Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"degradome"”.
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Computational analysis of small RNAs and the RNA degradome with application to plant water stress
… A snapshot of an mRNA degradation profile (degradome) can be captured through a new high-throughput technique called Parallel Analysis of RNA Ends (PARE) by using next generation sequencing technologies. In this thesis we describe a new user friendly degradome analysis software tool called …
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A SYNTHETIC TMRNA PLATFORM FOR ELUCIDATION OF BACTERIAL PROTEOME REMODELING UNDER STRESS
… synthetic peptide tag sequences and study the “degradome”. To study translational remodeling under stress, we modified the native tmRNA with an 6x-HIS isolation tag with the specific purpose of stabilizing, isolating, and characterizing the degradome in Escherichia coli. Using our inducible …
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Phosphate homeostasis and posttranscriptional gene regulation during arbuscular mycorrhizal symbiosis in Medicago truncatula
… regulation in M. truncatula mycorrhizal roots. Degradome analysis revealed that 185 transcripts were cleaved by miRNAs, of which the majority encoded transcription factors and disease resistance genes, suggesting a tight control of transcriptional reprogramming and a downregulation of defence …
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Small RNAs in Tomato: from defence to development
… approach was based on small RNA sequencing and degradome data to characterize targets of these miRNAs, including the recently discovered tomato TAS5 locus. I also generated transgenic tomatoes constitutively expressing target mimic RNAs that sequester different miR482/2118 members. These tomato …
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Global transcriptional, post-transcriptional, and translational regulation during soybean seed and seedling development
… a transcriptome-wide experimental method called degradome sequencing was carried out to directly detect cleaved miRNA targets. In this study, degradome libraries were constructed from immature soybean cotyledons representing three stages of development and from seed coats of two stages. …
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Transcriptional and Post-Transcriptional Regulation of Nodule-Specific Gene Expression in Soybean
… hormone signaling elements; (ii) small RNA and degradome/Parallel Analysis of RNA Ends (PARE) libraries were generated to identify miRNAs and their cleavage products respectively in nodule tissues; (iii) miRNA qPCR quantification methods were optimized; and (iv) the effect of misexpression of …
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Development of Computational Tools and Resources for Cotton microRNA Analysis
… Cleaveland software package for analysis of degradome sequencing data. Although cotton genome is still not available, huge cotton ESTs could be a good data resource for identification of cotton miRNAs and their targets. To better utilize cotton ESTs for miRNA identification, we globally …
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Genomic and genetic resource for soybean disease resistance improvement
… pre-identified miRNAs were also predicted using degradome sequencing reads as the direct evidence of decayed mRNAs. Two prediction tools yielded two distinct nonoverlapping sets of targets. Our findings on miRNAs in G. latifolia add to our knowledge of small regulatory RNAs in legumes and may …