Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 61 for “"cytoplasmic domain"”.
-
Integrin Alpha Subunit Expression and Cytoplasmic Domain Function
… and associate with the cytoskeleton via their cytoplasmic domains. By mediating the interaction of the cellular cytoskeleton with the ECM, the $\beta\sb1$ integrins are central to issues of cell migration, proliferation, differentiation, cytoskeletal organization, cell morphology, and the …
-
Role of the Type III TGF-beta Receptor Cytoplasmic Domain in Breast Cancer Progression
… effects, I examined the contribution of the cytoplasmic domain of TβRIII, which is known to be critical in the regulation of TβRIII cell surface expression and downstream signaling. <italic>In vitro</italic>, I demonstrated that abrogation of the cytoplasmic domain attenuates the …
-
The Cytoplasmic Domain of Membrane-type 1 Matrix Metalloproteinase is Required for its Survival-Promoting, but not its Migration-Promoting Function in MCF-7 Breast Cancer Cells
… capabilities of MT1-MMP depend on its cytoplasmic domain (CD), indicative of a diverse regulatory function. The effects of CD mutations on cell migration and survival were examined by ectopically expressing MT1-MMP variants in MCF-7 cells. CD alteration by substitution or deletion did …
-
A signal for retention of cytochrome P450 2C1 in the endoplasmic reticulum
… an N-glycosylation site, were fused to a soluble cytoplasmic protein, Escherichia coli $\beta$-galactosidase, or to a secreted protein, Escherichia coli alkaline phosphatase, and the hybrid proteins were expressed in COS1 cells and cellular localization was determined by subcellular fractionation, …
-
Studies of the Mechanisms of Reverse Signaling by CD40L
… of reverse signaling. A synthetic SH2-domain binding array identified Abl2, CRK, and CRKL as candidates that interact with the highly conserved phosphorylated tyrosine residue at position 5 (Y5) in the CD40L cytoplasmic domain. To assess structural requirements, CD40L constructs with …
-
Exploring the Structural Dynamics of Bacterial Chemotaxis
… which bind attractant ligands in the periplasmic domain and transmit ligand occupancy information through the cytoplasmic domain to inhibit a downstream histidine kinase that regulates motor control. Receptors are hypothesized to transmit this signal via dynamic structural changes in the >200 Å …
-
Axon/dendrite Targeting of Metabotropic Glutamate Receptors by Their Cytoplasmic Carboxy Terminal Domains
… of mGluR2 requires its 53 aa C-terminal cytoplasmic domain, whereas axon targeting of mGluR7 requires its 65 aa C-terminal cytoplasmic domain. Insertion of the mGluR7 C-terminal sequence into full length mGluR2 or into the unrelated somatodendritic protein telencephalin induced axon …
-
Characterization of structure and dynamics of membrane proteins from solid-state NMR
… membrane proteins: 1) the N-terminal ectodomain and C-terminal cytoplasmic domain of influenza A virus M2 and 2) HIV-1 glycoprotein gp4l membrane-proximal external region and transmembrane domain (MPER-TMD) in a near native membrane environment. The cytoplasmic domain of M2 is necessary …
-
Functional analysis of ICAM-1 : LFA-1 interaction in cell adhesion
… Studies were conducted to define a role for the cytoplasmic domain of ICAM-1 by transfectingICAM-1 with or without its cytoplasmic domain into ICAM-1 negative cells. These studies demonstrated that removal of the cytoplasmic domain of ICAM-1 resulted in a decreased but not abolished aggregative …
-
Identification and Function of Beta-1,4-Galactosyltransferase Associated Proteins
… proteins that interact with GalTase-I cytoplasmic domain will greatly enhance our ability to understand the signaling events activated by GalTase-I during the process of fertilization.
-
The development of peptide-based inhibitors of the low affinity Fc receptor, Fc [gamma] RIIa
… tyrosine-based activation motif (ITAM) in its cytoplasmic domain, involved in the initiation of signalling events. There is now strong evidence from transgenic mouse models and human genetic susceptibility studies that implicate FcyRIIa in a number of immune diseases including, rheumatoid …
-
Interactions between integral membrane proteins and the cytoskeleton in red blood cells studied by measured molecular lateral diffusion
… which closely associate with the bilayer on its cytoplasmic side. Consequently, receptor-protein lateral mobility is considerably lower in membranes than in lipid bilayers, presumably because of weak non-covalent interactions with peripheral membrane components. Thus the cell membrane, like many …
-
Functional analysis of murine CD43 shedding : a role for the CD43 cytoplasmic tail in nuclear signalling
… Our data support the hypothesis that CD43 ectodomain shedding is a requirement for release of the cytoplasmic domain and its translocation to the nucleus. In support of our hypothesis, we confirmed that the CD43 cytoplasmic domain is localized in the nucleus and is modified by SUMO (small …
-
A Transmembrane Precursor of Secretory Component
… microsomal vesicles with an 11-15 kilodalton domain remaining in the cytoplasm. We studied the biosynthesis and processing of human SC in a cell-free translations and pulse labelling of cells, SC is made as a single larger precursor with an approximately 15 kilodalton cytoplasmic domain. In …
-
The membrane lipid composition regulates talin mediated integrin activation
… receptors often requires the binding of the cytoplasmic domain of the subunits by talin. This interaction leads to separation of the integrin and transmembrane domain and significant conformational changes in the extracellular domains, resulting in a dramatic increase in integrin's affinity …
-
Substrate targeting and recognition in the flagellar type III secretion pathway
… proton motive force (PMF) and facilitated by a cytoplasmic ATPase complex comprising FliH, FliI and FliJ, which are evolutionarily related to components of the F1 ATPase. Unfolded subunits transit the length of the channel to the flagellum tip where they fold into the nascent structure. The …
-
Differenzielle Effekte von Presenilin-Mutationen auf die Generierung des Amyloid-ß-Peptids (Aß) und die Endoproteolyse des Notch-Rezeptors
… of Notch and nuclear transport of its cytoplasmic domain was significantly inhibited. These results demonstrate differential effects of PS proteins in the endoproteolysis of the ß-amyloid precursor protein and Notch.
-
Directed Migration Achieved in Mammalian Cells via Ca2+ Signal Rewiring in Synthetic Protein Chimeras
… upon ligand binding by creating fusions of domains that oligomerize to the transmembrane domain and the cytoplasmic tail of the VEGFR2. By coupling these chimeric proteins that generate Ca2+ signals with proteins that respond to Ca2+ signals, we re-wired, for example, dynamic cellular …
-
Dissecting the Function of FARP1 and Rho GTPases in Semaphorin-Plexin Signaling: Structural Perspective
… the dimerization of Plexin and stimulates its cytoplasmic GAP activity towards Rap, resulting in cytoskeleton re-organization. The growth cone of an axon then turns around, which ultimately leads to the repulsive guidance of the axon. Structural studies by our laboratory and others have …
-
Probing cardiac calcium regulation using time-resolved optical spectroscopy.
… of sarcoplasmic reticulum (SR), decreases the cytoplasmic Ca2+ concentration, and initiates relaxation in both skeletal and cardiac muscle. In the heart, SERCA activity is partially inhibited by phospholamban, another integral membrane protein. This inhibition can be relieved by high Ca2+ …
Page 1 of 4