Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"daf-2"”.
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The Recycling GTPase, RAB-10, Regulates Autophagy Flux in <i>Caenorhabditis elegans</i>
… expression and blocked tissue remodeling in <em>daf-2/IIR</em> mutants. The goal of this screen was to identify new or previously characterized endocytic genes that function in autophagy, further elucidate the mechanism by which these genes control autophagy, and enhance our understanding as to …
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DNA Damage-dependent Regulation and Function of akt-1 in Caenorhabditis elegans
… the Insulin-like receptor tyrosine kinase (RTK) DAF-2, the class I PI3K AGE-1 and the 3-phosphoinositide-dependent protein kinase 1 (PDK1) homologue PDK-1. This pathway ultimately controls the action of Akt homologues on the forkhead transcription factor DAF-16. The C. elegans Akt orthologue …
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Role of BEC-1/Beclin 1 and Autophagy Genes in <i>C.elegans</i> Germline Cell Proliferation
… acts independently of the GLP-1/Notch or DAF-7/TFG-β pathways, but interacts with components of DAF-2/IIR signaling pathway, to potentiate germline proliferation during development. Moreover, BEC-1/BECN1 requires DAF-18/PTEN but not DAF-16/FOXO for this function and can both promote and …
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A Role for Insulin Signaling in Regulating the PTEN Tumour Suppressor in Caenorhabditis Elegans
… I used C. elegans as a model and showed that DAF-2, the worm homolog of IR, is a negative regulator of DAF-18, the worm homolog of PTEN. In addition, I showed that DAF-28, the worm homolog of insulin, also negatively regulates DAF-18/PTEN. I used western blot and immunostaining to show that …
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The Impact of Insulin signalling on a C. elegans Alzheimer’s Disease Model
… signalling (IIS) pathway via an insulin receptor daf-2(lf) mutation or expression of antagonistic insulins not only suppressed these adult phenotypes but also alleviated similar impairments observed in the earliest larval stage (L1), such as reduced starvation survival and motility. These results …
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Non-Canonical Axonal Insulin Receptor Signaling Drives Aversive Olfactory Learning
… isoform of the insulin receptor DAF-2 in aversive learning. I perform epistasis studies of ist-1 with other members of the insulin signaling pathway to ask how they regulate aversive learning together. In Chapter 4, I characterize neuronal mechanisms involved in odor detection and …
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Identifying the Role of DAP-1 and H3.3 Histones in Starvation and Longevity in <i>Caenorhabditis Elegans</i>
… We establish that the transcription factor DAF-16/FOXO which acts downstream of the Insulin-like/DAF-2 signalling pathway, also governs the expression of DAP-1 during starvation and thus facilitates organismal survival in response to starvation. Overall, we establish DAP-1 as important …
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Autophagy and Lipid Homeostasis in C. elegans
… development in C. elegans. Moreover, long-lived, daf-2/InR and glp-1/Notch loss of function mutants, characterized by increased lipid stores, also required autophagy to maintain neutral lipid accumulation. Reduced lipid levels could be due to a lack of lipid synthesis, defects in the storage, or …
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Einfluss von Drogenextrakten und Naturstoffen auf die endotheliale NO-Synthase
… eNOS-bedingten L-Citrullin-produktion und ein DAF-2 Fluoreszenzassay zur Messung der eNOS-bedingten NO-Produktion. Getestet wurden Extrakte und Naturstoffe, bei denen bereits positive kardiovaskuläre Eigenschaften wie Vasodilatation bekannt bzw. in der Diskussion waren. Keinen Einfluss auf die …
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Exploiting entomopathogenic nematode neurobiology to improve bioinsecticide formulations
… in S. carpocapsae may be heavily regulated by Sc-daf-2 and Sc-daf-7, which has been described in C. elegans previously. Host finding behaviour was enhanced in Breton strain, and transcriptome analysis revealed many GPCRs and neuropeptides to be upregulated in Breton, relative to the other two …
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Combining microbial functional metagenomics and Caenorhabditis elegans genetics to uncover and characterise novel bioactive compounds
Marine bacteria produce a wide array of biologically active metabolites involved in defence strategies and pathogenesis against a range of metazoans target organisms. In return bacteriovorous metazoans, including nematodes, have developed sophisticated strategies to neutralise or avoid such …