Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"de novo methylation"”.
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Induction and characterization of de novo methylation by benzo[A]pyrene in the cancer cell lines MCF-7 and MDA-MB-231
<p>The experiments presented were designed to test the hypothesis that the well-known carcinogen, benzo[a]pyrene has epigenetic effects, specifically the ability to alter cytosine methylation patterns. MCF-7 and MDA-MB-231 human breast cancer cells were treated for a period of sixty days with 100 …
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Inhibition of IFN-[gamma] promoter function by site-specific methylation
… to polarize into either THI cells or TH2 cells. Development of naive CD4+ T cells into TH1 cells is characterized by the expression of IFN-y and the silencing of IL-4, while development into TH2 cells is characterized by expression of IL-4 and silencing of IFN-y. Naive helper T cells are …
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An investigation of the contribution of cigarette smoking and human papillomavirus infection to the epigenetic modulation of cellular genes in cervical epithelium
… and human papillomavirus infection, two independent risk factors for cervical neoplasia, to the epigenetic modulation of cellular genes in cervical epithelium. To determine the temporal relationship between cigarette smoking and the detection of CDKN2A methylation in cervical cytological …
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Molecular Characterization of Mammalian De Novo DNA Methyltransferase Chromatin Recruitment
<p>Precise deposition of CpG methylation is critical for mammalian development and tissue homeostasis and is often dysregulated in human diseases. The genomic localization of de novo DNA methyltransferases 3A (DNMT3A) and 3B (DNMT3B) is facilitated in part by chromatin "reader" domains to establish …
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Oct4 and Sox2 regulate methylation at the Igf2/H19 imprinting control region
Mono-allelic expression of H19 and Igf2 depends on parental-specific methylation of an imprinting control region (ICR). Although CTCF sites maintain the absence of DNA methylation on maternal ICRs after fertilization, some Beckwith-Wiedemann patients showed maternal ICR methylation that correlated …
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Contribution of Lsh to DNA methylation reprogramming in embryonic stem cell, epiblast stem cell and embryoid body model systems
DNA methylation is a key epigenetic mark which undergoes global reprogramming during early mammalian embryonic development, resulting in almost complete erasure of the mark after fertilisation of the zygote. Genome-wide patterns of DNA methylation are subsequently re-established in the implanting …
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Regulation of Active DNA Demethylation and its Role in Fertility in Arabidopsis thaliana
… must maintain control over the balance between de novo methylation, maintenance of existing methylation, and active demethylation as it utilizes these processes to facilitate gene expression changes throughout the lifetime of the plant. Expression of the DNA demethylase REPRESSOR OF SILENCING1 …
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Identification of Factors Involved In Dna Methylation of Cpg-Island-Promoters
… cancer is mediated by aberrantly increased DNA methylation levels at promoter CpG islands (CGI). About one-fourth of empirically defined human promoters are surrounded by or contain clustered repetitive elements. It was previously observed that a sharp transition of methylation occurs between …
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The Regulation of Dna Methylation In Mammalian Development and Cancer
<p>DNA methylation is an essential epigenetic modification in mammals, as it plays important regulatory roles in multiple biological processes, such as gene transcription, maintenance of chromosomal structure and genomic stability, genomic imprinting, retrotransposon silencing, and X-chromosome …
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Regulation of De Novo and Maintenance Dna Methylation By Dnmt3A and Dnmt3B
<p>DNA methylation (5-methylcytosine, 5mC) is essential for the regulation of gene expression and integrity of the mammalian genome. It occurs predominantly in the context of CpG dinucleotides to form a symmetrical pattern on both DNA strands, which allows DNA methylation patterns to be …
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Analysis of prognostic and drug resistance markers in lung cancer
… rates to cisplatin-based chemotherapy are modest at best. In the case of SCLC (20% of cases) the initial response rates to cisplatin-based chemotherapy are high. However relapse, often with chemotherapy resistant disease, is all too common. There is a significant body of evidence that …