Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"deacylation"”.
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The biological properties of three trichothecene mycotoxins produces by fusaris
… toxicity was investigated by determining the deacylation rate of T-2 toxin to HT-2 toxin, one of the first steps in the detoxification of this trichothecene. The high deacylation rate catalysed by rat microsomes correlated with the low sensitivity of this species to T-2 toxin, whereas the low …
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Halo enol lactone and protio enol lactone inhibitors of alpha-chymotrypsin: Mechanism and stereospecific behavior of inhibition
… ketone group (transient inhibition). Deacylation of the second acyl enzyme leads to reactivation to free enzyme, which will be available for inactivation again. From the rate of the time-dependent irreversible inactivation and the rate of deacylation, a partition ratio between …
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Characterization of Leucyl -Trna Synthetase From Homo Sapiens and Escherichia Coli in Aminoacylation, Amino Acid Editing and Interdomain Interactions
… in the amino acid binding pocket failed to block deacylation of tRNA, indicating that the architecture of the amino acid binding pocket is different compared to other characterized LeuRSs. In addition, mutation of the universally conserved aspartic acid abolished tRNALeu deacylation. Surprisingly …
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Biochemical Analysis of the Molecular Factors of Leucyl-Trna Synthetase That Ensure Aminoacylation Fidelity
… the first class of mutants have maintained deacylation activity of mischarged tRNA as well as a robust leucylation activity. Therefore, the hypothesis is that this class of mutants charge tRNALeu, but fail to translocate these products to a competent amino acid editing site where they can be …
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Theoretical and experimental studies of slowly deacylating alpha-chymotrypsin acyl enzymes
The differential deacylation rates for alpha-chymotrypsin beta-phenyl acyl enzymes 13 and 14 have been studied using the techniques of molecular mechanics and molecular dynamics. These studies indicate that the ketone side-chain of slowly deacylating acyl enzyme 13 is hydrogen bonded to the NH of …
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Characterization of leucyl-TRNA synthetase from homo sapiens and escherichia coli in aminoacylation, amino acid editing and interdomain interactions
… in the amino acid binding pocket failed to block deacylation of tRNA, indicating that the architecture of the amino acid binding pocket is different compared to other characterized LeuRSs. In addition, mutation of the universally conserved aspartic acid abolished tRNALeu deacylation. Surprisingly …
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The Role of Gastrointestinal Microflora in the Metabolism and Toxicity of Trichothecene Mycotoxins
… to produce deepoxy metabolites, yielding only deacylation products. Rat cecal microflora efficiently biotransformed T-2, DAS and the polar trichothecenes, tetraol and scirpentriol, to their deepoxy metabolites demonstrating that lipid solubility is not a major factor in epoxide reduction.
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Peroxisome Proliferator -Activated Receptor (Ppar)alpha Regulation of Highly Unsaturated Fatty Acid Synthesis
… may shift the equilibrium of phospholipid deacylation/reacylation cycle toward accumulation of lysoPL. To test the hypothesis, first we used computer simulation to estimate affinity between lysoPL and PPARalpha. The model predicted lysoPL binding with high affinity. Next, binding affinity …
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Studies on the chemistry of taxol
… a tubulin assay was determined. A study of the deacylation and reacylation reactions of baccatin 111 was carried out in order to find conditions suitable for the preparation of 2-debenzoylbaccatin Ill , and thus 2-debenzoyltaxol. Finally, the reactions of taxol with various electrophilic …
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Studies of some reactions between electrophilic halogens and various conjugated systems in which intermediates or products having rearranged double-bond systems are concerned
… likely to be involved in processes of chloro-deacylation and of chloro-demethylation are discussed; a consideration of certain structural features of the substrates studied led to the conclusion that chloro-deacylation and chloro-demethylation were essentially processes initiated by ortho- …
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Solid-State 17O NMR as a New Probe to Study Acyl-Enzyme Intermediates
… intermediates were found to undergo hydrolysis (deacylation) with the following rate constants: DAB-CHT, 6.4 ± 0.1 × 10-6; oMC-CHT, 2.8 ± 0.1 × 10-4 s-1; pMC-CHT, 8.5 ± 0.3 × 10-3 s-1. Analysis of solid-state 17O NMR spectra obtained under the magic angle spinning (MAS) condition at 21 T for the …
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Constitutive Overexpression of Acyloxyacyl Hydrolase in Mus Musculus
… the LPS signaling pathway. Thus, AOAH deacylation of LPS may represent a mechanism by which animals control responses to Gram-negative bacteria. Prior to the experiments described in this study, mice deficient in AOAH were found to be susceptible to the long-term effects of LPS. Aoah-/- …
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Identification and Biochemical Characterization of Ghrelin O-Acyltransferase (GOAT)
… CoA is necessary in the assays to prevent the deacylation of [3H]octanoyl CoA by crude membrane preparations. Maximal GOAT activity is observed at pH 7.0, and there is no apparent requirement for metals as determined by a lack of inhibition by 1 mM EDTA. The apparent Km for proghrelin is 6 …
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ΜΕΛΕΤΗ ΠΑΡΑΜΕΤΡΩΝ ΕΓΚΕΦΑΛΟΝΩΤΙΑΙΟΥ ΥΓΡΟΥ ΣΕ ΘΕΡΑΠΕΙΑ ΠΕΙΡΑΜΑΤΙΚΗΣ ΜΗΝΙΓΓΙΤΙΔΑΣ ΑΠΟ HAEMOPHILUS INFLUENZAE TYPE B
… DID NOT DECREASE CSF INFLAMMATION, WHEREAS DEACYLATION OF THE LIPID A MOIETY OF LPS WITH NEUTROPHIL ACYL-OXYACYL HYDROLASE PRODUCED A SUBSTANTIAL REDUCTION IN MENINGEAL INFLAMMATION. IN THE SECOND PART OF THE STUDY THE EFFECT OF TREATMENT IN MENINGITIS WAS EVALUATED. THE BRAIN WATER CONTENT …
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Chemical studies of the C-4 position of baccatin III and taxol
… other acyl groups. Preliminary studies of the deacylation and reacylation of baccatin III were carried out in order to find conditions necessary for the preparation of 4-deacetylbaccatin III, and hence 4-deacetyltaxol. 4-Deacetyltaxol has now been prepared from baccatin III via two synthetic …
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Characterization And Identification Of Novel Regulators Of The Synthesis Of Phospholipids
… <p>Transcriptional regulation of Cld1-mediated deacylation of CL influences energy metabolism by modulating the relative contribution of glycolysis and respiration to ATP production. Interestingly, <em>CLD1</em> expression is responsible for defective growth and respiration in tafazzin-deficient …
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Identification of Acyloxyacyl Hydrolase, a Lipopolysaccharide Detoxifying Enzyme, in the Murine Urinary Tract
… lipopolysaccharides (LPS). Such limited deacylation has been shown to attenuate cytokine and chemokine responses to LPS, suggesting a role for AOAH in modulating (downregulating) inflammatory responses to invading Gramnegative bacteria. Prior to the experiments described in this report, …
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Enzymology of DHHC-mediated Protein S-Acylation
… is reversible. Consequently, cells use acylation/deacylation cycles to regulate protein localization, stability, and activity. A family of integral membrane enzymes called DHHC proteins because of a conserved Asp-His-His-Cys motif, catalyze protein <italic>S</italic>-acylation within cells. DHHC …
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Acyloxyacyl Hydrolase: Studies on Its Regulation and Function in Mus Musculus
… LPS, and degrade bacterial proteins. Enzymatic deacylation of LPS is thus an intrinsic, regulated mechanism by which DCs may modulate host responses to this potent bacterial agonist. To study the biological functions of AOAH, AOAH-deficient mice were generated by targeted gene disruption. AOAH …
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Regioselective Synthesis of Cellulose Esters
Cellulose is an extraordinarily abundant polymer that can be harvested and purified from trees and other renewable sources. Cellulose derivatives have been widely used as coatings, optical films, fibers, molded objects, and matrices for controlled release. The properties of cellulose derivatives …