Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"dNTP"”.
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Mutational studies in the dNTP binding pocket of human immunodeficiency virus type 1 reverse transcriptase
… of Q91 were found to be highly resistant to ddNTPs and exhibited greater stringency in discriminating between correct and incorrect nucleotides. These results suggest possible interaction of Q91 with other residues in the dNTP binding pocket that may be responsible for conferring greater …
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From the End to the Middle: Regulation of Telomere Length and Kinetochore Assembly by the RNR Inhibitor SML1
… and requires an adequate and balanced supply of dNTPs. In Saccharomyces cerevisiae, de novo dNTP synthesis through nucleotide reduction by the Ribonucleotide reductase (RNR) enzyme is the sole method of production. Hence, RNR activitity is highly regulated via allosteric control, transcriptional …
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New insights into the mechanism of dna replication on unmodified and benzo[a]pyrene modified templates using surface plasmon resonance
… are induced by the identity of the incoming dNTP, and its ability to effectively base pair with the templating base. The presence of the next correct dNTP is known to induce a conformational change of the polymerase and lead to an increase in KF-DNA binding. Conversely, the presence of an …
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The Impact of Nucleoside Sugar Modification on Biochemical DNA Transactions
… Polymerase discrimination operates at both dNTP insertion and subsequent elongation steps, and involves several energetic and structural factors that are as yet incompletely understood. While base pairing interactions have been studied extensively, substantially less is known about the role …
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Engineering T Cell Receptors to Examine the Role of Affinity and *Kinetics in T Helper Cell Function
… included: alteration of error-prone PCR dNTP concentrations, increasing induction media galactose concentrations, and generation of three-domain TCR constructs (Valpha-VbetaCbeta).
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Molecular systematics and population genetics of Hypoxylon truncatum sensu Miller using RAPD
… different concentrations of DNA, MgCl$\sb2,$ dNTP, gelatin, primer, and Taq polymerase, different temperature profiles, and presence of RNA. The result of this study suggest that concentrations of reaction components studied are highly inter-dependant in their effects, and that RNA can …
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Isolation of antimutator yeast strains: an exploration of DNA replication fidelity
… gene expression and up-regulates dNTP synthesis during the S-phase checkpoint. We demonstrated that deletion of Dun1 (dun1-delta) rescues cells from error-induced extinction caused by combined defects in Pol epsilon proofreading and base selectivity (pol2-4,M644G) or Pol epsilon …
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Enzymatic Polymerization of High Molecular Weight DNA
… of 2’-deoxyribonucleoside 5’-triphosphates (dNTP, monomer) from the 3’-hydroxyl group of an oligodeoxyribonucleotide (initiator). We termed this enzymatic synthesis method: TdT catalyzed enzymatic polymerization, or TcEP.</p><p>Specifically, this dissertation is structured to address three …
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Regulación de la rutas de tolerancia a daños replicativos dependientes de RAD6, RAD5 y RAD52 durante el ciclo celular en Saccharomyces cerevisiae
… especializadas capaces de incorporar un dNTP opuesto a la lesión y es propenso a errores, HR usa la cromatida hermana para reparar las lesiones de ssDNA y en su mayoría está libre de errores. En este trabajo se demuestra que las proteínas de HR Rad52, Rad51 y Rad57 colaboran con la …
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Initial Stages of Hepatitis B Virus Replication
… amino acid, phenylalanine 436, at supposedly dNTP binding cleft of HBV DNA polymerase was changed to smaller amino acids, glycine or valine. NTPs incorporation capacity of HBV DNA polymerase was tested by endogenous polymerase assay with 32P-labeled ATP and cold dNTPs with isolated core …
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IN VITRO AND IN VIVO ACTIVITY OF DINB: ROLE IN MUCOID CONVERSION OF PSEUDOMONAS AERUGINOSA
… as metal ion cofactors for catalysis. The Km for dNTP is over 600-fold lower when manganese is present compared with magnesium, indicating a role for the cofactor in changing the affinity of the enzyme for dNTP. Given the E. coli DinB’s preference for creating -1 frameshifts in poly-guanine runs, …
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Elucidating the mechanisms and dynamics of drug-mediated inhibition and latency reversal in HIV-1 (HIV-1)
… This is further supported by the efficiency of dNTP or NRTI incorporation in the presence of NNRTI with multiple DNA/DNA substrates, which are representative of different sites in the template sequence. We also show that there are site-dependent differences in the polymerase properties of RT, …
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Investigation Into The Binding Interactions Of Klenow Fragment To Dna Modified With Carcinogens Af And Aaf Using Surface Plasmon Resonance
… presence and absence of nucleotide. Incorrect dNTP showed the least KF destabilization binding to the dG-AF mismatched DNA constructs. It is possible that the bulky, mismatched primer template terminus rearranges the active site allowing the modified dG to rotate and serve as a template for the …
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Structural determinants of murine leukemia virus (MLV) reverse transcriptase (RT) important for fidelity and drug resistance in vivo
… V223 of the YVDD box, several residues of the dNTP-binding site, as well as residues residing in the RNase H domain of MLV RT. A number of these MLV RT mutants resulted in statistically significant decreases in fidelity (1.2 to 2.8-fold) whereas two mutants showed a statistically significant …
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Uncovering the Role of 5'-Nucleotidases in Pyrimidine Metabolism in Trypanosoma brucei
… C and U. Disruption of this cycle leads to dNTP imbalance and DNA damage, which signals the downregulation of glutamine uptake. Through the study of 5'-HDNT, I have unmasked a potential cell death pathway that goes beyond de novo and salvage pathways to implicate cellular metabolism in T. …
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On-demand production of deoxynucleotides from genomic DNA
… namely the 2'-deoxynucleoside 5'-triphosphate (dNTPs) building blocks of DNA, in a manner that can be integrated into a NAAT workflow. A unique top-down approach to produce dNTP monomers from a genomic DNA polymer is investigated, contrary to traditional bottom-up synthesis methods, which rely …
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Discovery and investigation of the novel overall activity allosteric regulation of the Bacillus subtilis class Ib ribonucleotide reductase
… by ATP and 2'-deoxynucleoside 5'-triphosphates (dNTPs), but not overall activity regulation by ATP and dATP. However, the S. enterica enzyme is not a good general model for Ib RNRs because it is not essential under most growth conditions, including pathogenesis. Other bacteria pathogenic to …
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Structural studies of allosteric regulation in the class Ia Ribonucleotide reductase from Escherichia coli
… activity regulation) of deoxyribonucleotides (dNTPs) in the cell. The molecular basis of this regulation has been enigmatic, largely due to a lack of structural information about how the [alpha]2 and [beta]2 subunits interact. Here, we present the structure of a complex between the [alpha]2 and …
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ATR SIGNALING IN GENOME REPLICATION AND MAINTENANCE
… cells encounter many challenges, such as dNTP depletion, transcription intermediates, chemical adducts, double strand breaks, and heterochromatin. Replication stress induced by these challenges activates the sensor kinase ATR in mammals (Mec1 in S. cerevisiae), which coordinates a …
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