Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 53 for “"dehydratase"”.
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Characterization of six Arabidopsis AROGENATE DEHYDRATASE promoters
… is catalyzed by an enzyme called AROGENATE DEHYDRATASE, of which there are six different isoenzymes encoded by the Arabidopsis genome. All six have specialized roles within the plant, and are differentially expressed during development and under stressful conditions. To deduce the …
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Mining the enolase superfamily for new functions: investigations of D-glucarate dehydratase related proteins (GlucDRP) and L-lyxonate dehydratase proteins (LyxD)
… an enolase superfamily member: D- glucarate dehydratase related protein (GlucDRP). GlucDRP share more than 60% sequence identity with GlucD, a well-characterized protein. I showed that there is a protein-protein interaction between GlucD and GlucDRP which form a detectable heterospecies. The …
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Galactonate Dehydratase: Exploring the Frontiers of the Enolase Superfamily
The crystal structure of GalD indicates that His 185 may serve as a general acid catalyst to facilitate the departure of the 3-OH leaving group and/or stereospecifically protonate the product in the subsequent tautomerization reaction. The function of His 185 was investigated by comparing the …
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Strukturelle Untersuchungen an dem Radikalenzym (R)-2-Hydroxyisocaproyl-CoA-Dehydratase
… katalysiert. Die (R)-2-Hydroxyisocaproyl-CoA-Dehydratase katalysiert die Dehydratisierung von (R)-2-Hydroxyisocaproyl-CoA in der Fermentation von L-Leucin im humanpathogenen Bakterium Clostridium difficile. Im Gegensatz zu einer Vielzahl von Radikalenzymen, wie beispielsweise der Coenzym …
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Functional and physiological discovery in the mannonate dehydratase subgroup of the enolase superfamily
… physiological characterization of the mannonate dehydratase subgroup (ManD) of the enolase superfamily (ENS). The outcome affirms the dangers of homology-based annotations while discovering novel metabolic pathways. Furthermore, the experimental verification of these pathways (in vitro and in …
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Functional Analysis of Dehydratase Domains of a Polyunsaturated fatty acid Synthase from Thraustochytrium sp. by Mutagenesis
… each with multiple catalytic domains. Three dehydratases (DH) domains in the PUFA synthase are believed to be responsible for coordinately introducing multiple double bonds in VLCPUFAs; however, the exact function of these domains remains to be determined. In this research, two DH domains …
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Biochemical characterization of Cj 1123 c, a putative acetyltransferase & Cj 1319, a putative C6 dehydratase from Campylobacter jejuni.
… and Cj 1319, a putative GDP-mannose dehydratase encoded by the N- and O-linked protein glycosylation loci in C. jejuni, respectively. We show that Cjll23c is responsible for the synthesis of UDP- diacetamidobacillosamine. We also show that Cj 1123c can N-acetylate the O- glycosylation …
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Functional assignments in the enolase superfamily: investigations of two divergent groups of D-galacturonate dehydratases and galactarate dehydratase-III
… the Microscilla group of D-galacturonate dehydratases, the Geobacillus group of D-galacturonate dehydratases, and galactarate dehydratase-III from Agrobacterium tumefaciens strain C58. Although the two groups of D-galacturonate dehydratases produce an identical product, they act through …
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Evaluation of erythrocyte amino levulinic acid dehydratase as an indicator of chronic lead exposure in wild populations of rainbow trout
The activity of erythrocyte amino levulenic acid dehydratase (ALA-D) and liver and bone lead concentrations were measured in 141 wild rainbow trout from two highway-influenced and two pristine streams. A significant relationship between ALA-D activity and liver lead concentrations among streams (r …
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Untersuchungen zur Inaktivierung und Reaktivierung der Coenzym B12-abhaengigen Glycerin-Dehydratasen aus Citrobacter freundii und Clostridium pasteurianum
Die Coenzym B12 abhaengige Glycerin-Dehydratase aus Citrobacter freundii ist an der anaeroben Umsetzung von Glycerin involviert. Sie katalysiert die Dehydratisierung von Glycerin zu beta-Hydroxypropionaldehyd. Das Enzym unterliegt einer sogenannten ''Suicide''-Inaktivierung durch eben dieses S! …
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Investigation of factors influencing the d-Aminolevulinic acid dehydratase assay of blood-lead with a specific reference to the in vitro effects of monovalent cations
… of factors influencing the δ-aminolevulinic acid dehydratase (ALAD) for monitoring lead-blood concentration was performed with a view towards elimination of procedural artifacts. Using the optimum procedure the in vitro effects of the monovalent cations of Li, Na, K, Cs, NH4, Tl, Hg, and Ag on the …
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Mechanistic Investigations of Mandelate Racemase: The Electrophilic Catalysts
… muconate lactonizing enzyme (MLE), galactonate dehydratase (GalD) and glucarate dehydratase (GlucD) were determined. MR and MLE bind and require a single metal ion for activity. GalD and GlucD require two metals for activity and show inhibition at high metal ion concentrations with Mn$\sp{2+}.$.
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Konstruktion und Durchmusterung von Metagenombanken: Identifizierung und Charakterisierung von Genen und Genprodukten von am Polyol-Stoffwechsel beteiligten Oxidoreduktasen und Coenzym B12-abhängigen Dehydratasen
… Gene für Koenzym B12-abhängige Glycerin-und Diol-Dehydratasen wurden vier sogenannte Metagenom-Banken hergestellt. Als Proben wurde Boden eines Zuckerrübenfeldes, Sediment des Flusses Grone (beide nahe Göttingen, Deutschland), Sediment des Solar Lake (Ägypten) und Sediment des Golfs von Eilat …
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Biosynthesis of the antimicrobial peptide epilancin 15X
… position of the core peptide by a lantibiotic dehydratase (ElxB), followed by proteolytic removal of the leader peptide by a serine-type protease (ElxP), and hydrolysis of the resulting N-terminal dehydroalanine residue. A pyruvyl group thus formed is finally reduced to Lac by a dehydrogenase …
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The Roles of Peroxynitrite and Superoxide in Oxidative Damage to E. Coli
… superoxide. Of nineteen enzymes tested, only the dehydratase enzymes containing these iron-sulfur clusters were significantly affected by peroxynitrite challenge. These iron-sulfur clusters could be repaired, and both iron storage proteins and iron import seem to be necessary for efficient repair. …
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Amino Acid Biosynthesis and the COP9 Signalosome in Aspergillus nidulans
… identifiziert. Die Imidazol Glycerolphophat Dehydratase HISB (E.C. 4.2.1.19) ist essentiell für die Histidin Biosynthese. Ein hisB-Deletionsstamm wächst wenn geringe Histidinmengen supplementiert werden, anders als beim Wildtyp zeigt er unter diesen Bedingungen jedoch eine aktivierte …
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The metabolism of iron-sulfur clusters during hydrogen peroxide stress in escherichia coli
… This enzyme belongs to a family of [4Fe-4S] dehydratases that are notoriously sensitive to univalent oxidation, and experiments confirmed that other members were also inactivated. In vitro and in vivo analyses showed that H2O2 directly oxidizes their solvent-exposed clusters in a Fenton-like …
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Design and synthesis of inhibitors of dTDP-D-glucose 4,6-dehydrate (Rm1B), and enzyme required for dTDP-L-rhamnose production in M. tuberculosis
… chosen as the primary focus: dTDP-D-glucose-4,6- dehydratase (RmlB). There are 3 steps in the reaction mechanism of RmlB: oxidation of the C4 position of dTDP-D-glucose to form a 4-keto structure, dehydration of the C6 position via the elimination of water and a subsequent reduction to result in a …
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Study of the baiE Gene in Bile Acid 7α-Dehydroxylating Bacteria
… The baiE gene encodes a bile acid dehydratase activity in Eubacterium sp.V.P.I. 12708. Using the polymerase chain reaction assay we determined the presence or absence of baiE-like genes in five clostridial bile-acid 7α-dehydroxylating strains: Clostridium sp. TO-931, Clostridium sp. …
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