Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 27 for “"cytoplasmic dynein"”.
-
The structure of the cytoplasmic dynein tail
Cytoplasmic dynein is a molecular motor that moves cargos along microtubules. Dynein, together with its large co-factor dynactin, is responsible for the vast majority of traffic towards the centre of the cell. The largest subunit of the dynein complex is called the dynein heavy chain (DHC). The DHC …
-
Cargo Specific Regulation Of Cytoplasmic Dynein By Effector Proteins
… driven almost completely by the molecular motor, cytoplasmic dynein. Yet, it remains unclear how dynein is spatially and temporally regulated given the variety of cargo that must be properly localized to maintain cellular function. Previous work has suggested that adaptor proteins provide a …
-
Novel Regulatory Mechanisms of Cytoplasmic Dynein: A Role for the Complex Base
Cytoplasmic dynein is unique among cellular motors not only in its size and complexity but also its diversity of functions. It is essential for many mitotic and interphase transport processes and its misregulation or malfunction results in devastating neurological disorders. Over 20 years of …
-
An Examination Of The Cytoplasmic Dynein Stepping Mechanism At The Single Molecule Level
… mechanism of the cellular transport motor, cytoplasmic dynein. Cytoplasmic dynein walks along microtubules toward the minus end and is responsible for a wide range of cellular functions including cargo transport and chromosome alignment during cell division. This work employs a position and …
-
Role of Microtubule Motor Proteins in Adenoviral Infections
… the MT minus-end directed motor protein complex cytoplasmic dynein, which functions in organelle positioning, cell migration, cell division, and cell differentiation in uninfected cells. Cytoplasmic dynein subunits known to interact with physiological cargo also bind directly to the adenovirus …
-
Mechanisms and Regulation of Organelle Transport in Xenopus Melanophores
… melanosomes possessed the motor proteins cytoplasmic dynein and kinesin-II and were able to move along microtubules in vitro. These motors retained their regulated state following purification. Melanosomes were also found to possess the actin-based motor myosin-V and to move on actin …
-
Mutational and functional analyses of the dynein heavy chain indicate the existence of an apical-sistal transport cycle in cells
… by the microtubule based molecular motors cytoplasmic dynein and kinesin. Dynein carries out the inward transport of cargos whereas kinesins are responsible for the outward movement. Although intracellular transport is necessary for the function of all cells, polarized cells in particular …
-
Involvement of a specific ubiquitin ligase in the assembly of the dynein motor in the filamentous fungus Neurospora crassa
… The two microtubule based motors, kinesin and cytoplasmic dynein, work in concert to move cargoes outward and inward, respectively, in the cell. In polarized cells, molecular motors are of even greater importance due to a need for long distance transport and maintenance of proper polarity. …
-
Signals Regulating Organelle Transport in Melanophores
… its dephosphorylation. Immunoprecipitation of cytoplasmic dynein showed that proteins at the molecular weights of the light intermediate chains are differentially phosphorylated in aggregated and dispersed cells. Both motor subunits of kinesin II are phosphorylated in melanophores, but were not …
-
Messenger Ribonucleoprotein (Mrnp) Complexes: Proteomic and Functional Analysis
… both opposite-polarity motors, kinesin-1 and cytoplasmic dynein, are required for dFMRP-RNP transport, implying a coordination model rather than tug-of-war model for motor activities. Furthermore, while KLC is dispensable, dynactin is required for dFMRP-RNP transport, indicating a novel …
-
ANALYSIS OF TWO NOVEL COMPLEXES THAT REGULATE NEUROBLAST SELF-RENEWAL AND ASYMMETRIC DIVISION IN DROSOPHILA MELANOGASTER
… in neuroblasts. The other player is Drosophila cytoplasmic dynein light chain 1, also named Cut up (Ctp). Ctp localizes at centrioles and controls the mitotic spindle orientation in neuroblasts. Ctp directly interacts with a centriolar protein Anastral Spindle 2 (Ana2) and regulates the …
-
Regulation of Organelle Transport by Myosin v in the Cell Cycle
… by three motors: two microtublule motors, cytoplasmic dynein and kinesin II, and the actin motor myosin V. Here, the cell cycle regulation of myosin V was investigated. In earlier work from our lab, myosin V was found to be downregulated, as it was released from organelles during mitosis …
-
Studying Processive Molecular Motors Inside Live Cells
… motion of motor proteins conventional kinesin, cytoplasmic dynein, kinesin II and myosin V.
-
Bidirectional transport by molecular motors
… and a 'minus' end. Some molecular motors such as cytoplasmic dynein walk to the minus end, while others such as conventional kinesin walk to the plus end. Cells typically have an isopolar microtubule network. This is most pronounced in neuronal axons or fungal hyphae. In these long and thin …
-
Identification of a Novel Link between the Motor Proteins Dynein and Kinesin-1
The motor proteins dynein and kinesin are fascinating biological machines which, like vehicles in a city, move various cellular cargoes along cytoskeletal microtubules (MT). In filamentous fungi, these motors are important for the hyphal growth, which is characterized by localized extension at the …
-
Lis1 and Ndel1 Regulate Dynein-Mediated Organelle Transport In Adult Neurons
… partner, Ndel1, both bind to the motor protein cytoplasmic dynein (2). This microtubule-based motor is best known for its role in retrograde axonal transport (3), but also clearly functions with Lis1 and Ndel1 to control distinct developmental processes. If embryonic defects are solely …
-
Small Molecules as Probes for Cell Division and Intracellular Transport
… DAP-29, was identified that potentially targets cytoplasmic dynein, an essential motor protein in many transport processes. Both compounds showed activity in assays in a variety of other intracellular transport systems, highlighting their potential use as biological probes. My findings indicate …
-
Regulation of Bidirectional Microtubule-Dependent Organelle Transport in Xenopus Laevis Melanophores
… the cytoplasm by the motor proteins kinesin-2, cytoplasmic dynein, and myosin V. While the motor proteins responsible for melanosome transport have been identified, the regulatory mechanisms that control melanosome transport are less known. The following dissertation seeks to shed light on the …
-
Investigating dynein-based transport of intracellular pathogens
Cytoplasmic dynein is a major microtubule-based motor which powers the long-distance transport of organelles, vesicles and other cytoplasmic contents from the cell periphery towards the centre. While dynein typically moves cellular cargoes, it can also be hijacked by pathogens that rely on …
-
Functional analyses of mitotic microtubule-binding complexes
… optimal mitotic progression. Second, I analyze cytoplasmic dynein, a microtubule-based motor that is critically required to maintain spindle bipolarity and execute numerous other cellular processes throughout the cell cycle. The execution of these diverse functions of dynein relies on precise …
Page 1 of 2