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Showing 1 to 3 of 3 for “"deep mutational scan"”.
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Modeling the effects of site-specific amino-acid preferences on protein evolution.
… two existing methods, phylogenetics and deep mutational scanning, to understand the effect of site-specific amino-acid constraint on protein evolution. These two methods, one computational and one experimental, have complementary strengths and weaknesses. Phylogenetics provides methods to …
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Probing PAX6-DNA interactions using high-throughput yeast one-hybrid assays and deep mutational scanning
… I used a combination of yeast one-hybrid and deep mutational scanning in competitive growth assays to probe the functional consequences of almost all the possible single amino acid variants in the paired domain of PAX6. The results showcase the capabilities of the assay to reproducibly …
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Conformational engineering of human chemokine receptors and HIV-1 Env using deep mutational scanning
… However, by combining directed evolution and deep sequencing with site- saturation mutagenesis (SSM) to create unbiased, diverse libraries, it is now possible to track the phenotypic fitness of thousands of sequence variants in a single experiment. This is known as deep mutational scanning, …