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Showing 1 to 20 of 34 for “"ddPCR"”.

  1. The Effects of Known PCR Inhibitors on Droplet Digital PCR Performance

    Kolar, William Patrick2015

    … inhibitors in samples. Droplet digital PCR (ddPCR) relies on absolute quantification of positive and negative droplets and therefore may be less susceptible to inhibition than traditional qPCR techniques. Samples were prepared with a range of known inhibitor concentrations including aluminum …

    tdl Repository record

  2. Cell-free fetal DNA (cffDNA) enrichment for non-invasive prenatal testing (NIPT): a comparison of molecular techniques

    Sillence, Kelly2016

    … (cfDNA). The sensitivity of droplet digital PCR (ddPCR) against real-time PCR (qPCR) was compared for fetal sex and RHD genotyping. In addition RHD zygosity testing was carried out for non-maternal samples. Consequently, Pippin Prep™ gel electrophoresis was combined with ddPCR analysis for the …

    plymouth Repository record

  3. Droplet digital PCR detection and next-generation sequencing of HIV-1 in breastmilk of breastfeeding women on antiretroviral treatment

    Hurree, Jennah NivashniDepartment of Pathology2025

    … in breastmilk using droplet digital PCR (ddPCR) and sequencing of viral variants in breastmilk using Illumina MiSeq next generation sequencing to inform transmission risk and efficacy of maternal ART clinical trials. HIV-negative breastmilk samples were spiked with 8E5 cells (a cell line …

    cape-town Repository record

  4. Using Environmental DNA Surveys to Assess Green Sea Turtle (Chelonia mydas) Presence in Mississippi and Louisiana

    Humphreys, Emma M2027

    … for use on the Droplet Digital<sup>™</sup> PCR (ddPCR<sup>™</sup>) platform. The modified ddPCR<sup>™</sup> assay was validated <em>in-vitro</em> with genomic DNA and <em>in-situ</em> via a tank experiment with a <em>C. mydas</em> individual. Locations of <em>C. mydas</em> strandings were also …

    usm Repository record

  5. Bartonella in Small Mammals from Montana and Idaho

    Greenman, Emily2030

    … approximately 37% based on droplet digital PCR (ddPCR) screening. Following ddPCR screening, individuals that yielded positive results were selected for further analysis including genetic sequencing of the RNA polymerase b-subunit (<em>rpoB</em>) gene. While 116 individuals of 313 tested …

    nmu Repository record

  6. Identifizierung und Charakterisierung von Genen für die Entwicklung der Nieren und des Urogenitalsystems

    Leimeister, CorneliaUniversität Würzburg

    … Mesenchym reguliert werden. Mit Hilfe der ddPCR und Transfilter-Organkulturen wurde die Genexpression von induziertem versus nicht-induziertem Mesenchym aus Mäuse-Nierenanlagen untersucht. Einzelne Kandidaten wurden auf differenzielle Expression durch Northern Blot Analyse überprüft und für …

    wurz-thes Repository record

  7. Identificazione di nuovi biomarcatori epigenetici per la diagnosi precoce del carcinoma colorettale

    MANNINO, MAURIZIOUniversità degli studi di Catania2021

    … ad alta sensibilità della droplet digital PCR (ddPCR). Dopo le analisi ddPCR, il potenziale diagnostico di quattro miRNA selezionati, hsa-miR-21-5p, hsa-miR-497-5p, hsa-miR-503-5p e hsa-miR-375 è stato valutato attraverso analisi statistiche e ulteriori valutazioni bioinformatiche . Le analisi …

    catania Repository record

  8. Advancing detection, genomics, and secretome analysis of oomycete pathogens Phytophthora capsici and Phytopythium vexans to explore mechanisms of pathogenesis and biocontrol solutions

    Villanueva OscarBrock University2025

    … amplitude-based multiplex droplet digital PCR (ddPCR) assay. Designed for simultaneous quantification of P. capsici, Trichoderma asperellum, and Trichoderma gamsii in soil, this assay was validated in field trials and offers high sensitivity and specificity. The assay displayed a high …

    brock Repository record

  9. Molecular Pathology: Potential Biomarkers For The Detection Of Down Syndrome Pregnancies

    Garout, Raed2022

    … We will also use multiplex droplet digital PCR (ddPCR) using cell-free fetal DNA (cffDNA) to target different genes on chromosomes 21, 18, and 13 to screen for trisomy 21 (T21), trisomy 18 (T18) and trisomy 13 (T13), respectively. PRSS56 and SPINK7 proteins are secreted proteins and found to be …

    plymouth Repository record

  10. Transmission of tuberculosis in high school students in Worcester, South Africa

    Bunyasi, ErickDepartment of Pathology2020

    … with high ventilation risk (>1,000 ppm) and ddPCR air sampling for Mycobacterium tuberculosis DNA, with active TB symptom screening among learners. Results 1) There is paucity of data on adolescent LTBI and TB disease prevalence in high TB burden African countries (1990–2018). Based on the …

    cape-town Repository record

  11. Identificazione e Validazione di Hotspot di Metilazione del DNA come Biomarcatori per il Melanoma Cutaneo

    FALZONE, LUCAUniversità degli studi di Catania2021

    … were evaluated by using droplet digital PCR (ddPCR) while the methylation levels of the DNA methylation hotspots computationally identified were assessed using a custom protocol developed by the Laboratory of Experimental Oncology of the University of Catania defined Methylation Sensitive …

    catania Repository record

  12. microRNAs in Uveal Melanoma: in vivo findings and computational analysis

    FALLICO, MATTEO ROBERTOUniversità degli studi di Catania2020

    … miRNAs under investigation. Droplet digital PCR (ddPCR) was used for both liquid biopsy and tissue samples. A novel computational analysis for the three selected miRNAs was performed in order to corroborate the experimental results. Results: Significantly higher expression levels of miRNAs …

    catania Repository record

  13. The microbiology of chronic suppurative otitis media

    Neeff, MichelResearchSpace@Auckland2023

    … relevant bacteria. Droplet digital PCR (ddPCR), amplicon sequencing and multiplex PCR were utilised in this study. The distribution of the bacteria within the tissue was examined using histology and Gram staining. Genera Staphylococcus and Pseudomonas, the main pathogens reported in …

    auckland-ms Repository record

  14. Circulating tumour DNA: A non-invasive biomarker for melanoma

    McEvoy, Ashleigh CavellEdith Cowan University, Research Online, Perth, Western Australia2018

    … To do this we developed a new and innovative ddPCR TERT mutation assay and investigated its sensitivity alongside current assays in detecting mutations in melanoma tissue containing a small fraction of tumour cells. The significance of ctDNA for patient monitoring relative to current methods …

    edithcowan Repository record

  15. Impact of Wastewater Effluent on Surface Water Resistomes in a Small Lower-Income Community with Deteriorated Sewage Infrastructure

    Maldonado Rivera, Gabriel EnriqueVirginia Tech2025

    … droplet digital polymerase chain reaction (ddPCR), and DNA-sequencing. A significant difference was observed in the relative abundance of total ARGs between upstream and effluent samples (p =1.9x10^(-5) ), whereas no significant differences were observed between effluent and downstream …

    vt Repository record

  16. Bluttest statt Mammografie-Sreening?

    Krüger, SandraChristian-Albrechts-Universität zu Kiel

    … sowie methylierungsspezifische Analysen mittels ddPCR und Pyrosequenzierung. Die statistische Auswertung umfasste ROC-Analysen, Sensitivitäts- und Spezifitätsberechnungen sowie Subgruppenanalysen zwischen benignen Läsionen, DCIS und invasiven Mammakarzinomen. Die Ergebnisse zeigen, dass bestimmte …

    cau-kiel Repository record

  17. Development and evaluation of methodologies for analysis of CTC and ctDNA in patients with ovarian carcinoma

    Asante, Du-BoisEdith Cowan University, Research Online, Perth, Western Australia2022

    … were validated using droplet digital PCR (ddPCR). For ctDNA, the frequencies of genes affected by non-synonymous somatic mutations included TP53 (67%), KRAS (11%), PIK3CA (2%) and SF3B1 (2%). A total of 41 gene variants were identified in either pre- or post-NACT samples; 36 (87.8%) of them …

    edithcowan Repository record

  18. Identificazione del pattern di espressione dei microRNA nei pazienti con cancro del colon-retto e sua modulazione favorevole dopo l'arricchimento del microbiota intestinale con i probiotici

    GATTUSO, GIUSEPPEUniversità degli studi di Catania2022

    … ad alta sensibilità Droplet Digital PCR (ddPCR) è stato possibile valutare la disregolazione dei livelli di espressione dei quattro miRNA selezionati sia in campioni di biopsia liquida che in campioni di tessuto paraffinato (FFPE) di tumore e mucosa normale adiacente ottenuti da pazienti …

    catania Repository record

  19. The Wandering Mussels: Analyzing the Ecological Impact of Dreissenid Mollusks Across Spatial and Temporal Scales in Eastern Ontario Using Environmental DNA

    Tian, Haolun

    … I tracked quantitative dynamics via qPCR and ddPCR on bloom forming cyanobacterial species and toxin producing genes using high-resolution sedimentary eDNA chronologies. Reinforced by spatial and temporal comparisons, these analyses revealed a correlation inversion between cyanobacterial …

    queens Repository record

  20. Investigating drivers of cyanobacterial blooms in Aotearoa – New Zealand lakes using sedimentary ancient DNA

    Picard, MaïlysThe University of Waikato2023

    … using metabarcoding and droplet digital PCR (ddPCR) in Chapter 2. Bloom-forming species were present in all lakes prior to human arrival; however their overall abundance was low. Total cyanobacteria abundance and richness increased in all lakes after European settlement but was very pronounced …

    waikato-masters Repository record

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