Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"deletion construct"”.
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Bacterial expression of radio-labeled recombinant proteins for studying AHR signalling
… the production of Ara-9 and C∆418 (an ARNT deletion construct) in a modified thioredoxin fusion system. These proteins were expressed in a system that allowed for removal from the fusion partner via a thrombin recognition site as well as the incorporation of an in vitro phosphorylation site. …
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Die Rolle der Janus-Kinasen-spezifischen Insertion (JSI) in JAK2
… of JAK2 by its PseudoKinase domain. We cloned a deletion construct (delta JSI) as well as different point mutations of JAK2 (F1061A, M1062A, M1064A, I1065A). In the further process it showed up that the constructs F1061A, M1064A and I1065A had lost their Kinase activities by the mutations. …
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Design and synthesis of aryl hydrocarbon receptor fusion proteins for polyclonal antibodies production and cellular delivery
… and synthesis of a number of proteins of the AHR deletion construct CΔ553. The bacterial expressed and purified fusion proteins could be utilized as antigen to generate antibodies and be used for cellular delivery. The purified protein was immunogenic in rabbits and produced significant …
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Subzelluläre Lokalisation des Y-box-Proteins-1 (YB-1) in Mesangialzellen und Charakterisierung der dafür verantwortlichen Proteindomänen
… translocated into the nucleus. Cloning various deletion construct of YB-1 with a fluorescent tag we identified three nuclear localisation signals (NLS: AS 185-194, AS 149-156, AS 276-292) and two cytoplasmic localisation signals or nuclear export signals ( ZLS: AS 1-57, AS 52-146). Experiments …
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Isolation and characterization of latex-specific promoters from Papaver somniferum L.
… the functional sites within the DIOX promoter, deletions were made 1.5 kb and 0.14 kb upstream of the ATG start codon, fused to GUS, and transformed into opium poppy, Arabidopsis thaliana, and tobacco (Nicotiana tabacum). The 2.5 kb DIOX:GUS and 1.5 kb EcoRIDIOX:GUS reporter gene constructs …
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Identifizierung und Kartierung von nukleären Import- und Exportsignalmotiven innerhalb des Y-box-bindenden Proteins-1 (YB-1)
… export sequences of YB-1 protein, various YB-1 deletion constructs and stimulations were used in different cell lines. Mutation studies showed that both Nuclear Localisation Sequences (NLS) of the bipartite NLS 276-292 are required for nuclear localisation. On the other hand, the length of the …