Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 50 for “"cytometer"”.
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Development of the in vivo flow cytometer
An in vivo flow cytometer has been developed that allows the real-time detection and quantification of circulating cells containing fluorescent proteins or labeled with fluorochrome molecules in live animals, without the need to extract blood samples. A stationary laser beam is focused by a …
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Optimization of the mini-flo flow cytometer dc by Mukund C. Venkatesh.
Thesis (M.S.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 1996.
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Cytoflow Optics Analysis
… company. The company’s aim is to make a custom cytometer that can be used by consumers. The purpose of this project was to create a design for the optics assembly of the cytometer. Prototypes were constructed and then tested to analyze results. Data was collected then analyzed using a specific …
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Modular Neural Networks for Analysis of Flow Cytometry Data
… an automated optical tool, called a Flow Cytometer (FC), to analyse samples quickly and in large quantities. The flow cytometer has<br/>successfully been applied to real phytoplankton studies. However, analysis of the data extracted from samples is still required. Artificial Neural …
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Quantitative phase imaging : a study from 2D to 3D
… studies. By adapting QPI system into an image cytometer, termed Quantitative phase cytometer (QPC), a large population of ~ 10⁴ HeLa cells were characterized with single-cell level morphology information and dry mass histogram. Next, in order to gain more accurate information such as nuclear …
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Investigation of the genotoxic potential of the marine biotoxins okadaic acid and azaspiracids
… and assess the right concentration range. Flow cytometer analysis was included to detect the possible involvement of apoptotic processes. In house background data for all endpoints were established using positive controls. Three different cell lines, Jurkat T cells, CaCo-2 cells and HepG-2 …
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High throughput 3D optical microscopy : from image cytometry to endomicroscopy
… a high throughput depth resolved widefield image cytometer based on the structured light illumination and the high speed remote depth scanning. This system improves imaging throughput by an order of magnitude over the current technology and can potentially be applied to image cytometry …
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Micro-impedance cytometry
… is important. In this thesis, a micro-impedance cytometer for the detection of small particles and bacteria and for the discrimination of particles that are very similar in size is presented. The device consists of a microfluidic channel where two pairs of microfabricated electrodes are provided …
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Applied Plankton Image Classification for Imaging FlowCytobot Data
… (IFCB), an automated submersible flow cytometer, produces high resolution images of plankton at rates up to 10 Hz for months or years, resulting in billions of images. This project compares various methods to categorize incoming images of plankton gathered by the IFCB - Convolutional …
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Altering Nitric Oxide Bioavailability and Lipid Profiles in Endothelial Cells By Polycyclic Aromatic Hydrocarbons in Particulate Matter
… using the fluorometric method with a flow cytometer. Lipid peroxidation was assessed based on the formation of malondialdehyde. Nitric oxide synthase was calculated using a cell-permeable diacetate that reacts with NO to form a fluorescent triazolotluorescein. Then, lipids of HCAEC were …
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Intrinsic cytometry based on computational microscopy
… cells on the same platform, the intrinsic cytometer will enable a large pool of quantitative measurement data of cell intrinsic properties that can potentially be used for cell characterization and diagnostics.
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Applying a microfluidic 'deformability cytometry' to measure stiffness of malaria-infected red blood cells at body and febrile temperatures
… Experiments revealed that the deformability cytometer can differentiate parasitized RBCs from normal RBCs most efficiently at febrile temperature, suggesting a potential role of fever in facilitating splenic clearance. Similar dynamic deformability measurements were also conducted on RBCs …
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Investigating the role of CD56⁻ ‘atypical’ NK cells in Gambian children with asymptomatic malaria
… of surface and intracellular proteins. The cytometer will quantify the signals to determine the presence and intensity of marker expression. Optimization of the cytometry panel and staining protocol is critical for accurate detection, particularly for rarer cell populations such as CD56⁻ NK …
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Micro fluidic cytometry for analysis of marine microorganisms
… of the information obtained from the LOC cytometer.
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Scalable development of multiplexed microparticle technologies for optical single-cell barcoding
… modified microscope and a custom-developed flow cytometer. We expect that the proposed technology offers a platform to identify single cells in various single-cell measurements and allows the acquired data of same cells to be integrated using the optical barcodes.
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NON-CONVENTIONAL YEASTS AS CELL FACTORIES: FROM FOOD WASTE TO ADDED-VALUE PRODUCTS.
… freshwater. In this work, an analytical flow cytometer method was developed for a rapid and sustainable monitoring of lipid accumulation. - Expressing a heterologous adhesin protein (CFL1p) to enhance the flocculating ability of C. oleaginosum ATCC 20509. This reduced the time required for …
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A comparison of flow cytometry and conventional microbiology in the study of biofilms.
… river water biofilms were analysed by the flow cytometer and direct counting methods as well as by conventional microbiological methods (plate counting). The bacterial populations in real water distribution systems have been fully investigated and the total, viable bacteria were determined by …
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Spatiotemporal modeling and model restructuration approaches in studies of intracellular signalling pathways
… carried out using confocal microscopy and flow cytometer. To understand the particle uptake behavior with time and steady state condition, reaction-diffusion and kinetics model has been developed in these work. After a detailed analysis of experimental data and models, it showed that total …
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Herstellung und Charakterisierung monoklonaler Antikörper gegen equine Leukozyten
… are binding specifically to equine T-cells. Flow cytometer analyses demonstrated an increased frequency following the stimulation with Concanavalin A. The frequency of mAb 4-13- and mAb 4-58-positive cells matched the total frequency of CD4- and CD8-positive cells. The number of cells, marked by …
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A microfabricated dielectrophoretic trapping array for cell-based biological assays
… The trapping arrays have potential use as a cytometer for monitoring the dynamics of populations of single cells and then sorting those cells based upon those dynamics. To design such traps, I have developed a modeling environment that can absolutely predict the ability of DEP-based traps to …
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