Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 94 for “"deletion mutants"”.
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Investigating Epigenetics as a Mechanism to Regulate Bacterial Heterologous Expression
… by obtaining and comparing DNA methyltransferase deletion mutants in a variety of bacterial species that have been widely used as heterologous hosts, namely gram-positive Bacillus subtilis, and gram-negative Escherichia coli and Pseudomonas putida. For the gram-negative strains, we confirmed the …
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Genetic and Biochemical Characterization of Methanol Utilization in Methanosarcina Acetivorans C2A: Analysis of the Methanol:methyl-Coenzyme M Methyltransferase System Encoded by mtaCB and mtaA
… a markerless exchange method was developed and deletion mutants were constructed which lacked one or more mtaCB or mtaA isozymes. None of the mtaCB or mtaA deletion mutants was affected in trimethylamine and acetate metabolism indicating they have no role in the utilization of these substrates. …
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Enzymatic and Splicing Activity of Class I Leucyl -Trna Synthetases From Escherichia Coli and Saccharomyces Cerevisiae
… into a competent ternary splicing complex. RNA deletion mutants have been quantitated for effects on complex assembly and splicing activity.
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Identification and characterization of the inlGHE gene cluster of Listeria monocytogenes
… recombination leading to an in-frame deletion which removed the 3'-terminal end of inlC2 and a 5'-portion of inlD. The genes inlG, inlH and inlE seem to be transcribed extracellularly and independent of PrfA. To study the function of the inlGHE gene cluster several in-frame deletion …
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Regulation of Skeletal Muscle Differentiation by a Rapamycin -Sensitive Signaling Pathway
… mTOR was examined by a series of truncation and deletion mutants. Strikingly, the results indicate that the C-terminal 1187 amino acids are sufficient for mTOR's myogenic function and the N-terminal half of the protein is completely dispensable. Both the FKBP 12-rapamycin binding domain (FRB) and …
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Understanding the Role of the Hypervariable Region in the Open Reading Frame 1 of the Hepatitis E virus in Viral Replication
… region. Initially we constructed two partial deletion mutants of genotype 1 human HEV, hHVRd1 and hHVRd2, with in-frame deletion of amino acids (aa) 711 to 777 and 747 to 761 in the HVR of a sub-genomic GFP HEV replicon. Expression of enhanced green fluorescent protein by the mutant hHVRd2 …
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Characterization of the Bacillus anthracis SleL Protein and its Role in Spore Germination
… methods. In this work we created in-frame deletion mutants to study not only the role of one GSLE, SleL, but by creating multi-deletion mutants, we were able to analyze how the protein cooperates with other lytic enzymes to efficiently hydrolyze the cortical PG. We determined that SleL …
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Comparison of induced proteins synthesized in hydrogen peroxide and near-ultraviolet -treated bacterial cells
… coli and Salmonella typhimurium wildtype, mutants constitutively overexpressing proteins controlled by the oxyR regulon, and deletion mutants of oxyR. In the oxyR mutants that overexpress specific proteins constitutively, 11 newly synthesized proteins were induced by H2 O2 in S. typhimurium …
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Analysis of a trichomonas vaginalis cysteine protease
… which is a characteristic of lysosomes. Lastly, deletion mutants of CP1 were created to determine the role of the prodomain in targeting CP1 to vesicles.</p>
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Pilus biogenesis in uropathogenic Escherichia coli : An electrophysiology study on the PapC usher
… domains on the usher channel behavior. Domain deletion mutants characterized using planar lipid bilayer showed the modulating effects of the α-helix, plug, N- and C-terminal domains on the usher channel behavior. In contrast to the wild type, domain deletion mutants displayed open channel …
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Role of the Small Terminase Subunit Encoded by Staphylococcus Aureus Pathogenicity Island SaPI1 in Formation of SaPI1 Transducing Particles
… hypothesis. Induction of the 80α small terminase deletion mutant produces wild type levels of SaPI1 transducing particles, demonstrating that SaPI1 small terminase can replace that of 80a in SaPI1 packaging. Southern blot analysis of virion DNAs isolated from the deletion mutants confirms that …
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Characterization of cpeY and cpeZ mutants in Fremyella diplosiphon strain UTEX 481
… PE purified from <em>cpeY</em> and <em>cpeZ</em> deletion mutants and compared them with wild type (WT). Both <em>cpeY</em> and <em>cpeZ </em>mutants generated much less PE than WT as well as assembling much less PE into the PBS. PE purified from <em>cpeY</em> mutant had phycocyanobilin on …
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Strategies to potentiate bactericidal antimicrobial activity based on the suppression of bacterial stress response systems
… exposure; and (iii) to determine the impact of deletion of the recA gene (the SOS response activator, also required for homologous recombination in E. coli) on the reversal of heteroresistance. For the first and second objectives, a set of E. coli mutants was prepared. In E. coli BW25113, these …
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Regulation of mRNA expression through 3’ UTR sequences of IL6 gene in amino acid-deprived HeLa cells
… seemed to be broadly distributed according to deletion mutants studies of IL6 3’UTR. NF-κB functioned in 3’UTR to partially contribute to stabilization of IL6 mRNA according to knock-down experiments of p65, NF-κB subunit. Finally, treatments of p38 MAP kinase inhibitor, SB203580 markedly …
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Structural Characterization of the C-terminus of the Cochlear Motor Protein Prestin: Intrinsic Disorder Linked to Voltage Sensor Function
… the protein’s voltage sensor. The fact that the deletion protein exhibited NLC implies that the mutation did not adversely affect the structure. It was hypothesized that only a small subset of residues, attributed to the STAS domain, contribute to the structure in prestin’s C-terminus. To …
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Studying the riboregulatory role of Hfq in Klebsiella pneumoniae using CLASH (crosslinking, ligation and sequencing of hybrids)
… mrdA (encodes Penicillin Binding Protein 2). Spf deletion mutants exhibited decreased susceptibilities to β-lactam antibiotics like ampicillin, carbenicillin, benzylpenicillin, mecillinam, and penicillin V. Additionally, OmrA/B and RyhB sRNAs were involved in regulating the antibiotic entry of new …
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Activities of Bacteroides Conjugative Transposons: CTnGERM1 and CTnDOT
… supported by results from experiments involving deletion mutants.
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The Interaction Between Eukaryotic Translation Initiation Factor eIF4G and 3’ Cap Independent Translation Element of Barley Yellow Dwarf Virus Is Affected by Multiple Initiation Factors
… translation are still unclear. Three eIF4G deletion mutants with different domain organization were used to investigate BTE interaction with eIF4G: eIF4G601-1196 is the eIF4G fragment containing amino acid residues from 601 to 1196, including binding domains for eIF4E, central eIF4A, eIF4B …
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Cadherin recognition and specificity
… biphasic kinetics. Further studies with domain deletion mutants showed that the EC1 domain of cadherin extracellular region is responsible for the first phase of the biphasic kinetic profile. The affinity and dissociation rate of EC1 bond were then extracted by modeling the first phase. Second, …
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Use of an Inducible Promoter to Characterize Type IV Pili Homologues in Clostridium perfringens
… mutation system to create in-frame deletion mutants in three genes with homology to Type IV pilins, and we used the promoter system described above to complement the mutants. We analyzed each pilin for localization and expression, as well as tested each of the mutants for various …
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