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Showing 1 to 15 of 15 for “"cytolysin"”.
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Biosynthesis and mechanism of action of enterococcal cytolysin
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2023-12-01
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Mechanistic and synthetic studies on the prochlorosin and cytolysin families of lanthipeptides
… analogues. Interestingly, a lanthipeptide, cytolysin S (CylLS”), exhibited cytolytic activity in synergy with cytolysin L (CylLL”). Given that a thioether crosslink in CylLS” has an unusual LL-stereochemistry, the synthesis of a diastereomer of CylLS” with the more common DL-stereochemistry …
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Characterization Of Streptolysin O As The Translocator For The Cytolysin-Mediated Translocation Process In Streptococcus Pyogenes
Secretion of toxins into host cells is an important component of microbial pathogenesis. In order to gain access to the host cell cytosol, toxins must cross the plasma membrane, traverse the membrane of endocytic vesicles, or cross the membrane of organelles. The process …
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Mechanistic studies of lanthipeptide biosynthesis
… structural characterization of the enterococcal cytolysin, a lanthipeptide tightly linked to Enterococcus faecalis virulence. The stereoselectivity of lanthionine synthesis is discussed in chapters 3 and 4. A non-canonical configuration of lanthionines was discovered in a few lanthipeptides and …
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Engineering targeted proteins for intracellular delivery of biotherapeutics
… or cell line. Next, cholesterol-dependent cytolysins were targeted to the same antigens by genetic fusion to engineered fibronectin domains. When combined in vitro, targeted gelonin and cytolysin had synergistic cytotoxic effects and the presence of cytolysin reduced the intracellular …
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Protein engineering for cancer therapy
… agent that contains the cholesterol dependent cytolysin, perfringolysin 0, enhances endosomal escape of siRNA. Targeted delivery of perfringolysin 0 induces gene silencing in a dose-dependent and EGFR-dependent manner. However, cytotoxicity of the cytolysin creates a narrow therapeutic window. …
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Use of solution-state nuclear magnetic resonance spectroscopy to determine the structures of medicinally relevant peptides and proteins
… synthetase. Next was the two peptide lantibiotic cytolysin, unique among bacteriocins in that it has hemolytic activity in addition to bactericidal activity. Nisin, the prototypical lantibiotic, was studied in the context of the immunity protein, NisI, which is believed to confer immunity against …
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Activation State of Human T and NK Cells Increases Sensitivity to Death by Streptococcus pneumoniae
… immune system. PLY is a cholesterol-dependent cytolysin and has been shown to be a major virulence factor. In this study, we investigated the hemolytic activity of Spn lysates of several different pneumococcal serotypes. We showed the hemolytic activity of Spn correlated with CD8 T cell, CD4 T …
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CcpA-Mediated Carbon Catabolite Repression of Virulence in the Group A Streptoccus
… expression of saga, the gene encoding the potent cytolysin, streptolysin S (SLS). Moreover, hemolytic activity due to SLS was increased in the delta ccpA strain, and expression from Psaga demonstrated strong catabolite repression during growth in glucose compared to sucrose. Furthermore, purified …
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Determination of the occurrence, virulence genes, and antimicrobial resistance profiles of enterococcus faecalis and enterococcus faecium isolated from poultry of Chongwe, Lusaka, Kitwe, and Ndola districts, farmworkers from the Copperbelt province poultry farms and clinical specimens from Kitwe teaching hospital in Zambia.
… substance, enterococcal surface protein, cytolysin, pheromone cAD1 precursor lipoprotein, E. faecalis endocarditis antigen Regulator of gelE and sprE expression and collagen-binding cell wall protein in E. faecalis and E. faecium, and E. faecium specific cell wall adhesion. Overall, 95.5% …
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Organization of the Streptococcal ExPortal and its Interaction with Cationic Antimicrobial Peptides
… relevant SpeB protease and Streptolysin O: SLO) cytolysin. One of the CAPs investigated also targets the lipid II precursor of extracellular peptidoglycan, suggesting the influence of CAPs on ExPortal organization extended beyond their affinity for negatively charged membrane lipids and that the …
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Structural investigation of lanthipeptides involved in substrate controlled pathways and methods for leader peptide removal
… structures, as well as the two component cytolysin peptides, which adopt helical conformations consistent with traits previously shown to be important in membrane permeabilization by other helical peptides. These peptides were chosen because previous studies showed that the CylM-catalyzed …
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Reversibly neutralized perfringolysin O for intracellular delivery of macromolecules/
… O (PFO) is a member of the cholesterol-dependent cytolysin (CDC) family of bacterial toxins whose pores, reaching up to 30nm in diameter, allow the passage of large molecules without a specialized transport mechanism. However, the creation of such pores on the cell membrane is accompanied by …
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Untersuchungen zur Aufklärung der In-vivo-Funktion des Zytolysins ClyA von Escherichia coli
Porenbildende Zytolysine stellen wichtige Virulenzfaktoren von vielen pathogenen Bakterien dar. In Escherichia coli-Stämmen wurden bisher drei verschiedene porenbildende Zytolysine identifiziert, nämlich das alpha-Hämolysin (HlyA), das EHEC-Hämolysin (EHEC-HlyA, Ehx) und das latente Hämolysin ClyA. …
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Human Complement Protein C8: Crystal Structure and Analysis of Binding Interactions
Human C8 is part of the membrane attack complex (MAC) that assembles on bacterial cell membranes to form a lethal pore-like structure. The MAC is comprised of complement proteins C5b, C6, C7, C8 and C9. The 150-kDa C8 protein consists of three non-identical subunits (C8alpha, C8beta, C8gamma), …