Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"decapping"”.
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The Role of Edc3 in Drosophila Melanogaster Neuronal Development
… mRNA-decay 3 (Edc3) is a component of multiple decapping complexes that regulates the transcriptome by targeting a subset of transcripts for decapping for 5’-to-3’ messenger RNA (mRNA) decay. A missense mutation in the conserved Lsm domain of Edc3 has been reported in two siblings presenting …
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Analysis of the role of poly(A) -binding protein (PAB1) in the mRNA degradation process in yeast
… RRM3 domain of PAB1 inhibits deadenylation and decapping. mRNP structures involving the terminal PAB1 bound to poly (A) are also affected by RRM3 and control the end to deadenylation and apparently the commencement of decapping. These results indicate that PAB1 integrates and controls the …
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Identification of interacting partners of mammalian target of rapamycin complex 1 (mTORC1) assembly in human lymphocytes
… protein specific antibodies.Enhancer of mRNA decapping protein 4 (Edc4) was consistently identified as a new interacting protein with mTORC1 in both the endogenous and myc-tag raptor component of mTORC1. Edc4 has a suggested role in mRNA decapping and repression of miRNA mediated translation. …
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Functions of Dcp2 and Ski7 In Mrna Degradation
… is degraded by two alternative pathways. The decapping enzyme Dcp2 removes the 5` <sup>m7</sup>G cap of mRNAs, allowing Xrn1 to degrade the mRNA from the 5` end. Alternatively, mRNA is degraded from the 3` end by the RNA exosome.</p> <p>While decapping by Dcp2 is a critical step in mRNA decay, …
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Preclinical evaluation of a novel telomerase inhibitor in malignant cells of the hematopoietic system
… induced a time-dependent individual telomere decapping which was associated with loss of TRF2 and increased phosphorylation of p53. Ectopic expression of hTERT revealed the close correlation of the protective role of the catalytic activity, telomere length and cellular stability. We conclude …
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Functional analysis of the CAF1 protein
… synthetically lethal with defects in DHH1, a decapping regulator that is involved in controlling translation, and with certain defects in poly(A) binding protein (PAB1) that display decreased rates of translation and deadenylation. A caf1 deletion also had a more significant effect on …
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Transcriptional elongation defects enhance upstream poly (A) site utilization and SPT5 affects mRNA degradation through its physical and functional interaction with CCR4 -NOT complex
… the rate of deadenylation, but not that of decapping or 5'-3' degradation of mRNA. Re-examination of SPT5 location in the yeast cell confirmed that SPT5 is primarily nuclear but some SPT5 was also found to locate to the cytoplasm. These results support a role for SPT5/SPT4 in mRNA …
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The identification and characterization of the interaction between Upf1 and PAB1 during nonsense-mediated decay & the identification of novel protein complexes associated with translation termination factor eRF1
… deadenylation, but is not required for decapping or general decay during NMD. These results also revealed that this interaction plays a role in the normal shift in deadenylation mode for non-PTC containing mRNAs from the relatively slow mode of distributive deadenylation to that of rapid …
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Mechanism and Biological Scope of Target-Directed MicroRNA Degradation
… to bound targets to promote their deadenylation, decapping, and subsequent decay. Although knowledge surrounding miRNA biogenesis and effector functions is extensive, the understanding of how miRNAs decay is still relatively nascent. Recent global measurements of miRNA stabilities in cells of mice …
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Functions of The Trna Splicing Endonuclease and Other Adventures In Rna Processing
… “nibble” downstream of endonuclease cleavage and decapping but that its main function is in processing the 25S’ to the 25S rRNA in the cytoplasm. Trl1 can also act downstream of endonuclease decay in the TED pathway by phosphorylating the 5’ end of TSEN cleavage products. Though the other …
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Characterisation of the multi-isomeric protein nesprin-1 in p-body and mRNA dynamics
… p50Nesp1 was found to complex with mRNA decapping factors and translational repressers.<br/>More importantly, p50Nesp1 associated strongly with microtubules, both in vitro and in vivo, and was required for scaffolding P-body complexes to microtubules. By disrupting P-body-microtubule …
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Characterization of the instability elements present within the 3' UTR of the glutaminase mRNA in WKPT kidney cells
… may be mediated by the exosome. An in vitro decapping assay demonstrated that both the 5' → 3' and 3' → 5' degradation pathways are involved in the turnover of the GA mRNA. Determination of whether 5' → 3' or 3' → 5' decay pathway predominates in vivo will require further analysis. Antibodies …
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SYK promotes TGF-beta-induced P-body clearance in breast cancer cells through the enhancement of autophagy
… with the induction of EMT. Also, when mRNA-decapping enzyme 1A, DCP1A, is over-expressed, which results in the formation of aberrant P-bodies, EMT is blocked. In my report, I show that SYK expression and inhibition of mechanistic target of rapamycin, mTOR, by rapamycin both promote P-body …
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Relationship between Processing Body Formation, Epithelial-to-Mesenchymal Transition, and Invasion in Lung Adenocarcinoma
<p>Lung cancer is the leading cause of cancer-related deaths in the United States, largely due to its ability to metastasize. Epithelial-to-mesenchymal transition (EMT) is a process that enhances the ability of cells to lose their cell-cell contacts, invade, and enter the blood stream which are …