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Showing 1 to 5 of 5 for “"denatured states"”.
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Structural and Biophysical Characterisation of Denatured States and Reversible Unfolding of Sensory Rhodopsin II
… 2 investigates the structural features of SDS-denatured states and the kinetics for reversible unfolding of sensory rhodopsin II (pSRII), a retinal-binding photophobic receptor from Natronomonas pharaonis. pSRII is difficult to denature, and only SDS can dislodge the retinal chromophore without …
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NMR study of the unfolding of ribonuclease A, and dynamical studies of liquids in confined geometries
… method. It was found that the pressure denatured states of RNase A display some characteristics of a molten globule, and all three $\alpha$-helices and the $\beta$-sheet of the native protein remain partially folded structures in the pressure denatured state. $\sp1$H spectra of the …
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Investigation of the interaction of GdnHCl with poly-L-lysine, horseradish peroxidase and cytochrome c monitored via FTIR, CD and fluorescence spectroscopies
… (HRP) and cytochrome c. Probing the chemically denatured states of proteins by FTIR is a novel technique, which has only recently been reported. To aid in the interpretation of the FTIR results, fluorescence and CD were employed. The use of deuterated solvents, i.e. Gdn-d 5 -DCl and D 2 O, …
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Analysis of the thermodynamic determinants of protein fold specificity in the denatured state ensemble
… becoming clear that focusing only on the native states of protein folds will be insufficient for deciphering the protein folding problem. Knowledge of the thermodynamics of the denatured state is also necessary. In this project, the thermodynamic determinants of the native fold, present in the …
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Computational investigations of protein dynamics and its implications for biological functions
… carried out on the λ*YG mutant. High-pressure denatured states are found to contain a significant amount of helical structure. Upon pressure drop, the protein refolds into the native state in 20 μs. The simulations confirm the existence of pressure-jump induced fast folding pathway for λ*YG. We …