Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"dNTPs"”.
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Metal-Organic Framework-Based Biosensors for Enzyme-Linked Assays
… compounds like deoxynucleotide triphosphates (dNTPs) and pyrophosphate (PPi). The findings demonstrate that Eu(BTC) and TbEu(BTC) MOFs can effectively differentiate between dNTPs and PPi in an isothermal amplification buffer (Tris buffer, pH = 8.8), due to their rigid crystalline structures …
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New insights into the mechanism of dna replication on unmodified and benzo[a]pyrene modified templates using surface plasmon resonance
… complex in the presence of incorrectly pairing dNTPs. We present and discuss the implications of a new model for DNA synthesis that involves a dNTP selection cycle. This selection cycle shows how polymerases aid in the removal of mispairing dNTPs from their active sites in an effort to actively …
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On-demand production of deoxynucleotides from genomic DNA
… namely the 2'-deoxynucleoside 5'-triphosphate (dNTPs) building blocks of DNA, in a manner that can be integrated into a NAAT workflow. A unique top-down approach to produce dNTP monomers from a genomic DNA polymer is investigated, contrary to traditional bottom-up synthesis methods, which rely …
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Discovery and investigation of the novel overall activity allosteric regulation of the Bacillus subtilis class Ib ribonucleotide reductase
… by ATP and 2'-deoxynucleoside 5'-triphosphates (dNTPs), but not overall activity regulation by ATP and dATP. However, the S. enterica enzyme is not a good general model for Ib RNRs because it is not essential under most growth conditions, including pathogenesis. Other bacteria pathogenic to …
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From the End to the Middle: Regulation of Telomere Length and Kinetochore Assembly by the RNR Inhibitor SML1
… and requires an adequate and balanced supply of dNTPs. In Saccharomyces cerevisiae, de novo dNTP synthesis through nucleotide reduction by the Ribonucleotide reductase (RNR) enzyme is the sole method of production. Hence, RNR activitity is highly regulated via allosteric control, transcriptional …
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Investigations of the inhibition mechanisms of human ribonucleotide reductase by gemcitabine-5'-diphosphate and saccharomyces cerevisiae ribonucleotide reductase by Sml1
… of nucleotides to deoxynucleotides supplying the dNTPs required for DNA replication and DNA repair. Class I RNRs require two subunits ([alpha] and [beta]) for activity. The [alpha] subunit binds the substrates and the allosteric effectors that govern specificity and turnover. The 32 subunit houses …
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Homologous Strand Exchange and DNA Helicase Activities in Plant Mitochondria
… this enzyme poorly utilizes any other NTPs or dNTPs. The enzyme requires Mg2+ for activity. This enzyme only has 3' to 5'unwinding activity. The optimal conditions for mitochondrial DNA helicase are 2 mM ATP, 8 to 10 mM Mg2+,100 to 200 mM NaCl and 37-42 oC incubation for one hour or longer …
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HIV needs the small molecule IP6 to build its capsid
… In addition, they are proposed to recruit dNTPs to facilitate encapsidated DNA synthesis. Here I show that infection and conical capsid formation of K25A can be rescued by compensating mutants without restoring the charged K25 ring. This suggests that K25 is necessary for assembly but not …
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Structural studies of allosteric regulation in the class Ia Ribonucleotide reductase from Escherichia coli
… activity regulation) of deoxyribonucleotides (dNTPs) in the cell. The molecular basis of this regulation has been enigmatic, largely due to a lack of structural information about how the [alpha]2 and [beta]2 subunits interact. Here, we present the structure of a complex between the [alpha]2 and …
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The development of PCR internal controls (PICs) for forensic DNA analysis
… reaction optimisation, involving the addition of dNTPs and MgCl2. The presence of PICs provided information on the amplification performance and post-PCR processing and can assist with the interpretation of human DNA profiles. The position of the fragments also allowed PICs to be used as sizing …
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Enzymatic Polymerization of High Molecular Weight DNA
… to incorporate a wide range of unnatural dNTPs into the growing chain, such as, hydrophobic fluorescent dNTP and fluoro modified dNTP. By harnessing the encoded nucleotide sequence of an initiator and the chemical diversity of monomers, TcEP enables us to introduce molecular recognition …
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Initial Stages of Hepatitis B Virus Replication
… polymerase assay with 32P-labeled ATP and cold dNTPs with isolated core particles. HBV DNA polymerase incorporates NTPs by F436 substitution to glycine which is smaller than valine. This result indicates that bulky amino acid in dNTP binding cleft acts as a steric gate in dNTP selections. Even …