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Showing 1 to 19 of 19 for “"deoxyribozyme"”.
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Increasing the thermostability of a light-producing deoxyribozyme
… the thermostability of the light-producing deoxyribozyme Supernova, a zinc-dependent catalyst of chemiluminescent reactions. At the structural level, sequence variants were designed by extending the triple-helix domain with different combinations of C·G·G and T·A·A triplets. In parallel, …
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Deoxyribozyme-Mediated Synthesis of Branched Nucleic Acids and Application to Modulation of Ribozyme Catalysis
Deoxyribozymes are single-stranded DNA molecules that catalyze a variety of chemical reactions. Catalytic DNAs have not been found in nature but can be identified by in vitro selection from random sequences. In this thesis, deoxyribozymes that ligate two RNA substrates or attach DNA to RNA are …
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Metal ion interactions with phosphorothioate and phosphoroselenoate DNA/RNA and biochemical characterization of a lead-dependent deoxyribozyme
Open Restriction set for Item 116982 on 2021-02-15T21:37:25Z with date null by madinag@illinois.edu.
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Deoxyribozyme-Mediated Synthesis of Branched and Lariat RNAs and Their Application in Studying Group II Intron's Splicing Mechanisms
… can be used for in vitro selection to identify deoxyribozymes with new activities.
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Deoxyribozymes to hydrolyze DNA phosphodiester bonds
… single-stranded DNA oligonucleotides are called deoxyribozymes. Up to now, there are no known deoxyribozymes in nature, and the only method to identify them is in vitro selection. Since the identification of the first deoxyribozyme in 1994, many deoxyribozymes have been found to catalyze various …
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DNA-catalyzed covalent modification of amino acid side chains in tethered and free peptide substrates
… evaluated and the first artificial DNA catalyst (deoxyribozyme) discovered in 1994. Since then, deoxyribozymes have been shown to catalyze a wide variety of chemical reactions, including phosphodiester bond cleavage, nucleic acid ligation, thymine dimer photoreversion, and the Diels-Alder …
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Deoxyribozymes That Prepare Branched and Linear RNA by Ligation
An ongoing challenge for deoxyribozyme-mediated RNA ligations has been synthesis of native 3'-5' phosphodiester linkages. Previously, reactions of 5'-triphosphate RNA have provided mostly 2',5'-branched RNA. Here we report two new in vitro selection strategies designed to favor formation of the …
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Deoxyribozymes for peptide substrates: exploring the landscape of nucleophiles and electrophiles
… catalyze various bioorganic reactions are called deoxyribozymes. Deoxyribozymes do not occur naturally and are identified using a combinatorial chemistry technique called in vitro selection. Since the discovery of the first artificial deoxyribozyme that catalyzes cleavage of phosphodiester bonds …
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Catalysis by DNA enzymes: roles of lanthanide(III) ions and DNA aptamer modules
… catalytic competence of DNA. Indeed, the first deoxyribozymes were reported in 1994. However, DNA enzymes are not found in nature and can only be identified from a pool of random sequences via an artificial selection process, referred to as the in vitro selection. DNA catalysts capable of …
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DNA as a Catalyst and as a Sensor: Investigating the Structure of the Ty1 Retrotransposition Intermediate and the Utility of DNA -Based Biosensors
… that can later be appended to an RNA-cleaving deoxyribozyme through a communication module, which can be rationally designed or identified by in vitro selection. Aptamers with high affinities (K d <2 microM) have been identified for estradiol (E2) and zearalenone (ZEN). Conversion of the …
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DNA enzymes for peptide-nucleic acid conjugation and for lysine methylation
… an essential role in many biological processes. Deoxyribozymes have been previously identified to join tyrosine-containing peptides to RNA by reaction of the tyrosine (Tyr) hydroxyl group with the a-phosphate of a 5′-triphosphorylated RNA oligonucleotide (5′-pppRNA). The selection approach for …
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Kinase deoxyribozymes
… with the substrates. Both ribozymes and deoxyribozymes have been identified to catalyze the phosphorylation of oligonucleotides. However, previous efforts to identify kinase deoxyribozymes to catalyze the phosphorylation of amino acid side chains were unsuccessful because the …
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DNA catalysts for amide bond cleavage and for lysine side chain modification
… can act as an efficient catalyst, the area of deoxyribozyme research is relatively unexplored, making the chances of discovering new deoxyribozymes likely. If deoxyribozymes can be identified that perform useful chemical reactions for which a catalyst has not yet been discovered, the impacts …
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Nucleobase-modified deoxyribozymes for amide and peptide hydrolysis
… catalysis. In fact, artificial DNA enzymes (deoxyribozymes) have been identified in laboratories by in vitro selection. The identification of new enzymes favors the use of nucleic acids over proteins for several reasons. First, nucleic acids can be amplified by natural enzymes, whereas …
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Search for new types of deoxyribozymes and development of human topoisomerases inhibitors on the basis of oligonucleotides
… on the search and confirmation of new types of deoxyribozymes that possess DNA self-cleaving activity. Certain guanine-rich oligonucleotides with potential to form G-quadruplexes were constructed and their self-cleaving activities are examined during our investigations. Our studies demonstrated …
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DNA enzymes for tyrosine PEGylation and azido-adenylylation of peptide and protein substrates
… be able to function as enzymes. DNA enzymes, or deoxyribozymes, have not been found in nature, but in vitro selection has led to the identification of deoxyribozymes for a variety of reactions. De novo enzyme identification favors the use of nucleic acids over proteins for several reasons. First, …
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In vitro selection of monovalent metal ion-dependent DNAzymes
DNA is no longer thought of as only the purveyor of genetic information, but also as a highly programmable and functional biopolymer. It was discovered in 1994 that some single-stranded DNA sequences can fold into complex tertiary structures to catalyze reactions, these DNA sequences are called …
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I. In vitro selection of aptamers for perchlorate and melamine II. Towards crystallization of DNAzymes III. A highly selective lead sensor based on a classic lead DNAzyme
Nature has given nucleic acids irreplaceable roles for all living creatures. It plays important roles in genetic information storage and transfer, as well as regulating various aspects of biology. The discovery of in vitro evolved functional nucleic acids opens a new field for nucleic acid …
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In vitro selection of DNAzymes selective for monovalent metal ions and their development as sensors in living cells and organisms
… and most interestingly, the catalytic function (deoxyribozymes or DNAzymes) of DNAs have been explored since 1990s, which attract widespread interest. DNAzymes, fully biocompatible but not naturally identified, commonly use metal ions as cofactors for catalysis, and some of them show exceptional …