Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"cytosolic fractions"”.
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Micro-analysis of adenylate cyclase and cyclic AMP-dependent protein kinase activities in human mammary tumors
… permits comparisons to be made with particle and cytosolic fractions prepared from mammary tumors. The potential for cAMP formation by adenylate cyclase and mediation of cAMP effects by cAMP-dependent protein kinase were studied to provide information on the levels of enzyme activity and the …
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Acute and chronic effects of nitric oxide on cardiomyocyte guanylyl cyclase : implications for the modulation of the heart following environmental, physiological and clinical exposure to nitric oxide
… septic condition. GC activity was determined in cytosolic fractions of the cardiomyocytes and also in 'intact' cardiomyocytes following permeabilization with digitonin. Cardiomyocytes were shown to possess NO-sensitive GC which was localized in the cytosol. GC activity in the absence of added NO …
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Identification of substrate-selective histone deacetylases and their inhibitors that mediate HER2 transcript stability
… a protein identified by mass spectrometry in cytosolic fractions containing rapidly degrading HER2 mRNA as a protein appearing hyper-acetylated within hours of breast cancer cell exposure to a pan-HDAC inhibitor. Studies conducted using pan-HDAC inhibitors like Trichostatin A (TSA) as well as …
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Interspecies Pharmacokinetic Scaling and Metabolism of Alcohols and Glycols
… glycol was also characterized in hepatic cytosolic fractions from rat, rabbit, dog and human and in-vitro in-vivo correlation for the hepatic disposition parameters was assessed. Finally, allometric scaling relationships for ethanol and propylene glycol PK parameters (in-vivo and in-vitro) …
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Towards the development of fluorescent probes targeting aldehyde dehydrogenase (ALDH) in cancer. Expression and epigenetic modulation of ALDH1A1, ALDH2 and ALDH3A1 in selected in vitro models.
… 70 ºC. Metabolism studies were conducted using cytosolic fractions from rat liver enriched in ALDHs, yeast ALDH and human recombinant ALDH1A1. Some evidence was demonstrated which linked ALDH metabolism with aldehyde functionalities of hydrolysed AAQs (HAAQs). The AAQs were shown to emit far-red …
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Towards the development of fluorescent probes targeting aldehyde dehydrogenase (ALDH) in cancer. Expression and epigenetic modulation of ALDH1A1, ALDH2 and ALDH3A1 in selected in vitro models.
… 70 ºC. Metabolism studies were conducted using cytosolic fractions from rat liver enriched in ALDHs, yeast ALDH and human recombinant ALDH1A1. Some evidence was demonstrated which linked ALDH metabolism with aldehyde functionalities of hydrolysed AAQs (HAAQs). The AAQs were shown to emit far-red …
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IL-2 and Antisense TGF-B1 Gene Therapy of the H238 Tumor and Analysis of TGF-B Receptors
… cell lysate was also resolved into membrane and cytosolic fractions, and these probed with for the type 1 or the type2 receptor. In comparision, to positive cell lysate controls for these receptors, H238 cell lysates showed a comparitively less amount of receptor expression. Receptor expression …
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Escape to the interior: Characterization of the cytosolic delivery of C-terminal cargo subdomains by the N-terminus of pasteurella multocida toxin and subsequent toxin-mediated downregulation of Gaq-PLCb1 signaling through depletion of Gaq from the plasma membrane
… shown that PMT-N facilitates host cell entry and cytosolic delivery of the C-terminal catalytic cargo from late endocytic vesicles. The putative cargo of PMT is a 78-kDa polypeptide of three discrete modular subdomains C1-C2-C3, for which there is a crystal structure available. Subdomain C1 …
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Studies on the mode of action of dermonecrotic toxins: Effects on adipogenic signaling pathways and delivery of c-terminal cargo into the cytosol
The dermonecrotic toxin family consists of the dermonecrotic toxin from Bordetella spp. (DNT), the cytotoxic necrotizing factors from Escherichia coli (CNF1, CNF2 and CNF3) and Yersinia pseudotuberculosis (CNFY), and the mitogenic toxin from Pasteurella multocida (PMT). These relatively large …