Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 46 for “"de-novo-synthesis"”.
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De novo synthesis of high purity CD3 epsilon peptides utilizing SUMO expression system in bacteria
Cancer is one of the leading causes of death in the United States. Monoclonal antibody drugs became one of the commercially and clinically successful drugs for many diseases. Among the monoclonal antibodies, T cell-dependent bispecific antibodies directly target tumor cells for cancer treatment, …
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Role of Xenopus p21-activated kinase 2 (X-PAK2) in polar body formation during oocyte maturation
… prophase until shortly before ovulation. Under the influence of gonadotropic hormones, these oocytes resume meiosis and progress to metaphase II, in which they await fertilization. The transition from prophase to metaphase II is termed oocyte maturation. During oocyte maturation, no mRNA …
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Anticancer Effects of Vitamin E Forms and Their Long-chain Metabolites via Modulation of Sphingolipid Metabolism
Cancer is one of the leading causes of death. Studies have shown that vitamin E forms including gamma-tocopherol (γT), delta-tocopherol (δT), and gamma-tocotrienol (γTE) exhibited potent anticancer activities in various types of cancer cells. But molecular mechanisms underlying anticancer actions …
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Photoelectrochemical array platform for genomic scale DNA synthesis
… and synthetic biologists have increasing demand for large, high-fidelity constructs of synthetic DNA. Recent developments in harvesting oligonucleotides from DNA microarrays has proven that these can be assembled into large constructs of DNA for costs significantly less than traditional …
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High Light and Low Temperature Effect on D1 Synthesis and Turnover in the Chilling Sensitive Plant Lycopersicun Esculentum
… these combined stresses were found to cause a decrease in de novo synthesis of D1, a component of photosystem II (PSII) reaction center which is known to be damaged by high light. The decrease in D1 synthesis was not due to changes in the steady state level of psbA, D1 message. An increase in …
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DNA analysis of Ornithine Transcarbamylase (OTC) deficiency in South African patients
… a significant number are due to defects in enzymes of the urea cycle. This cycle has evolved to cope with waste nitrogen disposal and the de novo synthesis of arginine. There are five distinct enzymatic steps in the urea cycle, and defects in each, result in a biochemically …
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Reductive Carboxylation Is a Novel Pathway of Glutamine Metabolism That Supports the Growth of Tumor Cells with Metabolic Defects
… glucose and glutamine in the mitochondria provide precursors needed for de novo synthesis of proteins, nucleic acids and lipids. Yet, a subset tumors harbor genetic mutations in the electron transport chain or tricarboxylic acid cycle that disable normal oxidative mitochondrial function. …
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Role of the Haem Oxygenase Pathway in Protection against Vascular Dysfunction
… to be a major contributor to the ischaemic episode which can occur either by progression of a disease state (atherosclerosis or angina pectoris) or after surgical intervention (organ transplantation). As a new approach for the preservation of vascular function after ischaemia, one line of …
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Microfluidic gene synthesis
The ability to synthesize custom de novo DNA constructs rapidly, accurately, and inexpensively is highly desired by researchers, as synthetic genes and longer DNA constructs are enabling to numerous powerful applications in both traditional molecular biology and the emerging field of synthetic …
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The Effect of High Salt Intake on Betaine-Homocysteine S-Methyltransferase Expression in the Guinea Pig
… to the hypothesis that BHMT is down-regulated under conditions requiring the accumulation of betaine, such as high salt intake. BHMT is expressed in the livers of mammals and only a few species such as pig, guinea pig, and primates (including humans) have been shown to express BHMT in the kidney. …
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Synthesis and activity of tyrosinase in mouse skin melanocytes
… (E.C. 1.14.18.1) is a key enzyme in the biosynthesis of melanin. The control of melanin sythesis was explored in skin melanocytes of the following strains; wild type (C57BL/6J-C/C) (which maximally synthesize melanin at normal mammalian body temperature, Himalayan (C57BL/6J-cʰ/cʰ) (which …
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Sphingolipid synthases of Toxoplasma gondii and other organisms
… to emerging drug resistance and the possible side effects of the existing therapeutics there is a necessity to explore new drug targets. Sphingolipids are essential components of eukaryotic cell membranes and signal transduction pathways. Mammals produce sphingomyelin (SM) via a SM synthase, …
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Antioxidant responses of pea (Pisum sativum L.) protoplasts
… plant level were used. Sulfite and the superoxide generating herbicide, paraquat, were used as the oxidants. Final sulfite concentrations during photosynthetic incubations ranged from 1.5 mM to 30.0 mM. During the polarographic estimation of photosynthesis, CO₂-dependent O₂ evolution did not …
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Inhibition of Cell Proliferation through Regulated Intramembrane Proteolysis of CREB3L1
… the non-permissive parental Huh7 cells, we identified CREB3L1 as a host factor that inhibits proliferation of virus-infected cells. Upon infection with diverse DNA and RNA viruses, including murine gamma-herpesvirus 68, HCV, West Nile virus (WNV), and Sendai virus, CREB3L1 was proteolytically …
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Use of immunological procedures to measure rate of accumulation and degradation of inducible NADP-specific glutamate dehydrogenase during cell cycle of synchronous Chlorella
A new five-step purification procedure was developed which could be completed in five days with an 80 to 85 percent yield of electrophoretically pure nicotinamide adenine dinucleotide phosphate-specific glutamate dehydrogenase from Chlorella sorokiniana. A monospecific, highly purified antibody …
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Alterations in Mammary Gland Synthesis and Secretion of Fatty Acids in Response to Trans Isomers of Octadecenoic Acid or Conjugated Linoleic Acid Isomers
… (9/11CLA) or trans10,cis12-18:2 (10/12CLA) on de novo synthesis and desaturation of milk fatty acids in lactating cows fed unsaturated oils. In the first study, rumen fermenters were fed (50 g DM/d) orchardgrass or red clover with 0, 8, or 16 g/d corn replacing equal portions of forage DM. …
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Vaccinia Virus Binding and Infection of Primary Human Leukocytes
… being evaluated as a vector for vaccine development and cancer cell-targeting therapy. Despite the importance of studying poxvirus effects on the human immune system, reports of the direct interactions between poxviruses and primary human leukocytes (PHLs) are limited. We studied the …
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Metabolism of Biopterin and its Derivatives in Man and the Rat
In-vitro studies of tetrahydrobiopterin synthesis and dihydropteridine reductase activity have shown that these are affected by pharmacological preparations, phenylalanine and tryptophan metabolites, neurotransmitters and metal ions. Tetrahydrobiopterin given orally to man does not appear in the …
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Uncovering the Role of 5'-Nucleotidases in Pyrimidine Metabolism in Trypanosoma brucei
… the substantial animal reservoir. A better understanding of parasite metabolism is necessary to address these issues in treatment and vector control. T. brucei generates nucleic acids and nucleotide sugars through two pathways: pyrimidine salvage or de novo synthesis using glutamine. However, …
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