Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 39 for “"denaturing gradient gel electrophoresis"”.
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Identification of The Causative Bacteria In Musculoskeletal Infections Using 16S Rdna - Denaturing Gradient Gel Electrophoresis Analysis
… One way to solve this problem is to use denaturing gradient gel electrophoresis (DGGE) to separate the PCR products before DNA sequencing. Denaturing gradient gel electrophoresis (DGGE) separates DNA molecules based on their melting point, which is determined by their DNA sequence. This …
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A polymerase chain reaction and denaturing gradient gel electrophoresis procedure for analysis of arbuscular mycorrhizal fungi in soil
… In this study, a polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE) procedure for the detection of fungal 18S ribosomal RNA gene was developed with reference cultures. Five AMF species were procured from the International Culture Collection of Arbuscular and …
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Characterization of prokaryotic diversity in a chlorinated solvent-contaminated bedrock aquifer using denaturing gradient gel electrophoresis, ribosomal DNA sequencing, and real time PCR
… of the samples were generated by the use of denaturing gradient gel electrophoresis (DGGE), and were analyzed to detect spatial and seasonal changes. DGGE fingerprints revealed significant compositional differences between the attached and the unattached microbial communities. Cluster …
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An integrated geochemical and microbiological investigation of sulphate reduction in hypersaline pans
… of the microbial community was studied by denaturing gradient gel electrophoresis, from which bands were excised for DNA sequencing. Denaturing gradient gel electrophoresis patterns were analysed statistically by cluster analysis and principal components analysis. High in situ rates of …
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Detection of an Introduced Bacterial Culture in Greywater Treatment Reactors
… rRNA gene by polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) revealed community profiles of the dominant bacterial species. Species and strainspecific primers targeting either the 16S rRNA gene or the intergemc spacer (ITS) between the 16S and 23S rRNA genes were …
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Influence of Petroleum Deposit Geometry on Local Gradient of Electron Acceptors and Microbial Catabolic Potential
… the effect of oil deposit geometry on localized gradients of electron acceptors and microbial community composition, factors that are critical to accurately estimating biodegradation rates. One-dimensional top-flow sand columns with 12-hour simulated tidal cycles compared two contrasting …
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Caratterizzazione Genotipica e Fenotipica di Ceppi di L. rhamnosus di Origine Umana e Alimentare
… of culture-independent methods such as Denaturing Gradient Gel Electrophoresis (DGGE), gives the opportunity to trace the evolution of lactic acid microflora during the ripening of these traditional foods. Next to this, physical and chemical analyses have been integrated in order to …
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Improving Our Comprehension of Microbial Communities
… ratios. Other SSU rRNA-based studies, such as denaturing gradient gel electrophoresis (DGGE), have been used as an alternative to SSU rRNA gene sequencing because they can provide many community profiles for a fraction of the time, effort and money required for sequencing. Chapter 3 provides a …
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PCR-DDGE Comparison of Fecal Bacteria From Captive and Wild Populations of the Endangered San Esteban Island Chuckwalla (Sauromalus varius)
… 16S rDNA polymerase chain reaction-based denaturing gradient gel electrophoresis (DGGE), which overcomes many limitations of indirect microbiological methods, was used in this study. The banding patterns produced suggest that the wild and captive-bred lizards have different bacterial …
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Gut-associated microbial symbionts of the marsh fiddler crab, Uca pugnax
… among individuals and over time, as assessed by denaturing gradient gel electrophoresis (DGGE). Community composition, however, does differ between stomach and hindgut populations, as resolved by DGGE and clone libraries of the 16S rRNA gene. Many recovered clones were most closely related to …
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Characterization of Microbial Survivall in the Presence of Radioactive 14C Spiked Salts in Order to Minimize W,aste from Graphite Moderated Nuclear Reactors
… well. The bacteria samples were analysed using Denaturing Gradient Gel Electrophoresis (DGGE). The objective was to determine the diversity of the bacteria thriving in radioactive carbon species. The results obtained from the analyses were as follows; SEM results indicated that bio-film is …
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Interactions between salmonella typhimurium and human gut bacteria
… profiling of faecal bacteria measured by denaturing gradient gel electrophoresis did not show any change specifically associated to S. Typhimurium inactivation. It was not possible to identify the bacterial strains responsible for the inactivation of S. Typhimurium; however, this effect …
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Microbial Ecology of the Porcine Gastrointestinal Tract in Relation to Lactobacillus Reuteri MM53 and Bacitracin Treatments
… bacitracin additions using molecular techniques. Denaturing gradient gel electrophoresis was used to monitor bacterial population changes and these changes were assayed based upon shifts in fecal banding patterns. Inclusion of antibiotics in the diet caused a simplification of V3-16S rDNA banding …
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Assessment of flagellate diversity at deep-sea hydrothermal vents using the combined approach of culture-dependent and culture-independent methods
Eighteen strains of flagellated protists representing 9 species were isolated and cultured from four deep-sea hydrothermal vents in the Eastern Pacific Ocean: Juan de Fuca Ridge, Guaymas Basin, and both 21°N and 9°N on the East Pacific Rise (EPR). The hydrothermal vent flagellates belonged to six …
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Molecular biology approach to anaerobic digestion of marine biomass: Anaerobic digestion from marine materials. What makes a methanogen happy?
… A number of molecular techniques including, denaturing gradient gel electrophoresis, quantitative polymerase chain reaction, cloning and sequencing were employed to study the microbial ecology of the seaweed fed reactors. Results show that marine sediment is a viable microbial source for …
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Aspects of functional constipation - a trial of an opioid antagonist as a treatment and an investigation of the colonic microbiota associated with the illness
… without FC. Faecal samples were analysed using denaturing gradient gel electrophoresis (DGGE), fluorescence in situ hybridisation (FISH), and pyrosequencing. RESULTS 41 females were recruited (median age 45, range 23-76 years) .There were no significant differences in ‘satisfactory improvement …
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The effect of biostimulation on geochemical and microbiological conditions in an isolated dolostone fracture
… were also observed through 16S rDNA analysis. Denaturing gradient gel electrophoresis results indicated substantial diversification and growth of the microbial community following biostimulation. Further research will investigate the potential for bioclogging at a NAPL-contaminated fractured …
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The Utility of Culture Independent Methods to Evaluate the Fecal Microbiome in Overweight Horses Fed Orchard Grass Hay
… fed grass hay. In the first study, eight Arabian geldings limit-fed an 18% vs. 12% non-structural carbohydrate (NSC) hays in a cross-over design during two 28-day periods were included to evaluate the influence of grass hay NSC on serum insulin and plasma glucose concentrations. Serum insulin …
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An Analysis of Mitochondrial DNA in Rett Syndrome and Other Neurodegenerative Disorders
… large deletions, or insertions, have been largely neuromuscular in origin. However, as an understanding of the effects of mtDNA mutations progresses, attention is now focusing on neurodegenerative diseases. Rett Syndrome (RS), a progressive neurodegenerative disease with predominantly female …
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Bacterioplankton in the Chesapeake Bay: Genetic diversity, population dynamics and community proteomics
… populations were monitored from 2002 to 2004 by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA gene fragments. Remarkable seasonal shifts and repeatable annual patterns were identified. Temporal variation of bacterial communities was best explained by the change of …
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