Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"deoxycholate"”.
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Characterization of Deoxycholate-Responsive Genes Utilized by Brucella abortus 2308 During Oral Infection
… changes in B. abortus subjected to deoxycholate, a host bile acid encountered by bacteria during oral infection. Gene deletion strains of B. abortus were then created and tested for susceptibility to pH and bile acid stress, along with their ability to invade and replicate within …
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Wesselsbron virus : a biophysical, biochemical and serological study
… to the action of bother ether and sodium deoxycholate.
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Membrane reconstitution studies on the irreversibility of inactivation of sarcoplasmic reticulum of rabbit skeletal muscle
… sarcoplasmic reticulum vesicles by deoxycholate solubilisation and subsequent slow dialysis at room temperature. Reconstituted control sarcoplasmic reticulum had an average specific activity of 0,38 μmol calcium transported /minute /mg of protein. Acid-inactivated sarcoplasmic …
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Studies of the Host Response and the Induction of Immunosuppression in Murine Malaria
… fractions Peak I (C), Peak I (G), and the sodium deoxycholate void volume all act as a vaccine against Plasmodium infection and are all without immunosuppressive activity as assayed by the plaque technique. Immunosuppressive activity was found in association with the sedimented parasite fragments …
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Investigation of surfactant-drug pharmacokinetic interactions
… Tween® 20, Span® 20, Cremophor® CO40 and sodium deoxycholate. The surfactants were added at three different concentrations, 0.1%, 0.5% and 1.0% (w/v), to investigate if the addition of these surfactants had any influence on the membrane permeability of Rho123 and if these effects were …
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Antigenic and functional differences between the polar and lateral flagella of Azospirillum brasilense
… agents, p-nitrophenylglycerol, EDTA, sodium deoxycholate, sodium taurocholate, and Na₂SO₄, at appropriate concentrations could inhibit swarming without preventing growth. Based on the use of mutants lacking either the polar flagellum or the lateral flagella, the lateral flagella were found to …
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Adding Upstream Sequence and a Downstream Reporter to the Bile Acid Inducible Promoter of <I>CLOSTRIDIUM scindens</I> VPI 12708
… which form secondary bile acids (deoxycholate and lithocholate). Much research has illuminated the 7α-dehydroxylation pathway: of particular interest is the bile acid inducible operon, for which <i>Clostridium scindens </i>VPI 12708 serves as the model organism. There is a lack of …
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Evaluating Methods of Improving Recovery of Sub-lethally Injured Salmonella in Low Moisture Foods Treated with Antimicrobial Gas
… on the selective media Xylose Lysine Deoxycholate agar, while the average recovery on non-selective media was 4.50 log CFU/g and 5.74 log CFU/g from ClO2 and H2O2 treated LMFs, respectively. The use of non-selective media was correlated with increased recovery compared to selective …
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Solubilization and purification of aldolase from bovine sperm and muscle
… properties of BMA and BSpA were investigated. Deoxycholate, when included in homogenization buffer, solubilized approximately 81%, of the BMA per gram of tissue. Triton X100 in combination with phosphate buffer solubilized only 10% of the total 3SpA per cell. BMA was purified to greater than …
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The Effect of Environment on Virulence Factors and Resistance of Mucoid Strains of Pseudomonas Aeruginosa
… susceptibility to carbenicillin, polymyxin and deoxycholate with and without added magnesium. WT produced two major phenotypes which differed in colony size and appearance on nutrient agar. Both showed differences in the production of the virulence factors, protease, elastase, lipase, …
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Structural and functional studies of type three secretion virulence factors from gram-negative pathogenic bacteria
… the first step of TTS activation. The bile salt deoxycholate (DOC) binds to IpaD to induce recruitment of the first translocator, IpaB, into the maturing tip complex. The pore-forming translocators are bound by the class II chaperone, IpgC, within the bacterial cytoplasm in order to prevent …
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Aggregation Behavior of Chiral Single-Walled Carbon Nanotubes and their Transport in Landfill Conditions
… the most effective stabilizer followed by sodium deoxycholate (SDOCO) and sodium dodecyl sulfate (SDS). Molecular modeling was performed with chiral specific SWNTs and surfactants to better understand their interaction behavior which provided insight through interaction energy and conformational …
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Investigating the effect of mutations in lipopolysaccharides (LPS) (O-antigen and Core region) of Salmonella Typhimurium on the colonisation of “microgreens”
… ATCC 14028 were plated on Xylose Lysine Deoxycholate (XLD) agar to quantify bacterial proliferation and attachment. These results demonstrated that S. Typhimurium ATCC 14028 successfully colonised the roots of all nine microgreens, with higher bacterial counts observed in rhubarb chard, …
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Isolation, growth and epidemiology of campylobacter jejuni / coli
… by incorporating cephazolin (10 mg/i) and sodium deoxycholate (0.1%). This medium and the SBA gave similar isolation rates from faecal specimens when incubated at 42 0C for 42h but the BFSA was less selective. A blood-free selective enrichment broth (BFSEB) containing sodium metabisulphite (0.05%) …
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CHEMICAL AND PHYSICAL CHARACTERIZATION OF COMPLEX III; THE OXIDATIVE REACTION MECHANISM
… of pH when titrations were performed in deoxycholate buffers, while a variation of -30 mV per pH unit was observed for both b species in taurocholate buffers. These two detergents also produced different MCD-contributions of the two b hemes. The oxidative reaction mechanism was …
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Evaluating the Persistence of Salmonella Tennessee and Lactobacillus brevis in Non-Alcoholic Beer under Low Pasteurization
… duplicate on tryptic soy agar and xylose lysine deoxycholate agar at 0, 1, 7, 14, 31, 59, 99, 129, 150, and 183 days, and co-inoculation samples were additionally inoculated on universal beer agar with 0.1% cycloheximide. PCR was used to confirm presumptive Salmonella positives. Data were …
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Enterobacterial Common Antigen Biosynthesis in Shigella flexneri
… alternative sensitivities to Colicin E2 and deoxycholate. I ultimately determined that this was due to the regulatory disruption of the adjacent gene, wecG, in one of the mutants and highlighted WecG’s importance in the biosynthetic pathway. Subsequently I investigated WecG which, like WzyE, …
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Expression of the multi-drug efflux genes acrAB of Escherichia coli
… 0.5 M sodium chloride, bile salts (cholate and deoxycholate), ampicillin, and growth on certain carbon sources, were found to induce acrAB transcription. A family of isogenic mutant strains based on the lacZ derivative of E. co/i MG1655 (SD 1648) harbouring specific gene disruptions was …
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Physicochemical studies of a novel adjuvant and conjugate vaccines
… phase extraction (SPE), acid precipitation using deoxycholate (DOC/HCI) and ultrafiltration (UF). At physiological pH, the binding capacity was low and so the SPE method was not investigated further. For Mn A-IT the DOC/HCI method generally gave lower free saccharide values than the UF method; …
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Phenotypic and genotypic characterization of salmonella isolates from chickens and humans in South Sudan.
… The samples were cultured on Xylose Lysine Deoxycholate Agar for the isolation of Salmonella and further confirmed using a series of biochemical tests and PCR. Following confirmation of Salmonella, the phenotypic and genotypic characterization was done. Phenotypic characterization was done …
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