Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 108 for “"denatured"”.
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Hydrogen bonding and solvation dynamics of n-methylacetamide in denatured water (D₂O) or denatured chloroform (CDCl₃) from nonlinear spectroscopy
Hydrogen bonding between N-methylacetamide (NMA) and different solvents (D₂O or CDCl₃) was studied by using two-dimensional infrared spectroscopy to probe the frequency fluctuations of the amide I mode of the solvated NMA. An iterative fitting approach was used to extract a correlation function …
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Self-protection analysis of denatured thorium-plutonium fuel
… The U-232 dose rate was also calculated for the denatured case. Ultimately, the study found that there was a negligible different in the amount of time that it takes for either fuel type to become self-protective. The denatured case showed that it requires much more plutonium than the undenatured …
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Computational Prediction of the Agregated Structure of Denatured Lysozyme
… et al. (2008) observed conditions where heat denatured lysozyme formed fibril structures that were observed to be 8-17 nanometers in diameter under the electron microscope. Even though the crystal structure of lysozyme is known, the denatured form of this protein is still unknown. Therefore, …
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Thermodynamics and Kinetics of Iso-1-cytochrome c Denatured State
… a system of studying simple loops in the denatured state using c-type cytochromes. New insights into how the properties of these loops impact the denatured state are outlined in this thesis. First, studies on a 22-residue loop revealed a previously unreported finding that equilibrium loop …
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Thermodynamics and Kinetics of Iso-1-cytochrome c Denatured State
… a system of studying simple loops in the denatured state using c-type cytochromes. New insights into how the properties of these loops impact the denatured state are outlined in this thesis. First, studies on a 22-residue loop revealed a previously unreported finding that equilibrium loop …
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NMR Investigations of Structure in Pressure and Cold Denatured Proteins
Because of the extreme temperatures and pressures used to perform the cold denaturation studies, hydrogen exchange rates in model amides were measured at various temperatures and pressures to determine the effect of the extreme conditions. These reaction rates are compared to those obtained by …
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Structural Comparison of Denatured and Supercharged Proteins in Gas and Solution Phases
… on non-native proteins. This work analyzes denatured and supercharged proteins using different mass spectrometry techniques to compare the solution and gas phase structures. First, measuring the amount of unfolding under various denaturing and instrument conditions helps to identify how …
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Evaluation of gasoline-denatured ethanol as a carbon source for wastewater denitrification
… rates. This study introduces gasoline-denatured ethanol (dEtOH), which is now being produced in large quantities for the production of E10 gasoline, as an alternative carbon source. The gasoline added, as the denaturant, is known as "straight-run" gasoline; a lower grade material that …
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Analysis of the thermodynamic determinants of protein fold specificity in the denatured state ensemble
… problem. Knowledge of the thermodynamics of the denatured state is also necessary. In this project, the thermodynamic determinants of the native fold, present in the denatured ensemble, were investigated and the critical role of the denatured state ensemble in controlling protein folding is …
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Structural and Biophysical Characterisation of Denatured States and Reversible Unfolding of Sensory Rhodopsin II
… 2 investigates the structural features of SDS-denatured states and the kinetics for reversible unfolding of sensory rhodopsin II (pSRII), a retinal-binding photophobic receptor from Natronomonas pharaonis. pSRII is difficult to denature, and only SDS can dislodge the retinal chromophore without …
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THERMODYNAMIC AND KINETIC PROPERTIES OF CYTOCHROMES C AND C’ IN THE DENATURED STATE: PROPENSITY FOR RESIDUAL STRUCTURE
… helical propensity and residual structure in the denatured state, two approaches have been taken. In the first project, we have engineered serine in place of alanine near the center of the third helix (positions 83 and 87) in cytochrome c’ (Cytc’) and have measured histidine-heme loop formation in …
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THERMODYNAMIC AND KINETIC PROPERTIES OF CYTOCHROMES C AND C’ IN THE DENATURED STATE: PROPENSITY FOR RESIDUAL STRUCTURE
… helical propensity and residual structure in the denatured state, two approaches have been taken. In the first project, we have engineered serine in place of alanine near the center of the third helix (positions 83 and 87) in cytochrome c’ (Cytc’) and have measured histidine-heme loop formation in …
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NMR study of the unfolding of ribonuclease A, and dynamical studies of liquids in confined geometries
… method. It was found that the pressure denatured states of RNase A display some characteristics of a molten globule, and all three $\alpha$-helices and the $\beta$-sheet of the native protein remain partially folded structures in the pressure denatured state. $\sp1$H spectra of the …
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Chemical and physical changes resulting from UHT treatment of milk
… rennin coagulation of the caseins and the heat denatured serum proteins. A fluorometric method of analysis for total sulfhydryl content of milk serum was developed that was rapid and sensitive to changes in parts per billion. Quantitative changes in total sulfhydryl at the different ultra-high …
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Investigation of the interaction of GdnHCl with poly-L-lysine, horseradish peroxidase and cytochrome c monitored via FTIR, CD and fluorescence spectroscopies
… (HRP) and cytochrome c. Probing the chemically denatured states of proteins by FTIR is a novel technique, which has only recently been reported. To aid in the interpretation of the FTIR results, fluorescence and CD were employed. The use of deuterated solvents, i.e. Gdn-d 5 -DCl and D 2 O, …
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Early Events in the Folding of the Protein Ubiquitin
… In the case of the T-Jump, initially cold denatured protein solution is rapidly heated with a 20 nsec heating pulse. The subsequent refolding is monitored by time resolved fluorescence lifetime and spectrum. In the case of stopped flow mixing, an initially chemically denatured protein …
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High-pressure NMR studies of isomerizational exchange rates in liquids
… resonance in both the native and denatured forms. The data indicated the possibility of a second major denatured structure under these conditions, while thermal denaturation techniques indicated only one major denatured structure. Individual rate constants of folding and unfolding …
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Albumin Adsorption: Inferences of Protein Interactions Measured by Sedimentation both Between Species and Induced by Denaturing
… velocity to those in the reversibly denatured BSA experiments. Average aggregate size for reversibly denatured BSA was well within the ranges found for non-denatured BSA. In conclusion, irreversibly denatured BSA formed larger aggregates and was more likely to desorb from the …
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Analysis of the interaction between the co-chaperone p23 and the aryl hydrocarbon receptor
… activation domain was cloned, purified in denatured conditions from bacteria, refolded via limited dialysis, and analyzed for proper refolding via co-immunoprecipitation with the known binding partner SRC-1. This AhR NΔ515 transactivation domain construct was used, along with amino terminal …
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Nucleic acid denaturation at an electrode surface
… at a nano-structured gold electrode and then denatured into its constituent components by driving the potential at the surface cathodic (electrochemical melting). This denaturation is typically monitored by observing changes in the SERS spectra of a reporter molecule attached to one of the two …
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