Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"dPCR"”.
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Evaluation of Digital PCR (dPCR) for the Quantification of Soil Nitrogen Turnover Bacteria in Wetland Mesocosms in Response to Season, Fertilization, and Plant Species Richness
… The chip-based QuantStudio 3D digital PCR (QS3D dPCR) system was used to quantify ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB), comammox, anammox, and denitrifiers. Principal component analysis (PCA) was used to identify dominant patterns in the microbial community and …
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Una nueva visión marítima para América Latina y el Caribe: hacia un desarrollo portuario sostenible. Análisis exploratorio del caso de la Delegación Portuaria de Coronel Rosales, a partir de la visión de los actores locales
… de la Delegación Portuaria de Coronel Rosales (DPCR), durante el año 2011, considerando la perspectiva de los actores locales, a partir de examinar la percepción que tienen de la situación actual y futura de la DPCR, y observando si están alineadas a las nuevas tendencias, con el fin de aportar …
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Characterisation of a public T cell clonotype enriched in m.tb infected individuals who do not progress to tuberculosis
… Methods: A custom-designed digital PCR (dpcr) assay was used to quantify the frequencies of total T cells and kiif.tb T cells from IGRA-, IGRA+, and TB patients. Identification and characterisation of kiif.tb T cells by flow cytometry was performed using a custom flow cytometry-compatible …
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Development of digital PCR probe assays targeting SNPs to monitor co-cultured cell lines
… a probe-based digital polymerase chain reaction (dPCR) setup with high sensitivity and specificity for monitoring various cell lines in a co-culture over time. The designed probes target cell line unique single nucleotide polymorphisms (SNPs) of each myxoid liposarcoma (MLS) and a fibrosarcoma …
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Rhizosphere/Soil Microorganisms in the Phytoremediation of Biphenyl and Dioxane
… Direct polymerase chain reaction (DPCR) without prior DNA extraction and DGGE yielded the same results as PCR assays using extracted DNA in the analysis of microbial populations in dioxane- and biphenyl-degrading consortia. Therefore, the combination of DPCR and DGGE has the …
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Investigating the role of circulating cell-free dna as a mechanistic biomarker in inflammatory bowel disease: development of an integrated precision-medicine enabled platform in Scotland
… digital polymerase chain reaction (dPCR) for COX3, ND2 and GAPDH genes, fragment analysis with the Agilent BioAnalyzer, and cfDNA sequencing using both Nanopore and Illumina platforms. RESULTS: Patients with highly active IBD (requiring admission to hospital) had significantly higher …
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Circulating Tumour DNA in Localised Urological Cancers
… mutations. Sequencing-based methods and dPCR were employed to analyse clinical samples including plasma and urine, to detect and quantify mutDNA. Molecular and clinical data were integrated to explore potential areas of application of mutDNA analysis. For bladder cancer, mutDNA was …
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Human impact on the McMurdo Dry Valley soils of Antarctica: Extending the limits of DNA detection
Antarctica is commonly regarded as the most pristine place on Earth and is safeguarded by the Antarctic Treaty System (ATS). The ATS designates the continent as a “natural reserve, devoted to peace and science” and sets out environmental principles regulating human activities. The obligations under …