyces coelicoflavus strain via genomic and metabolomic approaches
… περιβάλλον, δίνοντας έμφαση στο υψηλής απόδοσης dereplication και στον χαρακτηρισμό δομικά πολύπλοκων χρωστικών. Η γονιδιωματική ανάλυση αποκάλυψε ένα εκτεταμένο εύρος βιοσυνθετικών γονιδιακών συστάδων (BGCs), συμπεριλαμβανομένων πολυκετιδικών συνθασών (PKS), μη-ριβοσωμικών πεπτιδικών συνθετασών …
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Studies of Bioactive Natural Products and Mechanism-Based Bioassays
… activity in initial in vitro screens. A new dereplication method using aminopropyl SPE cartridges was applied to six of these extracts, and four of the extracts were dropped due to the presence of long-chain fatty acids (LCFAs). We present results for the testing and application of this …
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Investigating the chemical diversity and biomedicinal potential of South African actinomycetes for tuberculosis drug discovery
… prioritised for liquid-liquid partitioning and dereplication by LR LC-MS, followed by prefractionation and purification using a combination of HPLC and benchtop column chromatography. Structure elucidation of isolated compounds was achieved using NMR, LCMS and GC-MS data. Bioassays against M. …
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Isolation and characterisation of bioactive compounds from Vitex pinnata and associated fungal endophytes
… theobromae against Trypanosoma brucei brucei. Dereplication approach using metabolomics for identification of compounds from Pestalotiopsis olivacea was easily accomplished.
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Recovery and quality estimation of metagenomic assembled genomes of eukaryotes
… recovery process, I discuss how species-level dereplication for eukaryotes can be approached based on the genetic information alone. In summary I show that recovery of eukaryotic MAGs is a challenging but can be largely automated allowing large-scale studies to be performed.
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Medical Microbiology: Discovery of New Antimicrobial Drugs to Treat Clinically Relevant Antibiotic Resistant Pathogenic Bacteria
… a means to shortcut some of the more laborious dereplication methods and has facilitated rapid identification of novel antimicrobial agents. In addition, this work has provided further evidence indicating that bacteria from the deep sea could be a rich source of novel compounds. Further …
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Screening rare filamentous actinobacteria for novel antimycobacterial compounds
… Molecular networking allowed for the dereplication of known compounds early in the drug discovery process. Strains Amycolatopsis circi S1.3T , Actinomadura strain M2 grown in ISP2 medium and Kribbella speibonae SK5 produced active fractions. These compounds could not be matched to any …
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A path to biodiscovery: isolating Antarctic ascidian-associated bacteria and screening genomes for secondary metabolite-encoded biosynthetic gene clusters and signatures of cold adaptation
… we implemented a minimal media cultivation and dereplication strategy to isolate bacteria, rapidly screen for palmerolide A-encoding biosynthetic gene clusters using PCR and determine the identity of the novel isolates with long read 16S rRNA phylogenetic analyses. This work added phylogenetic …
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Exploitation of underused Streptomyces through a combined metabolomics-genomics workflow to enhance natural product diversity.
… agar and broth culture was used for metabolite dereplication and bioassays against multidrug resistant Acinetobacter baumannii, which is one of the bacteria on the World Health Organisation's list of pathogens that most urgently require new therapies. Fractions were derived from broth culture …
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Exploring the molecular diversity and biomedicinal potential of marine invertebrates and South African actinomycetes for tuberculosis drug discovery
… H37Rv. HR-LCMS analysis of the crude extract for dereplication suggested the presence of new compounds. A bioactivity, spectroscopy and spectrometry guided isolation procedure led to the isolation of three new natural products, a β-carboline alkaloid (1- …
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Genome-guided bioprospecting for novel antibiotic lead compounds.
… that can afford global visualisation and rapid dereplication of extract components, using tandem mass spectrometry fragmentation patterns of parent ions. This project provides an original and robust workflow that serves as a strong starting point in the isolation of novel β-lactone compounds …
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Genomics as a tool for natural product structure elucidation
… strain prioritization, novelty determination (dereplication), structure elucidation, three principal bottlenecks in the discovery process, as reviewed in Chapter 1. We report here progress in the use of genomics to facilitate the discovery and contextualization of new chemical matter. In …